CatLogenzyme kinetics catalog

Values CatLog read from this paper

A steric gate prevents mutagenic dATP incorporation opposite 8-oxo-deoxyguanosine in mitochondrial DNA polymerases

PMID 40070220doi:10.1111/febs.70064Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 30 records (60 values) from this paper: mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1) from Saccharomyces cerevisiae.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 30 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A (Wild-type exo-)Saccharomyces cerevisiae2'-deoxyadenosine 5'-triphosphateG (Table 2 Template column); G (undamaged)0.00380.002141.8 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A (Wild-type exo-)Saccharomyces cerevisiae2'-deoxyadenosine 5'-triphosphate8‐oxodG (Table 2 Template column); 8-oxodG0.01570.00632.5 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A (Wild-type exo-)Saccharomyces cerevisiae2'-deoxycytidine 5'-triphosphateG (Table 2 Template column); G (undamaged)0.05731.67 × 10⁻⁵3.43 × 10³ calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A (Wild-type exo-)Saccharomyces cerevisiae2'-deoxycytidine 5'-triphosphate8‐oxodG (Table 2 Template column); 8-oxodG0.03550.002514 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A (Wild-type exo-)Saccharomyces cerevisiae2'-deoxyguanosine 5'-triphosphateC opposite template G (G:C)0.05181.58 × 10⁻⁵3.28 × 10³ calc.pH 8, 37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A (Wild-type exo-)Saccharomyces cerevisiae2'-deoxyguanosine 5'-triphosphaterow label printed "5‐C 3‐G C‐5"; the same as the row above0.02450.015131.62 calc.pH 8, 37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A (Wild-type exo-)Saccharomyces cerevisiae2'-deoxyguanosine 5'-triphosphateC opposite template 8-oxodG (8-oxodG:C)0.06520.0016838.8 calc.pH 8, 37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A (Wild-type exo-)Saccharomyces cerevisiae2'-deoxyguanosine 5'-triphosphateA opposite template 8-oxodG (8-oxodG:A)0.0429.5 × 10⁻⁴44 calc.pH 8, 37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/F763A (F763A exo-)Saccharomyces cerevisiae2'-deoxyadenosine 5'-triphosphate0.0010150.0018840.5390 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/F763A (F763A exo-)Saccharomyces cerevisiae2'-deoxycytidine 5'-triphosphate0.0014080.002290.615 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/F763A (F763A exo-)Saccharomyces cerevisiae2'-deoxyguanosine 5'-triphosphaterow label printed "5‐A 3‐8oG C‐5"0.00272.1 × 10⁻⁴13 calc.pH 8, 37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/F763A (F763A exo-)Saccharomyces cerevisiae2'-deoxyguanosine 5'-triphosphateA opposite template G (G:A mismatch)9 × 10⁻⁴0.02130.042 calc.pH 8, 37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/F763A (F763A exo-)Saccharomyces cerevisiae2'-deoxyguanosine 5'-triphosphateC opposite template 8-oxodG (8-oxodG:C)0.002080.00141.5 calc.pH 8, 37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/F763A (F763A exo-)Saccharomyces cerevisiae2'-deoxyguanosine 5'-triphosphateA opposite template 8-oxodG (8-oxodG:A)0.00180.0011.8 calc.pH 8, 37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/F763H (F763H exo-)Saccharomyces cerevisiae2'-deoxyadenosine 5'-triphosphate0.0030.00520.58 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/F763H (F763H exo-)Saccharomyces cerevisiae2'-deoxycytidine 5'-triphosphate0.00550.00242.3 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/F763K (F763K exo-)Saccharomyces cerevisiae2'-deoxyadenosine 5'-triphosphate2.2 × 10⁻⁴0.00230.094 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/F763K (F763K exo-)Saccharomyces cerevisiae2'-deoxycytidine 5'-triphosphate2.3 × 10⁻⁴0.00210.11 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/F763L (F763L exo-)Saccharomyces cerevisiae2'-deoxyadenosine 5'-triphosphate0.001020.00760.13 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/F763L (F763L exo-)Saccharomyces cerevisiae2'-deoxycytidine 5'-triphosphate0.00150.00410.37 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/F763Y (F763Y exo-)Saccharomyces cerevisiae2'-deoxyadenosine 5'-triphosphate0.00530.00720.74 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/F763Y (F763Y exo-)Saccharomyces cerevisiae2'-deoxycytidine 5'-triphosphate0.00750.00362.1 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/Y757C (Y757C exo-)Saccharomyces cerevisiae2'-deoxyadenosine 5'-triphosphateG (Table 2 Template column); G (undamaged)9.15 × 10⁻⁴0.02550.0359 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/Y757C (Y757C exo-)Saccharomyces cerevisiae2'-deoxyadenosine 5'-triphosphate8‐oxodG (Table 2 Template column); 8-oxodG2.7 × 10⁻⁴0.0340.0078 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/Y757C (Y757C exo-)Saccharomyces cerevisiae2'-deoxycytidine 5'-triphosphateG (Table 2 Template column); G (undamaged)0.003080.0012892.39 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/Y757C (Y757C exo-)Saccharomyces cerevisiae2'-deoxycytidine 5'-triphosphate8‐oxodG (Table 2 Template column); 8-oxodG4.7 × 10⁻⁴0.02710.017 calc.37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/Y757C (Y757C exo-)Saccharomyces cerevisiae2'-deoxyguanosine 5'-triphosphateG:C primer terminus (Table 3 Template column "5‐C 3‐G C‐5")0.0060.002372.5 calc.pH 8, 37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/Y757C (Y757C exo-)Saccharomyces cerevisiae2'-deoxyguanosine 5'-triphosphateA opposite template G (G:A mismatch)8.8 × 10⁻⁴0.061970.014 calc.pH 8, 37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/Y757C (Y757C exo-)Saccharomyces cerevisiae2'-deoxyguanosine 5'-triphosphateC opposite template 8-oxodG (8-oxodG:C)0.001380.0040.35 calc.pH 8, 37 °COpen
mitochondrial DNA polymerase Mip1 (DNA-directed DNA polymerase gamma MIP1)variant D171A/E173A/Y757C (Y757C exo-)Saccharomyces cerevisiae2'-deoxyguanosine 5'-triphosphateA opposite template 8-oxodG (8-oxodG:A)6.5 × 10⁻⁴0.001960.33 calc.pH 8, 37 °COpen