CatLogenzyme kinetics catalog

Values CatLog read from this paper

Chromohalobacter salixigens Uronate Dehydrogenase: Directed Evolution for Improved Thermal Stability and Mutant CsUDH-inc X-ray Crystal Structure

PMID 35462854doi:10.1016/j.procbio.2020.02.013Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 34 records (102 values) from this paper: uronate dehydrogenase (CsUDH) from Chromohalobacter salixigens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.203

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
uronate dehydrogenase (CsUDH)Chromohalobacter salixigensD-galacturonic acid30.80.18172pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)Chromohalobacter salixigensD-glucuronic acid2631.6161pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant A172VChromohalobacter salixigensD-galacturonic acid270.18151pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant A172VChromohalobacter salixigensD-glucuronic acid2251.7136pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant CsUDH-inc (Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175L/D246N)Chromohalobacter salixigensD-galacturonic acid170.2178pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant CsUDH-inc (Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175L/D246N)Chromohalobacter salixigensD-glucuronic acid500.6577pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant CsUDH158 (L158A/N159C/I160V)Chromohalobacter salixigensD-galacturonic acid270.14187pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant CsUDH158 (L158A/N159C/I160V)Chromohalobacter salixigensD-glucuronic acid2191.2186pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant CsUDH99 (N99K/L100H/K102V/P103K/I105V)Chromohalobacter salixigensD-galacturonic acid170.13125pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant CsUDH99 (N99K/L100H/K102V/P103K/I105V)Chromohalobacter salixigensD-glucuronic acid1420.9162pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant CsUDH99.Y.TAMD (N99K/L100H/K102V/P103K/I105V/T113V/Y116F/A172V/M175L/D246N)Chromohalobacter salixigensD-galacturonic acid200.16126pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant CsUDH99.Y.TAMD (N99K/L100H/K102V/P103K/I105V/T113V/Y116F/A172V/M175L/D246N)Chromohalobacter salixigensD-glucuronic acid590.43136pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant D246NChromohalobacter salixigensD-galacturonic acid220.18126pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant D246NChromohalobacter salixigensD-glucuronic acid1921.3146pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant I114TChromohalobacter salixigensD-galacturonic acid170.4141pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant I114TChromohalobacter salixigensD-glucuronic acid1553.248pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant I88LChromohalobacter salixigensD-galacturonic acid220.2112pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant I88LChromohalobacter salixigensD-glucuronic acid1921.5125pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant M175LChromohalobacter salixigensD-galacturonic acid200.18116pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant M175LChromohalobacter salixigensD-glucuronic acid1531.03149pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant Q55RChromohalobacter salixigensD-galacturonic acid280.19153pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant Q55RChromohalobacter salixigensD-glucuronic acid2701.7161pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant T113VChromohalobacter salixigensD-galacturonic acid200.15132pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant T113VChromohalobacter salixigensD-glucuronic acid1921.7115pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant TAD (T113V/A172V/D246N)Chromohalobacter salixigensD-galacturonic acid300.22138pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant TAD (T113V/A172V/D246N)Chromohalobacter salixigensD-glucuronic acid2141.5139pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant TAM (T113V/A172V/M175L)Chromohalobacter salixigensD-galacturonic acid190.18104pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant TAM (T113V/A172V/M175L)Chromohalobacter salixigensD-glucuronic acid1191.1111pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant TAMD (T113V/A172V/M175L/D246N)Chromohalobacter salixigensD-galacturonic acid220.18125pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant TAMD (T113V/A172V/M175L/D246N)Chromohalobacter salixigensD-glucuronic acid1300.95138pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant TMD (T113V/M175L/D246N)Chromohalobacter salixigensD-galacturonic acid290.19155pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant TMD (T113V/M175L/D246N)Chromohalobacter salixigensD-glucuronic acid1791.1164pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant Y116FChromohalobacter salixigensD-galacturonic acid250.22111pH 8, 25 °COpen
uronate dehydrogenase (CsUDH)variant Y116FChromohalobacter salixigensD-glucuronic acid2002101pH 8, 25 °COpen