CatLogenzyme kinetics catalog

Values CatLog read from this paper

Discovery of Two Novel Oxidases Using a High-Throughput Activity Screen

PMID 34709726doi:10.1002/cbic.202100510Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 22 records (42 values) from this paper: N-acetyl-D-hexosamine oxidase (HexNAcO), long-chain alcohol oxidase (LCAO) from Ralstonia solanacearum, uncultured marine group II/III euryarchaeota KM3_72_H01, uncultured marine group II/III euryarchaeote KM3_72_H01.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.3.20, EC 1.1.3.29

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
long-chain alcohol oxidase (LCAO)uncultured marine group II/III euryarchaeota KM3_72_H011-dodecanol—0.0277—pH 8.5, 25 °COpen
long-chain alcohol oxidase (LCAO)uncultured marine group II/III euryarchaeote KM3_72_H011-dodecanol—0.0277—pH 8.5, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumchitobiose—19—pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumD-galactosaminecoupled assay: Amplex Red/HRP H2O2 detection—1.4—pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumD-galactosamine25 °C; C-terminal linker-His tag (AAALEHHHH)0.621.350.4593pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumD-galactosecoupled assay: Amplex Red/HRP H2O2 detection—102—pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumD-galactose25 °C; C-terminal linker-His tag (AAALEHHHH)0.19101.70.0019pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumD-glucosaminecoupled assay: Amplex Red/HRP H2O2 detection—4.5—pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumD-glucosamine25 °C; C-terminal linker-His tag (AAALEHHHH)0.354.540.0771pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumD-glucosecoupled assay: Amplex Red/HRP H2O2 detection—216—pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumD-glucose25 °C; C-terminal linker-His tag (AAALEHHHH)0.16216.67 × 10⁻⁴pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumD-mannosaminecoupled assay: Amplex Red/HRP H2O2 detection—65—pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumD-mannosamine25 °C; C-terminal linker-His tag (AAALEHHHH)0.01165.72 × 10⁻⁴pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumD-mannosecoupled assay: Amplex Red/HRP H2O2 detection—118—pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumD-mannose25 °C; C-terminal linker-His tag (AAALEHHHH)0.14118.50.0012pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumN,N'-diacetylchitobiose0.8418.70.0449pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumN-acetyl-D-galactosaminecoupled assay: Amplex Red/HRP H2O2 detection—0.32—pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumN-acetyl-D-galactosamine25 °C; C-terminal linker-His tag (AAALEHHHH)4.460.3213.94pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumN-acetyl-D-glucosaminecoupled assay: Amplex Red/HRP H2O2 detection—0.26—pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumN-acetyl-D-glucosamine25 °C; C-terminal linker-His tag (AAALEHHHH)5.670.2621.81pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumN-acetyl-D-mannosaminecoupled assay: Amplex Red/HRP H2O2 detection—182—pH 7.4, 25 °COpen
N-acetyl-D-hexosamine oxidase (HexNAcO)Ralstonia solanacearumN-acetyl-D-mannosamine25 °C; C-terminal linker-His tag (AAALEHHHH)2.23181.80.0123pH 7.4, 25 °COpen