Values CatLog read from this paper
In vitro mutagenesis of Escherichia coli citrate synthase to clarify the locations of ligand binding sites
PMID 3276685doi:10.1016/s0021-9258(18)69186-0Open in Browse
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CatLog read 9 records (12 values) from this paper: citrate synthase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| citrate synthase | Escherichia coli | acetyl coenzyme A | — | 0.12 | — | pH 7.8, 20 °C | Open record: citrate synthase, acetyl coenzyme A |
| citrate synthase | Escherichia coli | oxaloacetateTable I | 81 | 0.026 | 3.1 × 10³ calc. | pH 7.8, 20 °C | Open record: citrate synthase, oxaloacetate |
| citrate synthase | Escherichia coli | oxaloacetatein the text | — | — | 3.1 × 10³ | pH 7.8, 20 °C | Open record: citrate synthase, oxaloacetate |
| citrate synthasevariant H226Q | Escherichia coli | acetyl coenzyme A | — | 0.26 | — | pH 7.8, 20 °C | Open record: citrate synthase variant H226Q, acetyl coenzyme A |
| citrate synthasevariant H226Q | Escherichia coli | oxaloacetateTable I | 98 | 0.017 | 5.8 × 10³ calc. | pH 7.8, 20 °C | Open record: citrate synthase variant H226Q, oxaloacetate |
| citrate synthasevariant H226Q | Escherichia coli | oxaloacetatein the text | — | — | 5.8 × 10³ | pH 7.8, 20 °C | Open record: citrate synthase variant H226Q, oxaloacetate |
| citrate synthasevariant H229Q | Escherichia coli | acetyl coenzyme A | — | 0.19 | — | pH 7.8, 20 °C | Open record: citrate synthase variant H229Q, acetyl coenzyme A |
| citrate synthasevariant H229Q | Escherichia coli | oxaloacetateTable I | 9.3 | 0.46 | 20 calc. | pH 7.8, 20 °C | Open record: citrate synthase variant H229Q, oxaloacetate |
| citrate synthasevariant H229Q | Escherichia coli | oxaloacetatein the text | — | — | 20 | pH 7.8, 20 °C | Open record: citrate synthase variant H229Q, oxaloacetate |