Values CatLog read from this paper
Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets
PMID 40127491doi:10.1021/jacs.4c11804Open in Browse
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CatLog read 6 records (18 values) from this paper: AxyAgu115A (GH115 glucuronoxylan alpha-glucuronidase) from Amphibacillus xylanus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| AxyAgu115A (GH115 glucuronoxylan alpha-glucuronidase) | Amphibacillus xylanus | beechwood xylan ((4-OMe)GlcA epitope) | 10.97 | 1.49 | 7.36 | Open record: AxyAgu115A (GH115 glucuronoxylan alpha-glucuronidase), beechwood xylan ((4-OMe)GlcA epitope) | |
| AxyAgu115A (GH115 glucuronoxylan alpha-glucuronidase)variant D197E/R259S/I326V/I368N/W430R/G440S (variant D) | Amphibacillus xylanus | beechwood xylan ((4-OMe)GlcA epitope) | 22.68 | 1.66 | 13.7 | Open record: AxyAgu115A (GH115 glucuronoxylan alpha-glucuronidase) variant D197E/R259S/I326V/I368N/W430R/G440S (variant D), beechwood xylan ((4-OMe)GlcA epitope) | |
| AxyAgu115A (GH115 glucuronoxylan alpha-glucuronidase)variant E253D (variant C) | Amphibacillus xylanus | beechwood xylan ((4-OMe)GlcA epitope) | 3.84 | 2 | 1.92 | Open record: AxyAgu115A (GH115 glucuronoxylan alpha-glucuronidase) variant E253D (variant C), beechwood xylan ((4-OMe)GlcA epitope) | |
| AxyAgu115A (GH115 glucuronoxylan alpha-glucuronidase)variant F358L (variant E) | Amphibacillus xylanus | beechwood xylan ((4-OMe)GlcA epitope) | 3.43 | 2 | 1.715 | Open record: AxyAgu115A (GH115 glucuronoxylan alpha-glucuronidase) variant F358L (variant E), beechwood xylan ((4-OMe)GlcA epitope) | |
| AxyAgu115A (GH115 glucuronoxylan alpha-glucuronidase)variant L322Q/Y437F (variant A) | Amphibacillus xylanus | beechwood xylan ((4-OMe)GlcA epitope) | 13.02 | 2.98 | 4.4 | Open record: AxyAgu115A (GH115 glucuronoxylan alpha-glucuronidase) variant L322Q/Y437F (variant A), beechwood xylan ((4-OMe)GlcA epitope) | |
| AxyAgu115A (GH115 glucuronoxylan alpha-glucuronidase)variant L426P (variant B) | Amphibacillus xylanus | beechwood xylan ((4-OMe)GlcA epitope) | 3.11 | 0.55 | 5.65 | Open record: AxyAgu115A (GH115 glucuronoxylan alpha-glucuronidase) variant L426P (variant B), beechwood xylan ((4-OMe)GlcA epitope) |