Values CatLog read from this paper
Allosteric modulation of the Lon protease via ssDNA binding and local charge changes
PMID 39542252doi:10.1016/j.jbc.2024.107993Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (13 values) from this paper: Lon protease from Caulobacter crescentus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Lon protease | Caulobacter crescentus | ATPKm from a Hill fit, n = 1.2 | — | 0.092 | — | pH 8, 30 °C | Open record: Lon protease, ATP |
| Lon protease | Caulobacter crescentus | ATPkcat from a Hill fit, n = 1.2; kcat per hexamer | 1.47 | — | — | pH 8, 30 °C | Open record: Lon protease, ATP |
| Lon protease | Caulobacter crescentus | ATPwith 20 µM ssDNA (36-mer oligonucleotide OPC698) | — | 0.054 | — | pH 8, 30 °C | Open record: Lon protease, ATP |
| Lon protease | Caulobacter crescentus | ATPwith 20 µM ssDNA (36-mer oligonucleotide OPC698) | 3.77 | — | — | pH 8, 30 °C | Open record: Lon protease, ATP |
| Lon protease | Caulobacter crescentus | fluorescein isothiocyanate-labeled casein (FITC-casein Type II)Km from a Hill fit, n = 1.1 | — | 7 × 10⁻⁴ | — | pH 8, 30 °C | Open record: Lon protease, fluorescein isothiocyanate-labeled casein (FITC-casein Type II) |
| Lon protease | Caulobacter crescentus | fluorescein isothiocyanate-labeled casein (FITC-casein Type II)kcat from a Hill fit, n = 1.1; kcat per hexamer | 0.033 | — | — | pH 8, 30 °C | Open record: Lon protease, fluorescein isothiocyanate-labeled casein (FITC-casein Type II) |
| Lon protease | Caulobacter crescentus | fluorescein isothiocyanate-labeled casein (FITC-casein Type II)with 20 µM ssDNA (36-mer oligonucleotide OPC698) | — | 6 × 10⁻⁴ | — | pH 8, 30 °C | Open record: Lon protease, fluorescein isothiocyanate-labeled casein (FITC-casein Type II) |
| Lon protease | Caulobacter crescentus | fluorescein isothiocyanate-labeled casein (FITC-casein Type II)with 20 µM ssDNA (36-mer oligonucleotide OPC698) | 0.053 | — | — | pH 8, 30 °C | Open record: Lon protease, fluorescein isothiocyanate-labeled casein (FITC-casein Type II) |
| Lon proteasevariant K301A/K303A/K305A/K306A (Lon4A) | Caulobacter crescentus | ATP | 0.75 | 0.206 | 3.6 calc. | pH 8, 30 °C | Open record: Lon protease variant K301A/K303A/K305A/K306A (Lon4A), ATP |
| Lon proteasevariant K301A/K303A/K305A/K306A (Lon4A) | Caulobacter crescentus | fluorescein isothiocyanate-labeled casein (FITC-casein Type II) | 0.0033 | — | — | pH 8, 30 °C | Open record: Lon protease variant K301A/K303A/K305A/K306A (Lon4A), fluorescein isothiocyanate-labeled casein (FITC-casein Type II) |
| Lon proteasevariant K301E/K303E/K305E/K306E (Lon4E) | Caulobacter crescentus | ATP | 4.53 | — | — | pH 8, 30 °C | Open record: Lon protease variant K301E/K303E/K305E/K306E (Lon4E), ATP |
| Lon proteasevariant K301E/K303E/K305E/K306E (Lon4E) | Caulobacter crescentus | fluorescein isothiocyanate-labeled casein (FITC-casein Type II) | 0.067 | — | — | pH 8, 30 °C | Open record: Lon protease variant K301E/K303E/K305E/K306E (Lon4E), fluorescein isothiocyanate-labeled casein (FITC-casein Type II) |