Values CatLog read from this paper

Engineering mouse chymotrypsin B1 for improved trypsinogen degradation

PMID 40133496doi:10.1038/s41598-025-94299-1Open in Browse

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CatLog read 6 records (18 values) from this paper: chymotrypsin B1 (CTRB1) from Mus musculus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.4.21.1

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
chymotrypsin B1 (CTRB1)variant A244G/Y164A	Mus musculus	Suc-Ala-Ala-Pro-Phe-p-nitroanilide	21.4	0.0741	290	pH 8, 22 °C	Open record: chymotrypsin B1 (CTRB1) variant A244G/Y164A, Suc-Ala-Ala-Pro-Phe-p-nitroanilide
chymotrypsin B1 (CTRB1)variant G236R/A244G/Y164A	Mus musculus	Suc-Ala-Ala-Pro-Phe-p-nitroanilide	24.1	0.0265	910	pH 8, 22 °C	Open record: chymotrypsin B1 (CTRB1) variant G236R/A244G/Y164A, Suc-Ala-Ala-Pro-Phe-p-nitroanilide
chymotrypsin B1 (CTRB1)variant G236R/Y164A	Mus musculus	H-Ala-Ala-Pro-Phe-p-nitroanilide	16	0.178	90	pH 8, 22 °C	Open record: chymotrypsin B1 (CTRB1) variant G236R/Y164A, H-Ala-Ala-Pro-Phe-p-nitroanilide
chymotrypsin B1 (CTRB1)variant G236R/Y164A	Mus musculus	Suc-Ala-Ala-Pro-Phe-p-nitroanilide	26.1	0.0093	2.8 × 10³	pH 8, 22 °C	Open record: chymotrypsin B1 (CTRB1) variant G236R/Y164A, Suc-Ala-Ala-Pro-Phe-p-nitroanilide
chymotrypsin B1 (CTRB1)variant Y164A	Mus musculus	H-Ala-Ala-Pro-Phe-p-nitroanilide	31.1	0.1627	190	pH 8, 22 °C	Open record: chymotrypsin B1 (CTRB1) variant Y164A, H-Ala-Ala-Pro-Phe-p-nitroanilide
chymotrypsin B1 (CTRB1)variant Y164A	Mus musculus	Suc-Ala-Ala-Pro-Phe-p-nitroanilide	38.4	0.0308	1.2 × 10³	pH 8, 22 °C	Open record: chymotrypsin B1 (CTRB1) variant Y164A, Suc-Ala-Ala-Pro-Phe-p-nitroanilide