Values CatLog read from this paper
Automated Engineering Protein Dynamics via Loop Grafting: Improving Renilla Luciferase Catalysis
PMID 40013243doi:10.1021/acscatal.4c06207Open in Browse
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CatLog read 3 records (9 values) from this paper: Anc(HLD-RLuc) ancestral bifunctional haloalkane dehalogenase/Renilla luciferase, luciferase activity.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Anc(HLD-RLuc) ancestral bifunctional haloalkane dehalogenase/Renilla luciferase, luciferase activityAncFT15 (loop-grafted chimera: loop L9 insert i5, residues 138-162, plus loop L14 insert i9, residues 219-233, of Renilla luciferase RLuc8, replacing the corresponding regions of Anc(HLD-RLuc)) | — | coelenterazine | 0.31 | 3.9 × 10⁻⁴ | 800 | pH 7.5, 37 °C | Open record: Anc(HLD-RLuc) ancestral bifunctional haloalkane dehalogenase/Renilla luciferase, luciferase activity AncFT15 (loop-grafted chimera: loop L9 insert i5, residues 138-162, plus loop L14 insert i9, residues 219-233, of Renilla luciferase RLuc8, replacing the corresponding regions of Anc(HLD-RLuc)), coelenterazine |
| Anc(HLD-RLuc) ancestral bifunctional haloalkane dehalogenase/Renilla luciferase, luciferase activityAncFT16 (loop-grafted chimera: loop L9 insert i1, residues 138-166, plus loop L14 insert i8, residues 219-234, of Renilla luciferase RLuc8, replacing the corresponding regions of Anc(HLD-RLuc)) | — | coelenterazine | 0.13 | 6 × 10⁻⁵ | 2.28 × 10³ | pH 7.5, 37 °C | Open record: Anc(HLD-RLuc) ancestral bifunctional haloalkane dehalogenase/Renilla luciferase, luciferase activity AncFT16 (loop-grafted chimera: loop L9 insert i1, residues 138-166, plus loop L14 insert i8, residues 219-234, of Renilla luciferase RLuc8, replacing the corresponding regions of Anc(HLD-RLuc)), coelenterazine |
| Anc(HLD-RLuc) ancestral bifunctional haloalkane dehalogenase/Renilla luciferase, luciferase activityAncFT9 (loop-grafted chimera: loop L9 insert i2, residues 139-163 of Renilla luciferase RLuc8, replacing the corresponding region of Anc(HLD-RLuc)) | — | coelenterazine | 0.13 | 3.5 × 10⁻⁴ | 370 | pH 7.5, 37 °C | Open record: Anc(HLD-RLuc) ancestral bifunctional haloalkane dehalogenase/Renilla luciferase, luciferase activity AncFT9 (loop-grafted chimera: loop L9 insert i2, residues 139-163 of Renilla luciferase RLuc8, replacing the corresponding region of Anc(HLD-RLuc)), coelenterazine |