Values CatLog read from this paper
Escherichia coli alanyl-tRNA synthetase maintains proofreading activity and translational accuracy under oxidative stress
PMID 35065077doi:10.1016/j.jbc.2022.101601Open in Browse
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CatLog read 12 records (36 values) from this paper: alanyl-tRNA synthetase (AlaRS) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alanyl-tRNA synthetase (AlaRS) | Escherichia coli | alaninenonoxidized enzyme | 16.03 | 0.329 | 48 | pH 7.2, 37 °C | Open record: alanyl-tRNA synthetase (AlaRS), alanine |
| alanyl-tRNA synthetase (AlaRS) | Escherichia coli | alanineenzyme oxidized by pretreatment with 5 mM H2O2 at 37 °C | 26.7 | 0.355 | 75 | pH 7.2, 37 °C | Open record: alanyl-tRNA synthetase (AlaRS), alanine |
| alanyl-tRNA synthetase (AlaRS) | Escherichia coli | tRNA^{Ala}nonoxidized enzyme | 0.165 | 0.0011 | 150 | pH 7.2, 37 °C | Open record: alanyl-tRNA synthetase (AlaRS), tRNA^{Ala} |
| alanyl-tRNA synthetase (AlaRS) | Escherichia coli | tRNA^{Ala}enzyme oxidized by pretreatment with 5 mM H2O2 at 37 °C | 0.062 | 7 × 10⁻⁴ | 92 | pH 7.2, 37 °C | Open record: alanyl-tRNA synthetase (AlaRS), tRNA^{Ala} |
| alanyl-tRNA synthetase (AlaRS)variant C666A | Escherichia coli | tRNA^{Ala}nonoxidized enzyme | 0.038 | 0.0016 | 25 | pH 7.2, 37 °C | Open record: alanyl-tRNA synthetase (AlaRS) variant C666A, tRNA^{Ala} |
| alanyl-tRNA synthetase (AlaRS)variant C666A | Escherichia coli | tRNA^{Ala}enzyme oxidized by pretreatment with 5 mM H2O2 at 37 °C | 0.038 | 0.002 | 18 | pH 7.2, 37 °C | Open record: alanyl-tRNA synthetase (AlaRS) variant C666A, tRNA^{Ala} |
| alanyl-tRNA synthetase (AlaRS)variant M217A | Escherichia coli | tRNA^{Ala}nonoxidized enzyme | 0.115 | 9 × 10⁻⁴ | 130 | pH 7.2, 37 °C | Open record: alanyl-tRNA synthetase (AlaRS) variant M217A, tRNA^{Ala} |
| alanyl-tRNA synthetase (AlaRS)variant M217A | Escherichia coli | tRNA^{Ala}enzyme oxidized by pretreatment with 5 mM H2O2 at 37 °C | 0.112 | 0.0013 | 85 | pH 7.2, 37 °C | Open record: alanyl-tRNA synthetase (AlaRS) variant M217A, tRNA^{Ala} |
| alanyl-tRNA synthetase (AlaRS)variant M658A | Escherichia coli | tRNA^{Ala}nonoxidized enzyme | 0.192 | 0.0017 | 110 | pH 7.2, 37 °C | Open record: alanyl-tRNA synthetase (AlaRS) variant M658A, tRNA^{Ala} |
| alanyl-tRNA synthetase (AlaRS)variant M658A | Escherichia coli | tRNA^{Ala}enzyme oxidized by pretreatment with 5 mM H2O2 at 37 °C | 0.073 | 7 × 10⁻⁴ | 100 | pH 7.2, 37 °C | Open record: alanyl-tRNA synthetase (AlaRS) variant M658A, tRNA^{Ala} |
| alanyl-tRNA synthetase (AlaRS)variant M785A | Escherichia coli | tRNA^{Ala}nonoxidized enzyme | 0.092 | 0.0012 | 80 | pH 7.2, 37 °C | Open record: alanyl-tRNA synthetase (AlaRS) variant M785A, tRNA^{Ala} |
| alanyl-tRNA synthetase (AlaRS)variant M785A | Escherichia coli | tRNA^{Ala}enzyme oxidized by pretreatment with 5 mM H2O2 at 37 °C | 0.09 | 0.0019 | 48 | pH 7.2, 37 °C | Open record: alanyl-tRNA synthetase (AlaRS) variant M785A, tRNA^{Ala} |