Values CatLog read from this paper
Enhanced thermostability of nattokinase by rational design of disulfide bond
PMID 40033318doi:10.1186/s12934-025-02681-5Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 4 records (8 values) from this paper: nattokinase (rAprY) from Bacillus mojavensis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| nattokinase (rAprY) | Bacillus mojavensis | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 3.75 | 1.52 | 2.47 calc. | 25 °C | Open record: nattokinase (rAprY), succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| nattokinase (rAprY)variant A15C/Q271C (M2) | Bacillus mojavensis | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 1.14 | 0.61 | 1.9 calc. | 25 °C | Open record: nattokinase (rAprY) variant A15C/Q271C (M2), succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| nattokinase (rAprY)variant F50C/N109C (M1) | Bacillus mojavensis | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 2.29 | 1.24 | 1.85 calc. | 25 °C | Open record: nattokinase (rAprY) variant F50C/N109C (M1), succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| nattokinase (rAprY)variant F50C/N109C/A15C/Q271C (SS) | Bacillus mojavensis | succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | 1.11 | 0.59 | 1.9 calc. | 25 °C | Open record: nattokinase (rAprY) variant F50C/N109C/A15C/Q271C (SS), succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |