Values CatLog read from this paper
Identification and characterization of N9-methyltransferase involved in converting caffeine into non-stimulatory theacrine in tea
PMID 32193380doi:10.1038/s41467-020-15324-7Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (25 values) from this paper: CkTcS (theacrine synthase, N9-methyltransferase), theacrine synthase CkTcS (N9-methyltransferase), N-methyltransferase CkCS and 3 more from Camellia assamica var. kucha.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CkCS (caffeine synthase) | Camellia assamica var. kucha | 1,3,7-trimethyluric acid | 1.2 × 10⁻⁴ | 0.07149 | 0.00168 | pH 7, 27 °C | Open record: CkCS (caffeine synthase), 1,3,7-trimethyluric acid |
| CkTbS (theobromine synthase) | Camellia assamica var. kucha | 1,3,7-trimethyluric acid | 1.8 × 10⁻⁴ | 0.1622 | 0.00111 | pH 7, 27 °C | Open record: CkTbS (theobromine synthase), 1,3,7-trimethyluric acid |
| CkTcS (theacrine synthase, N9-methyltransferase) | Camellia assamica var. kucha | 1,3,7-trimethyluric acid | 0.01142 | 0.00468 | 2.440 | pH 7, 27 °C | Open record: CkTcS (theacrine synthase, N9-methyltransferase), 1,3,7-trimethyluric acid |
| CkTcS (theacrine synthase, N9-methyltransferase) | Camellia assamica var. kucha | 7-methylxanthine | 0.00607 | 0.2262 | 0.02683 | pH 7, 27 °C | Open record: CkTcS (theacrine synthase, N9-methyltransferase), 7-methylxanthine |
| CkTcS (theacrine synthase, N9-methyltransferase) | Camellia assamica var. kucha | S-adenosyl-L-methionine | 0.1861 | 0.1095 | 1.700 | pH 7, 27 °C | Open record: CkTcS (theacrine synthase, N9-methyltransferase), S-adenosyl-L-methionine |
| N-methyltransferase CkCS | Camellia assamica var. kucha | 1,3,7-trimethyluric acidFig. 2c | 1.2 × 10⁻⁴ | 0.07149 | 0.0017 calc. | pH 7, 27 °C | Open record: N-methyltransferase CkCS, 1,3,7-trimethyluric acid |
| N-methyltransferase CkCS | Camellia assamica var. kucha | 1,3,7-trimethyluric acidin the text | — | — | 0.00168 | pH 7, 27 °C | Open record: N-methyltransferase CkCS, 1,3,7-trimethyluric acid |
| N-methyltransferase CkTbS | Camellia assamica var. kucha | 1,3,7-trimethyluric acidFig. 2c | 1.8 × 10⁻⁴ | 0.1622 | 0.0011 calc. | pH 7, 27 °C | Open record: N-methyltransferase CkTbS, 1,3,7-trimethyluric acid |
| N-methyltransferase CkTbS | Camellia assamica var. kucha | 1,3,7-trimethyluric acidin the text | — | — | 0.00111 | pH 7, 27 °C | Open record: N-methyltransferase CkTbS, 1,3,7-trimethyluric acid |
| theacrine synthase CkTcS (N9-methyltransferase) | Camellia assamica var. kucha | 1,3,7-trimethyluric acidFig. 2c | 0.01142 | 0.00468 | 2.44 calc. | pH 7, 27 °C | Open record: theacrine synthase CkTcS (N9-methyltransferase), 1,3,7-trimethyluric acid |
| theacrine synthase CkTcS (N9-methyltransferase) | Camellia assamica var. kucha | 1,3,7-trimethyluric acidin the text | — | — | 2.440 | pH 7, 27 °C | Open record: theacrine synthase CkTcS (N9-methyltransferase), 1,3,7-trimethyluric acid |
| theacrine synthase CkTcS (N9-methyltransferase) | Camellia assamica var. kucha | S-adenosyl-L-methionine | — | 0.1095 | — | pH 7, 27 °C | Open record: theacrine synthase CkTcS (N9-methyltransferase), S-adenosyl-L-methionine |