Values CatLog read from this paper
Impact of pseudouridylation, substrate fold, and degradosome organization on the endonuclease activity of RNase E
PMID 34341070doi:10.1261/rna.078840.121Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (36 values) from this paper: RNase E from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| RNase E | Escherichia coli | 5'-monophosphorylated 9S RNA (precursor of 5S rRNA)enzyme 125 nM NTD; catalytic domain (NTD) | 0.01155 | 8.58 × 10⁻⁵ | 134 | pH 7.5, 30 °C | Open record: RNase E, 5'-monophosphorylated 9S RNA (precursor of 5S rRNA) |
| RNase E | Escherichia coli | 5'-monophosphorylated 9S RNA (precursor of 5S rRNA)enzyme 50 nM truncated degradosome | 0.04537 | 1.163 × 10⁻⁴ | 389 | pH 7.5, 30 °C | Open record: RNase E, 5'-monophosphorylated 9S RNA (precursor of 5S rRNA) |
| RNase E | Escherichia coli | 5'-monophosphorylated 9S RNA (precursor of 5S rRNA)enzyme 25 nM full degradosome; enolase and PNPase | 0.09316 | 1.211 × 10⁻⁴ | 837 | pH 7.5, 30 °C | Open record: RNase E, 5'-monophosphorylated 9S RNA (precursor of 5S rRNA) |
| RNase E | Escherichia coli | 5'-triphosphorylated 9S RNA (precursor of 5S rRNA)enzyme 125 nM NTD; catalytic domain (NTD) | 0.00723 | 7.54 × 10⁻⁵ | 95 | pH 7.5, 30 °C | Open record: RNase E, 5'-triphosphorylated 9S RNA (precursor of 5S rRNA) |
| RNase E | Escherichia coli | 5'-triphosphorylated 9S RNA (precursor of 5S rRNA)enzyme 50 nM truncated degradosome | 0.02105 | 6.11 × 10⁻⁵ | 343 | pH 7.5, 30 °C | Open record: RNase E, 5'-triphosphorylated 9S RNA (precursor of 5S rRNA) |
| RNase E | Escherichia coli | 5'-triphosphorylated 9S RNA (precursor of 5S rRNA)enzyme 25 nM full degradosome; enolase and PNPase | 0.08399 | 9.53 × 10⁻⁵ | 880 | pH 7.5, 30 °C | Open record: RNase E, 5'-triphosphorylated 9S RNA (precursor of 5S rRNA) |
| RNase E | Escherichia coli | 5'-triphosphorylated GlmZ small regulatory RNAenzyme 125 nM NTD; catalytic domain (NTD) | 0.03849 | 5.214 × 10⁻⁴ | 72 | pH 7.5, 30 °C | Open record: RNase E, 5'-triphosphorylated GlmZ small regulatory RNA |
| RNase E | Escherichia coli | 5'-triphosphorylated GlmZ small regulatory RNAenzyme 50 nM truncated degradosome | 0.1404 | 5.453 × 10⁻⁴ | 256 | pH 7.5, 30 °C | Open record: RNase E, 5'-triphosphorylated GlmZ small regulatory RNA |
| RNase E | Escherichia coli | 5'-triphosphorylated GlmZ small regulatory RNAenzyme 25 nM full degradosome; enolase and PNPase | 0.2863 | 6.792 × 10⁻⁴ | 421 | pH 7.5, 30 °C | Open record: RNase E, 5'-triphosphorylated GlmZ small regulatory RNA |
| RNase Evariant D26N/D28N/D338N | Escherichia coli | 5'-monophosphorylated 9S RNA (precursor of 5S rRNA) | 0.02296 | 9.69 × 10⁻⁵ | 235 | pH 7.5, 30 °C | Open record: RNase E variant D26N/D28N/D338N, 5'-monophosphorylated 9S RNA (precursor of 5S rRNA) |
| RNase Evariant D26N/D28N/D338N | Escherichia coli | 5'-triphosphorylated 9S RNA (precursor of 5S rRNA) | 0.01109 | 4.18 × 10⁻⁵ | 265 | pH 7.5, 30 °C | Open record: RNase E variant D26N/D28N/D338N, 5'-triphosphorylated 9S RNA (precursor of 5S rRNA) |
| RNase Evariant D26N/D28N/D338N | Escherichia coli | 5'-triphosphorylated GlmZ small regulatory RNA | 0.09345 | 7.29 × 10⁻⁴ | 127 | pH 7.5, 30 °C | Open record: RNase E variant D26N/D28N/D338N, 5'-triphosphorylated GlmZ small regulatory RNA |