Values CatLog read from this paper
Interplay between conformational dynamics and substrate binding regulates enzymatic activity: a single-molecule FRET study
PMID 39877815doi:10.1039/d4sc06819jOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (12 values) from this paper: adenylate kinase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| adenylate kinasevariant A73C/V142C (dye-labeled; paper's WT) | Escherichia coli | adenosine 5'-monophosphate | 2.62 × 10³ | — | — | pH 8 | Open record: adenylate kinase variant A73C/V142C (dye-labeled; paper's WT), adenosine 5'-monophosphate |
| adenylate kinasevariant A73C/V142C/F86W (dye-labeled) | Escherichia coli | adenosine 5'-monophosphate | 550 | — | — | pH 8 | Open record: adenylate kinase variant A73C/V142C/F86W (dye-labeled), adenosine 5'-monophosphate |
| adenylate kinasevariant C77S/A73C/V142C (WT) | Escherichia coli | adenosine 5'-diphosphate0 M urea | 620 | 1.34 | 460 calc. | pH 8 | Open record: adenylate kinase variant C77S/A73C/V142C (WT), adenosine 5'-diphosphate |
| adenylate kinasevariant C77S/A73C/V142C (WT) | Escherichia coli | adenosine 5'-diphosphate0.2 M urea | 578 | 1.144 | 505 calc. | pH 8 | Open record: adenylate kinase variant C77S/A73C/V142C (WT), adenosine 5'-diphosphate |
| adenylate kinasevariant C77S/A73C/V142C (WT) | Escherichia coli | adenosine 5'-diphosphate0.4 M urea | 590 | 1.064 | 550 calc. | pH 8 | Open record: adenylate kinase variant C77S/A73C/V142C (WT), adenosine 5'-diphosphate |
| adenylate kinasevariant C77S/A73C/V142C (WT) | Escherichia coli | adenosine 5'-diphosphate0.6 M urea | 621 | 1.226 | 507 calc. | pH 8 | Open record: adenylate kinase variant C77S/A73C/V142C (WT), adenosine 5'-diphosphate |
| adenylate kinasevariant C77S/A73C/V142C (WT) | Escherichia coli | adenosine 5'-diphosphate0.8 M urea | 584 | 1.03 | 567 calc. | pH 8 | Open record: adenylate kinase variant C77S/A73C/V142C (WT), adenosine 5'-diphosphate |