Values CatLog read from this paper
Mechanism of the Flavoprotein d-6-Hydroxynicotine Oxidase: Substrate Specificity, pH and Solvent Isotope Effects, and Roles of Key Active-Site Residues
PMID 31046245doi:10.1021/acs.biochem.9b00297Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (50 values) from this paper: D-6-hydroxynicotine oxidase from Arthrobacter nicotinovorans.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-6-hydroxynicotine oxidase | Arthrobacter nicotinovorans | (R)-4-(1-methylpyrrolidine-2-yl)phenol | 1.5 | 0.36 | 4 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase, (R)-4-(1-methylpyrrolidine-2-yl)phenol |
| D-6-hydroxynicotine oxidase | Arthrobacter nicotinovorans | (R)-6-chloronicotine | 0.85 | 0.21 | 4 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase, (R)-6-chloronicotine |
| D-6-hydroxynicotine oxidase | Arthrobacter nicotinovorans | (R)-6-hydroxynicotineTable 1 upper value row | 77 | 0.2 | 380 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase, (R)-6-hydroxynicotine |
| D-6-hydroxynicotine oxidase | Arthrobacter nicotinovorans | (R)-6-hydroxynicotine250 µM O2 (air-saturated buffer); amine concentration varied | 8.7 | 0.023 | 380 calc. | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase, (R)-6-hydroxynicotine |
| D-6-hydroxynicotine oxidase | Arthrobacter nicotinovorans | (R)-6-hydroxynornicotine | 8.1 | 0.34 | 24 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase, (R)-6-hydroxynornicotine |
| D-6-hydroxynicotine oxidase | Arthrobacter nicotinovorans | (R)-nicotine | 0.26 | 0.84 | 0.15 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase, (R)-nicotine |
| D-6-hydroxynicotine oxidase | Arthrobacter nicotinovorans | molecular oxygen | — | 4.7 | 16 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase, molecular oxygen |
| D-6-hydroxynicotine oxidasevariant E350A | Arthrobacter nicotinovorans | (R)-6-hydroxynicotineTable 1 upper value row | 20 | 1.12 | 17.8 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase variant E350A, (R)-6-hydroxynicotine |
| D-6-hydroxynicotine oxidasevariant E350A | Arthrobacter nicotinovorans | (R)-6-hydroxynicotine250 µM O2 (air-saturated buffer); amine concentration varied | 3.5 | 0.78 | 4.5 calc. | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase variant E350A, (R)-6-hydroxynicotine |
| D-6-hydroxynicotine oxidasevariant E350A | Arthrobacter nicotinovorans | molecular oxygen | — | 1.8 | 11.5 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase variant E350A, molecular oxygen |
| D-6-hydroxynicotine oxidasevariant E350Q | Arthrobacter nicotinovorans | (R)-6-hydroxynicotineTable 1 upper value row | 9.3 | 5.5 | 1.7 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase variant E350Q, (R)-6-hydroxynicotine |
| D-6-hydroxynicotine oxidasevariant E350Q | Arthrobacter nicotinovorans | (R)-6-hydroxynicotine250 µM O2 (air-saturated buffer); amine concentration varied | 5.6 | 3.3 | 1.7 calc. | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase variant E350Q, (R)-6-hydroxynicotine |
| D-6-hydroxynicotine oxidasevariant E350Q | Arthrobacter nicotinovorans | molecular oxygen | — | 0.43 | 21 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase variant E350Q, molecular oxygen |
| D-6-hydroxynicotine oxidasevariant E352Q | Arthrobacter nicotinovorans | (R)-6-hydroxynicotineTable 1 upper value row | 0.63 | 6 | 0.1 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase variant E352Q, (R)-6-hydroxynicotine |
| D-6-hydroxynicotine oxidasevariant E352Q | Arthrobacter nicotinovorans | (R)-6-hydroxynicotine250 µM O2 (air-saturated buffer); amine concentration varied | 0.76 | 7.2 | 0.11 calc. | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase variant E352Q, (R)-6-hydroxynicotine |
| D-6-hydroxynicotine oxidasevariant E352Q | Arthrobacter nicotinovorans | molecular oxygen | — | 0.2 | 3.2 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase variant E352Q, molecular oxygen |
| D-6-hydroxynicotine oxidasevariant K348M | Arthrobacter nicotinovorans | (R)-6-hydroxynicotineTable 1 upper value row | 46 | 0.32 | 144 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase variant K348M, (R)-6-hydroxynicotine |
| D-6-hydroxynicotine oxidasevariant K348M | Arthrobacter nicotinovorans | (R)-6-hydroxynicotine250 µM O2 (air-saturated buffer); amine concentration varied | 12 | 0.08 | 150 calc. | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase variant K348M, (R)-6-hydroxynicotine |
| D-6-hydroxynicotine oxidasevariant K348M | Arthrobacter nicotinovorans | (R)-6-hydroxynornicotine | 5.4 | 0.83 | 6.5 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase variant K348M, (R)-6-hydroxynornicotine |
| D-6-hydroxynicotine oxidasevariant K348M | Arthrobacter nicotinovorans | molecular oxygen | — | 1.2 | 39 | pH 8, 25 °C | Open record: D-6-hydroxynicotine oxidase variant K348M, molecular oxygen |