CatLogenzyme kinetics catalog

Values CatLog read from this paper

Kinetic characterization of yeast alcohol dehydrogenases. Amino acid residue 294 and substrate specificity

PMID 3546317doi:10.1016/s0021-9258(18)61419-xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 40 records (67 values) from this paper: alcohol dehydrogenase I, alcohol dehydrogenase III, alcohol dehydrogenase II and 1 more from Saccharomyces cerevisiae, Schizosaccharomyces pombe.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 27 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.1, EC 1.1.1.54, EC 1.1.1.78

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
alcohol dehydrogenaseSchizosaccharomyces pombeacetaldehyde2.7 × 10³1.61.7 × 10³ calc.pH 7.3, 30 °COpen
alcohol dehydrogenaseSchizosaccharomyces pombeethanol6201444 calc.pH 7.3, 30 °COpen
alcohol dehydrogenaseSchizosaccharomyces pombeNAD+—0.16—pH 7.3, 30 °COpen
alcohol dehydrogenaseSchizosaccharomyces pombeNADH—0.1—pH 7.3, 30 °COpen
alcohol dehydrogenase ISaccharomyces cerevisiae1-butanol51550.93 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase ISaccharomyces cerevisiae1-hexanol169.51.7 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase ISaccharomyces cerevisiae1-pentanol29370.78 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase ISaccharomyces cerevisiae1-propanol120274.4 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase ISaccharomyces cerevisiaeacetaldehyde1.7 × 10³1.11.5 × 10³ calc.pH 7.3, 30 °COpen
alcohol dehydrogenase ISaccharomyces cerevisiaeethanol3401720 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase ISaccharomyces cerevisiaeNAD+—0.17—pH 7.3, 30 °COpen
alcohol dehydrogenase ISaccharomyces cerevisiaeNADH—0.11—pH 7.3, 30 °COpen
alcohol dehydrogenase Ivariant M270LSaccharomyces cerevisiae1-butanol79117.2 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase Ivariant M270LSaccharomyces cerevisiae1-hexanol573.317 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase Ivariant M270LSaccharomyces cerevisiae1-pentanol728.88.2 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase Ivariant M270LSaccharomyces cerevisiae1-propanol120206.0 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase Ivariant M270LSaccharomyces cerevisiaeacetaldehyde2.1 × 10³12.1 × 10³ calc.pH 7.3, 30 °COpen
alcohol dehydrogenase Ivariant M270LSaccharomyces cerevisiaebutanal1.4 × 10³2.1670 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase Ivariant M270LSaccharomyces cerevisiaeethanol5001926 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase Ivariant M270LSaccharomyces cerevisiaeNAD+cosubstrate ethanol—0.63—pH 7.3, 30 °COpen
alcohol dehydrogenase Ivariant M270LSaccharomyces cerevisiaeNAD+cosubstrate butanol—0.81—pH 7.3, 30 °COpen
alcohol dehydrogenase Ivariant M270LSaccharomyces cerevisiaeNADHcosubstrate acetaldehyde—0.08—pH 7.3, 30 °COpen
alcohol dehydrogenase Ivariant M270LSaccharomyces cerevisiaeNADHcosubstrate butyraldehyde—0.07—pH 7.3, 30 °COpen
alcohol dehydrogenase IISaccharomyces cerevisiae1-butanol982.934 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase IISaccharomyces cerevisiae1-hexanol711.451 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase IISaccharomyces cerevisiae1-pentanol883.823 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase IISaccharomyces cerevisiae1-propanol892.634 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase IISaccharomyces cerevisiaeacetaldehyde1.04 × 10³0.091.2 × 10⁴ calc.pH 7.3, 30 °COpen
alcohol dehydrogenase IISaccharomyces cerevisiaeethanol1300.81160 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase IISaccharomyces cerevisiaeNAD+—0.11—pH 7.3, 30 °COpen
alcohol dehydrogenase IISaccharomyces cerevisiaeNADH—0.05—pH 7.3, 30 °COpen
alcohol dehydrogenase IIISaccharomyces cerevisiae1-butanol927.712 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase IIISaccharomyces cerevisiae1-hexanol311.916 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase IIISaccharomyces cerevisiae1-pentanol475.29.0 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase IIISaccharomyces cerevisiae1-propanol88118.0 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase IIISaccharomyces cerevisiaeacetaldehyde2.1 × 10³0.444.8 × 10³ calc.pH 7.3, 30 °COpen
alcohol dehydrogenase IIISaccharomyces cerevisiaeethanolsteady-state initial-velocity and product inhibition studies4501238 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase IIISaccharomyces cerevisiaeethanolalcohol varied over a 5-fold range; NAD+ fixed at 2 mM490——pH 7.3, 30 °COpen
alcohol dehydrogenase IIISaccharomyces cerevisiaeNAD+—0.24—pH 7.3, 30 °COpen
alcohol dehydrogenase IIISaccharomyces cerevisiaeNADH—0.07—pH 7.3, 30 °COpen