Values CatLog read from this paper
The NADH-dependent flavin reductase ThdF follows an ordered sequential mechanism though crystal structures reveal two FAD molecules in the active site
PMID 39725031doi:10.1016/j.jbc.2024.108128Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (11 values) from this paper: flavin reductase ThdF from Streptomyces albogriseolus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| flavin reductase ThdF | Streptomyces albogriseolus | flavin adenine dinucleotideNADH fixed at 100 μM; Michaelis–Menten fit | 0.21 | 3.4 × 10⁻⁴ | 620 calc. | 25 °C | Open record: flavin reductase ThdF, flavin adenine dinucleotide |
| flavin reductase ThdF | Streptomyces albogriseolus | flavin adenine dinucleotideNADH fixed at 100 μM | — | 3.4 × 10⁻⁴ | — | 25 °C | Open record: flavin reductase ThdF, flavin adenine dinucleotide |
| flavin reductase ThdF | Streptomyces albogriseolus | flavin adenine dinucleotideFAD variable; NADH fixed | — | — | 620 | 25 °C | Open record: flavin reductase ThdF, flavin adenine dinucleotide |
| flavin reductase ThdF | Streptomyces albogriseolus | flavin mononucleotideMichaelis–Menten fit | 0.2 | 2.8 × 10⁻⁴ | 710 calc. | 25 °C | Open record: flavin reductase ThdF, flavin mononucleotide |
| flavin reductase ThdF | Streptomyces albogriseolus | flavin mononucleotide | — | 2.9 × 10⁻⁴ | — | 25 °C | Open record: flavin reductase ThdF, flavin mononucleotide |
| flavin reductase ThdF | Streptomyces albogriseolus | NADHFAD fixed at 10 μM; Michaelis–Menten fit | 0.26 | 0.0195 | 13 calc. | 25 °C | Open record: flavin reductase ThdF, NADH |
| flavin reductase ThdF | Streptomyces albogriseolus | NADHFAD fixed at 10 μM | — | 0.0108 | — | 25 °C | Open record: flavin reductase ThdF, NADH |
| flavin reductase ThdF | Streptomyces albogriseolus | NADHNADH variable; FAD fixed | — | — | 13 | 25 °C | Open record: flavin reductase ThdF, NADH |