Values CatLog read from this paper
Four amino acids define the CO2 binding pocket of enoyl-CoA carboxylases/reductases
PMID 31243147doi:10.1073/pnas.1901471116Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (40 values) from this paper: crotonyl-CoA carboxylase/reductase (KsCcr) from Kitasatospora setae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 20 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| crotonyl-CoA carboxylase/reductase (KsCcr) | Kitasatospora setae | carbon dioxide | 78 | 0.09 | 870 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr), carbon dioxide |
| crotonyl-CoA carboxylase/reductase (KsCcr) | Kitasatospora setae | crotonyl-CoA | 103 | 0.021 | 4.9 × 10³ calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr), crotonyl-CoA |
| crotonyl-CoA carboxylase/reductase (KsCcr) | Kitasatospora setae | NADPH | 86 | 0.037 | 2.3 × 10³ calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr), NADPH |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant E171A | Kitasatospora setae | carbon dioxide | 5.1 | 0.155 | 33 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant E171A, carbon dioxide |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant E171A | Kitasatospora setae | crotonyl-CoA | 5.1 | 0.5 | 10 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant E171A, crotonyl-CoA |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant E171A | Kitasatospora setae | NADPH | 6 | 0.112 | 54 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant E171A, NADPH |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant F170A | Kitasatospora setae | crotonyl-CoA | 8.3 | 0.031 | 270 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant F170A, crotonyl-CoA |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant F170A | Kitasatospora setae | NADPH | 11 | 0.011 | 1.0 × 10³ calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant F170A, NADPH |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant F170Y | Kitasatospora setae | carbon dioxide | 56 | 0.15 | 370 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant F170Y, carbon dioxide |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant F170Y | Kitasatospora setae | crotonyl-CoA | 83 | 0.01 | 8.3 × 10³ calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant F170Y, crotonyl-CoA |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant F170Y | Kitasatospora setae | NADPH | 56 | 0.036 | 1.6 × 10³ calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant F170Y, NADPH |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant H365N | Kitasatospora setae | carbon dioxide | 7.4 | 1.31 | 5.6 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant H365N, carbon dioxide |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant H365N | Kitasatospora setae | crotonyl-CoA | 5 | 0.0298 | 170 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant H365N, crotonyl-CoA |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant H365N | Kitasatospora setae | NADPH | 8.1 | 0.022 | 370 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant H365N, NADPH |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant S119A | Kitasatospora setae | CO2 | 1.32 | 0.08 | 16 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant S119A, CO2 |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant S119A | Kitasatospora setae | crotonyl-CoA | 1.79 | 0.036 | 50 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant S119A, crotonyl-CoA |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant S119A | Kitasatospora setae | NADPH | 1.47 | 0.03 | 49 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant S119A, NADPH |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant T82D | Kitasatospora setae | CO2 | 0.17 | 0.03 | 5.7 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant T82D, CO2 |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant T82D | Kitasatospora setae | crotonyl-CoA | 0.172 | 0.0134 | 12.8 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant T82D, crotonyl-CoA |
| crotonyl-CoA carboxylase/reductase (KsCcr)variant T82D | Kitasatospora setae | NADPH | 0.157 | 0.052 | 3.0 calc. | pH 8, 30 °C | Open record: crotonyl-CoA carboxylase/reductase (KsCcr) variant T82D, NADPH |