Values CatLog read from this paper
Enhancing the decolorization activity of Bacillus pumilus W3 CotA-laccase to Reactive Black 5 by site-saturation mutagenesis
PMID 32918582doi:10.1007/s00253-020-10897-1Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (36 values) from this paper: CotA-laccase from Bacillus pumilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CotA-laccase | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 40.11 | 0.253 | 158.5 | pH 3.6, 50 °C | Open record: CotA-laccase, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccase | Bacillus pumilus | Reactive Black 5 | 3.63 | 0.519 | 6.99 | pH 10, 37 °C | Open record: CotA-laccase, Reactive Black 5 |
| CotA-laccasevariant Q442A | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 54.38 | 0.14 | 388.4 | pH 3.6, 50 °C | Open record: CotA-laccase variant Q442A, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant Q442A | Bacillus pumilus | Reactive Black 5 | 5.73 | 0.323 | 17.74 | pH 10, 37 °C | Open record: CotA-laccase variant Q442A, Reactive Black 5 |
| CotA-laccasevariant T415D | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 50.66 | 0.227 | 223.2 | pH 3.6, 50 °C | Open record: CotA-laccase variant T415D, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant T415D | Bacillus pumilus | Reactive Black 5 | 4.46 | 0.443 | 10.07 | pH 10, 37 °C | Open record: CotA-laccase variant T415D, Reactive Black 5 |
| CotA-laccasevariant T415D/Q442A | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 36.03 | 0.101 | 356.7 | pH 3.6, 50 °C | Open record: CotA-laccase variant T415D/Q442A, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant T415D/Q442A | Bacillus pumilus | Reactive Black 5 | 5.56 | 0.298 | 18.67 | pH 10, 37 °C | Open record: CotA-laccase variant T415D/Q442A, Reactive Black 5 |
| CotA-laccasevariant T418K | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 85 | 0.261 | 326 | pH 3.6, 50 °C | Open record: CotA-laccase variant T418K, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant T418K | Bacillus pumilus | Reactive Black 5 | 4.81 | 0.428 | 11.23 | pH 10, 37 °C | Open record: CotA-laccase variant T418K, Reactive Black 5 |
| CotA-laccasevariant T418K/Q442A | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 92.16 | 0.109 | 845.5 | pH 3.6, 50 °C | Open record: CotA-laccase variant T418K/Q442A, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant T418K/Q442A | Bacillus pumilus | Reactive Black 5 | 4.78 | 0.212 | 22.54 | pH 10, 37 °C | Open record: CotA-laccase variant T418K/Q442A, Reactive Black 5 |