CatLogenzyme kinetics catalog

Values CatLog read from this paper

Engineering the Turnover Stability of Cellobiose Dehydrogenase toward Long-Term Bioelectronic Applications

PMID 34306835doi:10.1021/acssuschemeng.1c01165Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 56 records (104 values) from this paper: cellobiose dehydrogenase (ChCDH) from Crassicarpon hotsonii.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 48 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.99.18

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
cellobiose dehydrogenase (ChCDH)Crassicarpon hotsoniiglucoseDCIP (2,6-dichloroindophenol)17.41380.126 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)Crassicarpon hotsoniiglucosecyt c (cytochrome c from equine heart)6.3730.086 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)Crassicarpon hotsoniiglucoseair-saturated O_2 as electron acceptor; O_2 (air saturation)0.105810.0013 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R (referred to as WT)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme17.41380.126 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R (referred to as WT)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme6.3730.086 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R (referred to as WT)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme0.105810.0013 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R (referred to as WT)Crassicarpon hotsoniiglucose37 °C; recombinant from Komagataella phaffii—36—pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/L324C (L324C)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme8.62050.042 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/L324C (L324C)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme3.11300.024 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/L324C (L324C)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme0.0491184.2 × 10⁻⁴ calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/L324C (L324C)Crassicarpon hotsoniiglucose37 °C; recombinant from Komagataella phaffii—86—pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/L324C/M409S (L324C/M409S)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme3.72190.017 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/L324C/M409S (L324C/M409S)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme1.21200.010 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/L324C/M409S (L324C/M409S)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme0.012110.0011 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/L324C/M409S (L324C/M409S)Crassicarpon hotsoniiglucose37 °C; recombinant from Komagataella phaffii—129—pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309K (M309K)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme132000.065 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309K (M309K)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme6.21340.046 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309K (M309K)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme0.073720.0010 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309K (M309K)Crassicarpon hotsoniiglucose37 °C; recombinant from Komagataella phaffii—42—pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309K/L324C (M309K/L324C)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme3.13500.0089 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309K/L324C (M309K/L324C)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme1.22450.0049 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309K/L324C (M309K/L324C)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme0.012235.2 × 10⁻⁴ calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309K/L324C (M309K/L324C)Crassicarpon hotsoniiglucose37 °C; recombinant from Komagataella phaffii—112—pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309K/M409S (M309K/M409S)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme1.6440.036 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309K/M409S (M309K/M409S)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme0.5310.016 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309K/M409S (M309K/M409S)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme0.01390.0014 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309K/M409S (M309K/M409S)Crassicarpon hotsoniiglucose37 °C; recombinant from Komagataella phaffii—28—pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309V (M309V)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme15.11960.0770 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309V (M309V)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme4.2560.075 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309V (M309V)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme0.1221309.4 × 10⁻⁴ calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M309V (M309V)Crassicarpon hotsoniiglucose37 °C; recombinant from Komagataella phaffii—44—pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M409S (M409S)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme7.1840.085 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M409S (M409S)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme3480.062 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M409S (M409S)Crassicarpon hotsoniiglucose11 mM PBS buffer (potassium phosphate); purified enzyme0.039419.5 × 10⁻⁴ calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant C291Y/W295R/M409S (M409S)Crassicarpon hotsoniiglucose37 °C; recombinant from Komagataella phaffii—41—pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant L324CCrassicarpon hotsoniiglucoseDCIP (2,6-dichloroindophenol)8.62050.042 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant L324CCrassicarpon hotsoniiglucosecyt c (cytochrome c from equine heart)3.11300.024 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant L324CCrassicarpon hotsoniiglucoseair-saturated O_2 as electron acceptor; O_2 (air saturation)0.0491184.2 × 10⁻⁴ calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant L324C/M409SCrassicarpon hotsoniiglucoseDCIP (2,6-dichloroindophenol)3.72190.017 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant L324C/M409SCrassicarpon hotsoniiglucosecyt c (cytochrome c from equine heart)1.21200.010 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant L324C/M409SCrassicarpon hotsoniiglucoseair-saturated O_2 as electron acceptor; O_2 (air saturation)0.012110.0011 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M309KCrassicarpon hotsoniiglucoseDCIP (2,6-dichloroindophenol)132000.065 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M309KCrassicarpon hotsoniiglucosecyt c (cytochrome c from equine heart)6.21340.046 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M309KCrassicarpon hotsoniiglucoseair-saturated O_2 as electron acceptor; O_2 (air saturation)0.073720.0010 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M309K/L324CCrassicarpon hotsoniiglucoseDCIP (2,6-dichloroindophenol)3.13500.0089 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M309K/L324CCrassicarpon hotsoniiglucosecyt c (cytochrome c from equine heart)1.22450.0049 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M309K/L324CCrassicarpon hotsoniiglucoseair-saturated O_2 as electron acceptor; O_2 (air saturation)0.012235.2 × 10⁻⁴ calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M309K/M409SCrassicarpon hotsoniiglucoseDCIP (2,6-dichloroindophenol)1.6440.036 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M309K/M409SCrassicarpon hotsoniiglucosecyt c (cytochrome c from equine heart)0.5310.016 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M309K/M409SCrassicarpon hotsoniiglucoseair-saturated O_2 as electron acceptor; O_2 (air saturation)0.01390.0014 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M309VCrassicarpon hotsoniiglucoseDCIP (2,6-dichloroindophenol)15.11960.0770 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M309VCrassicarpon hotsoniiglucosecyt c (cytochrome c from equine heart)4.2560.075 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M309VCrassicarpon hotsoniiglucoseair-saturated O_2 as electron acceptor; O_2 (air saturation)0.1221309.4 × 10⁻⁴ calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M409SCrassicarpon hotsoniiglucoseDCIP (2,6-dichloroindophenol)7.1840.085 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M409SCrassicarpon hotsoniiglucosecyt c (cytochrome c from equine heart)3480.062 calc.pH 7.4, 37 °COpen
cellobiose dehydrogenase (ChCDH)variant M409SCrassicarpon hotsoniiglucoseair-saturated O_2 as electron acceptor; O_2 (air saturation)0.039419.5 × 10⁻⁴ calc.pH 7.4, 37 °COpen