Values CatLog read from this paper
Isoform-Specific NO Synthesis by Arabidopsis thaliana Nitrate Reductase
PMID 30884848doi:10.3390/plants8030067Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (20 values) from this paper: nitrate reductase NIA1, nitrate reductase NIA2 from Arabidopsis thaliana.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| nitrate reductase NIA1 | Arabidopsis thaliana | nitrateMV:nitrate assay; 25 nM NR-Mo-heme; residues 1-627 | 51 | 2.12 | 24 | pH 7 | Open record: nitrate reductase NIA1, nitrate |
| nitrate reductase NIA1 | Arabidopsis thaliana | nitrate220 µM NADH (saturating); nitrate 0-6 mM; cosubstrate NADH | 9.6 | 0.017 | 560 calc. | pH 7 | Open record: nitrate reductase NIA1, nitrate |
| nitrate reductase NIA1 | Arabidopsis thaliana | nitriteN-terminal Mo-heme fragment NIA1-Mo-heme; residues 1-627 | 19.8 | 0.0355 | 558 calc. | pH 7.5 | Open record: nitrate reductase NIA1, nitrite |
| nitrate reductase NIA1 | Arabidopsis thaliana | nitriteMoco-domain fragment NIA1-Mo; residues 1-488; Table 1 | 137.5 | 0.0353 | 3.90 × 10³ calc. | pH 7.5 | Open record: nitrate reductase NIA1, nitrite |
| nitrate reductase NIA1 | Arabidopsis thaliana | nitriteN-terminal Mo-heme fragment NIA1-Mo-heme; residues 1-627 | — | — | 557 | pH 7.5 | Open record: nitrate reductase NIA1, nitrite |
| nitrate reductase NIA2 | Arabidopsis thaliana | nitrateMV:nitrate assay; 25 nM NR-Mo-heme; residues 1-625 | 69 | 0.443 | 155 | pH 7 | Open record: nitrate reductase NIA2, nitrate |
| nitrate reductase NIA2 | Arabidopsis thaliana | nitrate220 µM NADH (saturating); nitrate 0-6 mM; cosubstrate NADH | 13.4 | 0.035 | 380 calc. | pH 7 | Open record: nitrate reductase NIA2, nitrate |
| nitrate reductase NIA2 | Arabidopsis thaliana | nitriteTable 1 | 1.8 | 0.0137 | 130 calc. | pH 7.5 | Open record: nitrate reductase NIA2, nitrite |
| nitrate reductase NIA2 | Arabidopsis thaliana | nitritein the text | — | — | 131 | pH 7.5 | Open record: nitrate reductase NIA2, nitrite |
| nitrate reductase NIA2variant H600A | Arabidopsis thaliana | nitrite | 1.2 | 0.0103 | 120 calc. | pH 7.5 | Open record: nitrate reductase NIA2 variant H600A, nitrite |