Values CatLog read from this paper

Structural insights into catalytical capability for CPT11 hydrolysis and substrate specificity of a novel marine microbial carboxylesterase, E93

PMID 36756200doi:10.3389/fmicb.2022.1081094Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 29 records (65 values) from this paper: carboxylesterase E93 from Altererythrobacter indicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.1.1.1

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
carboxylesterase E93	Altererythrobacter indicus	7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin	6.68 × 10⁻⁴	0.07017	0.0095	pH 7.4, 37 °C	Open record: carboxylesterase E93, 7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin
carboxylesterase E93	Altererythrobacter indicus	7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin	5.9 × 10⁻⁴	0.07566	0.0078	pH 7.4, 37 °C	Open record: carboxylesterase E93, 7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin
carboxylesterase E93	Altererythrobacter indicus	p-nitrophenyl butyrate	—	0.21	—	pH 7.5, 45 °C	Open record: carboxylesterase E93, p-nitrophenyl butyrate
carboxylesterase E93	Altererythrobacter indicus	p-nitrophenyl hexanoate	—	0.18	—	pH 7.5	Open record: carboxylesterase E93, p-nitrophenyl hexanoate
carboxylesterase E93variant E428A	Altererythrobacter indicus	7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin	6.668 × 10⁻⁴	0.06962	0.0095	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant E428A, 7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin
carboxylesterase E93variant E428A	Altererythrobacter indicus	7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin	3.967 × 10⁻⁴	0.08507	0.0047	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant E428A, 7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin
carboxylesterase E93variant E428A	Altererythrobacter indicus	p-nitrophenyl hexanoate	—	0.18	—	pH 7.5	Open record: carboxylesterase E93 variant E428A, p-nitrophenyl hexanoate
carboxylesterase E93variant M273A	Altererythrobacter indicus	7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin	0.001288	0.05805	0.0222	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant M273A, 7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin
carboxylesterase E93variant M273A	Altererythrobacter indicus	7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin	0.001182	0.06611	0.0178	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant M273A, 7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin
carboxylesterase E93variant M273A	Altererythrobacter indicus	p-nitrophenyl hexanoate	—	0.39	—	pH 7.5	Open record: carboxylesterase E93 variant M273A, p-nitrophenyl hexanoate
carboxylesterase E93variant Q193A	Altererythrobacter indicus	7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin	9.02 × 10⁻⁵	0.08101	0.00112	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant Q193A, 7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin
carboxylesterase E93variant Q193A	Altererythrobacter indicus	7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin	5.115 × 10⁻⁴	0.07129	0.0072	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant Q193A, 7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin
carboxylesterase E93variant Q193A	Altererythrobacter indicus	p-nitrophenyl hexanoate	—	0.1	—	pH 7.5	Open record: carboxylesterase E93 variant Q193A, p-nitrophenyl hexanoate
carboxylesterase E93variant T375A	Altererythrobacter indicus	7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin	3.183 × 10⁻⁴	0.0742	0.0043	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant T375A, 7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin
carboxylesterase E93variant T375A	Altererythrobacter indicus	7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin	5.36 × 10⁻⁴	0.07509	0.0072	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant T375A, 7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin
carboxylesterase E93variant T375A	Altererythrobacter indicus	p-nitrophenyl hexanoate	—	0.13	—	pH 7.5	Open record: carboxylesterase E93 variant T375A, p-nitrophenyl hexanoate
carboxylesterase E93variant T375I	Altererythrobacter indicus	7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin	3.575 × 10⁻⁴	0.07539	0.0047	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant T375I, 7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin
carboxylesterase E93variant T375I	Altererythrobacter indicus	7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin	5.178 × 10⁻⁴	0.07514	0.0068	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant T375I, 7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin
carboxylesterase E93variant T375I	Altererythrobacter indicus	p-nitrophenyl butyrate	—	0.1	—	pH 7.5, 45 °C	Open record: carboxylesterase E93 variant T375I, p-nitrophenyl butyrate
carboxylesterase E93variant T375I	Altererythrobacter indicus	p-nitrophenyl hexanoate	—	0.11	—	pH 7.5	Open record: carboxylesterase E93 variant T375I, p-nitrophenyl hexanoate
carboxylesterase E93variant W107A	Altererythrobacter indicus	7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin	0.001742	0.05963	0.0292	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant W107A, 7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin
carboxylesterase E93variant W107A	Altererythrobacter indicus	7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin	7.468 × 10⁻⁴	0.06311	0.0118	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant W107A, 7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin
carboxylesterase E93variant W107A	Altererythrobacter indicus	p-nitrophenyl hexanoate	—	0.21	—	pH 7.5	Open record: carboxylesterase E93 variant W107A, p-nitrophenyl hexanoate
carboxylesterase E93variant Y318A	Altererythrobacter indicus	7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin	3.377 × 10⁻⁴	0.07361	0.0047	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant Y318A, 7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin
carboxylesterase E93variant Y318A	Altererythrobacter indicus	7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin	6.812 × 10⁻⁴	0.05547	0.0123	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant Y318A, 7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin
carboxylesterase E93variant Y318A	Altererythrobacter indicus	p-nitrophenyl hexanoate	—	0.18	—	pH 7.5	Open record: carboxylesterase E93 variant Y318A, p-nitrophenyl hexanoate
carboxylesterase E93variant Y318F	Altererythrobacter indicus	7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin	3.67 × 10⁻⁴	0.07346	0.005	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant Y318F, 7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin
carboxylesterase E93variant Y318F	Altererythrobacter indicus	7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin	5.253 × 10⁻⁴	0.07662	0.0068	pH 7.4, 37 °C	Open record: carboxylesterase E93 variant Y318F, 7-ethyl-10-[4-amino-1-piperidinyl] carbonyl oxycamptothecin
carboxylesterase E93variant Y318F	Altererythrobacter indicus	p-nitrophenyl hexanoate	—	0.19	—	pH 7.5	Open record: carboxylesterase E93 variant Y318F, p-nitrophenyl hexanoate