Values CatLog read from this paper
Regulation of Leishmania major PAS domain-containing phosphoglycerate kinase by cofactor Mg2+ ion at neutral pH
PMID 32196942doi:10.1111/febs.15305Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 24 records (48 values) from this paper: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) from Leishmania major.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 24 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) | Leishmania major | 3-phosphoglycerate | 1.37 | 0.53 | 2.6 calc. | pH 7.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK), 3-phosphoglycerate |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) | Leishmania major | 3-phosphoglycerate | 3.7 | 0.661 | 5.6 calc. | pH 5.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK), 3-phosphoglycerate |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) | Leishmania major | ATP0.8 mM MgSO4; ATP varied at fixed 2.5 mM 3-PGA | 1.4 | 0.152 | 9.2 calc. | pH 7.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK), ATP |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) | Leishmania major | ATP0.8 mM MgSO4; ATP varied at fixed 2.5 mM 3-PGA | 3.5 | 0.118 | 30 calc. | pH 5.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK), ATP |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) | Leishmania major | ATP0.8 mM Mg2+; ATP varied (0-5 mM) at fixed 2.5 mM 3-PGA | 18 | 1 | 18 calc. | pH 5.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK), ATP |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) | Leishmania major | ATP0.8 mM Mg2+; ATP varied (0-5 mM) at fixed 2.5 mM 3-PGA | 3.2 | 0.54 | 5.9 calc. | pH 7.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK), ATP |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H111A | Leishmania major | 3-phosphoglycerate | 1.29 | 0.48 | 2.7 calc. | pH 7.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H111A, 3-phosphoglycerate |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H111A | Leishmania major | 3-phosphoglycerate | 3.8 | 0.638 | 6.0 calc. | pH 5.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H111A, 3-phosphoglycerate |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H111A | Leishmania major | ATP | 1.3 | 0.163 | 8.0 calc. | pH 7.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H111A, ATP |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H111A | Leishmania major | ATP | 3.6 | 0.128 | 28 calc. | pH 5.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H111A, ATP |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H57A | Leishmania major | 3-phosphoglycerate | 4.4 | 0.511 | 8.6 calc. | pH 7.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H57A, 3-phosphoglycerate |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H57A | Leishmania major | 3-phosphoglycerate | 3.6 | 0.61 | 5.9 calc. | pH 5.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H57A, 3-phosphoglycerate |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H57A | Leishmania major | ATP | 4.5 | 0.156 | 29 calc. | pH 7.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H57A, ATP |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H57A | Leishmania major | ATP | 3.8 | 0.134 | 28 calc. | pH 5.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H57A, ATP |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H71A | Leishmania major | 3-phosphoglycerate | 1.3 | 0.49 | 2.7 calc. | pH 7.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H71A, 3-phosphoglycerate |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H71A | Leishmania major | 3-phosphoglycerate | 3.2 | 0.68 | 4.7 calc. | pH 5.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H71A, 3-phosphoglycerate |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H71A | Leishmania major | ATP | 1.28 | 0.122 | 10.5 calc. | pH 7.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H71A, ATP |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H71A | Leishmania major | ATP | 3.3 | 0.102 | 32 calc. | pH 5.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H71A, ATP |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H89A | Leishmania major | 3-phosphoglycerate | 1.35 | 0.517 | 2.61 calc. | pH 7.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H89A, 3-phosphoglycerate |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H89A | Leishmania major | 3-phosphoglycerate | 3.2 | 0.63 | 5.1 calc. | pH 5.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H89A, 3-phosphoglycerate |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H89A | Leishmania major | ATP | 1.35 | 0.139 | 9.71 calc. | pH 7.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H89A, ATP |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant H89A | Leishmania major | ATP | 3.2 | 0.125 | 26 calc. | pH 5.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant H89A, ATP |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant Δ115 | Leishmania major | ATP | 5.8 | 0.41 | 14 calc. | pH 5.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant Δ115, ATP |
| PAS domain-containing phosphoglycerate kinase (LmPAS-PGK)variant Δ115 | Leishmania major | ATP | 14 | 2 | 7.0 calc. | pH 7.5, 25 °C | Open record: PAS domain-containing phosphoglycerate kinase (LmPAS-PGK) variant Δ115, ATP |