Values CatLog read from this paper
YqfB protein from Escherichia coli: an atypical amidohydrolase active towards N4-acylcytosine derivatives
PMID 31964920doi:10.1038/s41598-020-57664-wOpen in Browse
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CatLog read 20 records (52 values) from this paper: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) | Escherichia coli | capecitabine | 0.016 | 0.7 | 0.022 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein), capecitabine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) | Escherichia coli | N4-acetyl-2'-deoxycytidine | 101 | 0.4 | 230 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein), N4-acetyl-2'-deoxycytidine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) | Escherichia coli | N4-acetyl-5-fluorocytosine | 90 | 2.1 | 40 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein), N4-acetyl-5-fluorocytosine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) | Escherichia coli | N4-acetylcytidinerecombinant tag-free YqfB (pET21b-yqfB | 196 | 0.07 | 2.8 × 10³ | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein), N4-acetylcytidine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) | Escherichia coli | N4-acetylcytidine | 154 | 0.07 | 2.5 × 10³ | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein), N4-acetylcytidine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) | Escherichia coli | N4-acetylcytidine | 157 | 0.062 | 2.2 × 10³ | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein), N4-acetylcytidine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) | Escherichia coli | N4-acetylcytosine | 70 | 0.07 | 100 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein), N4-acetylcytosine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) | Escherichia coli | N4-benzoyl-2'-deoxycytidine | 0.07 | 0.3 | 0.23 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein), N4-benzoyl-2'-deoxycytidine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) | Escherichia coli | N4-benzoylcytidine | 1.8 | 5.1 | 3.5 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein), N4-benzoylcytidine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) | Escherichia coli | N4-hexanoyl-2'-deoxycytidine | 24 | 0.14 | 72 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein), N4-hexanoyl-2'-deoxycytidine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) | Escherichia coli | N4-hexanoyl-2'-deoxycytidine 5'-triphosphate | — | — | 0.015 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein), N4-hexanoyl-2'-deoxycytidine 5'-triphosphate |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) | Escherichia coli | N4-isobutyryl-2'-deoxycytidine | 75 | 1.9 | 37.5 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein), N4-isobutyryl-2'-deoxycytidine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein)variant D27A | Escherichia coli | N4-acetylcytidine | 300 | 0.29 | 1.2 × 10³ | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) variant D27A, N4-acetylcytidine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein)variant D27A | Escherichia coli | N4-acetylcytosine | — | — | 31 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) variant D27A, N4-acetylcytosine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein)variant E74A | Escherichia coli | N4-acetylcytidine | 0.07 | 0.002 | 30 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) variant E74A, N4-acetylcytidine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein)variant E74A | Escherichia coli | N4-acetylcytosine | 0.22 | 0.3 | 0.7 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) variant E74A, N4-acetylcytosine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein)variant H70A | Escherichia coli | N4-acetylcytidine | 23 | 0.05 | 400 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) variant H70A, N4-acetylcytidine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein)variant H70A | Escherichia coli | N4-acetylcytosine | — | — | 7 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) variant H70A, N4-acetylcytosine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein)variant Y89A | Escherichia coli | N4-acetylcytidine | 40 | 2.2 | 19 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) variant Y89A, N4-acetylcytidine |
| YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein)variant Y89A | Escherichia coli | N4-acetylcytosine | — | — | 0.32 | pH 8, 22 °C | Open record: YqfB (N4-acetylcytidine amidohydrolase, ASCH domain protein) variant Y89A, N4-acetylcytosine |