Values CatLog read from this paper
Substrate Recognition and Catalytic Mechanism of the Phosphate Acyltransferase PlsX from Bacillus subtilis
PMID 32180316doi:10.1002/cbic.202000015Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 29 records (83 values) from this paper: PlsX from Bacillus subtilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| PlsX | Bacillus subtilis | holo-acyl carrier proteinTable 1 | 2.47 | 3.9 × 10⁻⁴ | 6.3 × 10³ calc. | pH 7.5, 25 °C | Open record: PlsX, holo-acyl carrier protein |
| PlsX | Bacillus subtilis | holo-acyl carrier proteinin the text | — | — | 6.3 × 10³ | pH 7.5, 25 °C | Open record: PlsX, holo-acyl carrier protein |
| PlsX | Bacillus subtilis | orthophosphate | 0.0103 | 0.104 | 0.1 | pH 7.5, 25 °C | Open record: PlsX, orthophosphate |
| PlsX | Bacillus subtilis | palmitoyl phosphate | 2.78 | 0.0037 | 750 calc. | pH 7.5, 25 °C | Open record: PlsX, palmitoyl phosphate |
| PlsX | Bacillus subtilis | palmitoyl-acyl carrier protein | 0.0127 | 0.00137 | 9.3 | pH 7.5, 25 °C | Open record: PlsX, palmitoyl-acyl carrier protein |
| PlsXvariant E181A | Bacillus subtilis | orthophosphate | 0.0043 | 0.239 | 0.03 | pH 7.5, 25 °C | Open record: PlsX variant E181A, orthophosphate |
| PlsXvariant E181A | Bacillus subtilis | palmitoyl-acyl carrier protein | 0.0045 | 0.00135 | 3.3 | pH 7.5, 25 °C | Open record: PlsX variant E181A, palmitoyl-acyl carrier protein |
| PlsXvariant E209A | Bacillus subtilis | orthophosphate | 0.0033 | 0.093 | 0.037 | pH 7.5, 25 °C | Open record: PlsX variant E209A, orthophosphate |
| PlsXvariant E209A | Bacillus subtilis | palmitoyl-acyl carrier protein | 0.0037 | 0.00124 | 3 | pH 7.5, 25 °C | Open record: PlsX variant E209A, palmitoyl-acyl carrier protein |
| PlsXvariant E236A | Bacillus subtilis | orthophosphate | 0.0045 | 0.151 | 0.03 | pH 7.5, 25 °C | Open record: PlsX variant E236A, orthophosphate |
| PlsXvariant E236A | Bacillus subtilis | palmitoyl-acyl carrier protein | 0.0042 | 0.00108 | 3.8 | pH 7.5, 25 °C | Open record: PlsX variant E236A, palmitoyl-acyl carrier protein |
| PlsXvariant K184A | Bacillus subtilis | orthophosphate | 0.0023 | 0.802 | 0.0028 | pH 7.5, 25 °C | Open record: PlsX variant K184A, orthophosphate |
| PlsXvariant K184A | Bacillus subtilis | palmitoyl-acyl carrier protein | 0.002 | 0.00149 | 1.35 | pH 7.5, 25 °C | Open record: PlsX variant K184A, palmitoyl-acyl carrier protein |
| PlsXvariant K184Q | Bacillus subtilis | orthophosphate | 0.0028 | 0.425 | 0.0067 | pH 7.5, 25 °C | Open record: PlsX variant K184Q, orthophosphate |
| PlsXvariant K184Q | Bacillus subtilis | palmitoyl-acyl carrier protein | 0.0025 | 0.00202 | 1.23 | pH 7.5, 25 °C | Open record: PlsX variant K184Q, palmitoyl-acyl carrier protein |
| PlsXvariant K184R | Bacillus subtilis | orthophosphate | 0.0068 | 0.052 | 0.132 | pH 7.5, 25 °C | Open record: PlsX variant K184R, orthophosphate |
| PlsXvariant K184R | Bacillus subtilis | palmitoyl-acyl carrier protein | 0.0067 | 0.00139 | 4.8 | pH 7.5, 25 °C | Open record: PlsX variant K184R, palmitoyl-acyl carrier protein |
| PlsXvariant K233A | Bacillus subtilis | orthophosphate | 0.0033 | 0.136 | 0.025 | pH 7.5, 25 °C | Open record: PlsX variant K233A, orthophosphate |
| PlsXvariant K233A | Bacillus subtilis | palmitoyl-acyl carrier protein | 0.003 | 0.00143 | 2.2 | pH 7.5, 25 °C | Open record: PlsX variant K233A, palmitoyl-acyl carrier protein |
| PlsXvariant N229A | Bacillus subtilis | orthophosphate | 0.001 | 0.267 | 0.0037 | pH 7.5, 25 °C | Open record: PlsX variant N229A, orthophosphate |
| PlsXvariant N229A | Bacillus subtilis | palmitoyl-acyl carrier protein | 0.001 | 0.006 | 0.17 | pH 7.5, 25 °C | Open record: PlsX variant N229A, palmitoyl-acyl carrier protein |
| PlsXvariant N229Q | Bacillus subtilis | orthophosphate | 0.0125 | 0.126 | 0.1 | pH 7.5, 25 °C | Open record: PlsX variant N229Q, orthophosphate |
| PlsXvariant N229Q | Bacillus subtilis | palmitoyl-acyl carrier protein | 0.0145 | 0.00304 | 4.8 | pH 7.5, 25 °C | Open record: PlsX variant N229Q, palmitoyl-acyl carrier protein |
| PlsXvariant R114A | Bacillus subtilis | orthophosphate | 0.0122 | 0.127 | 0.095 | pH 7.5, 25 °C | Open record: PlsX variant R114A, orthophosphate |
| PlsXvariant R114A | Bacillus subtilis | palmitoyl-acyl carrier protein | 0.012 | 0.0017 | 7 | pH 7.5, 25 °C | Open record: PlsX variant R114A, palmitoyl-acyl carrier protein |
| PlsXvariant R120A | Bacillus subtilis | orthophosphate | 0.0123 | 0.131 | 0.093 | pH 7.5, 25 °C | Open record: PlsX variant R120A, orthophosphate |
| PlsXvariant R120A | Bacillus subtilis | palmitoyl-acyl carrier protein | 0.0128 | 0.00336 | 3.8 | pH 7.5, 25 °C | Open record: PlsX variant R120A, palmitoyl-acyl carrier protein |
| PlsXvariant R73A | Bacillus subtilis | orthophosphate | 0.012 | 0.111 | 0.108 | pH 7.5, 25 °C | Open record: PlsX variant R73A, orthophosphate |
| PlsXvariant R73A | Bacillus subtilis | palmitoyl-acyl carrier protein | 0.0142 | 0.0045 | 3.2 | pH 7.5, 25 °C | Open record: PlsX variant R73A, palmitoyl-acyl carrier protein |