Values CatLog read from this paper
Characterisation of a cold-adapted, thermostable glucokinase from psychrophilic Pseudoalteromonas sp. AS-131 reveals how the enzyme achieves high thermal stability without loss of cold adaptation
PMID 41397938doi:10.1111/febs.70367Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 30 records (54 values) from this paper: glucokinase (EcGK), glucokinase (PsGK) from Escherichia coli, Pseudoalteromonas sp. AS-131.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glucokinase (EcGK) | Escherichia coli | adenosine 5'-triphosphate | — | 0.78 | — | pH 7.6, 1 °C | Open record: glucokinase (EcGK), adenosine 5'-triphosphate |
| glucokinase (EcGK) | Escherichia coli | adenosine 5'-triphosphate | — | 1.11 | — | pH 7.6, 25 °C | Open record: glucokinase (EcGK), adenosine 5'-triphosphate |
| glucokinase (EcGK) | Escherichia coli | adenosine 5'-triphosphate | — | 2.25 | — | pH 7.6, 40 °C | Open record: glucokinase (EcGK), adenosine 5'-triphosphate |
| glucokinase (EcGK) | Escherichia coli | D-glucoseLineweaver-Burk fit | 16.75 | 1.24 | 13.57 | pH 7.6, 1 °C | Open record: glucokinase (EcGK), D-glucose |
| glucokinase (EcGK) | Escherichia coli | D-glucosecoupled assay: 50 µM glucose-6-phosphate dehydrogenase | — | — | 13.57 | pH 7.6, 1 °C | Open record: glucokinase (EcGK), D-glucose |
| glucokinase (EcGK) | Escherichia coli | D-glucoseLineweaver-Burk fit | 51.36 | 0.73 | 70.41 | pH 7.6, 25 °C | Open record: glucokinase (EcGK), D-glucose |
| glucokinase (EcGK) | Escherichia coli | D-glucosecoupled assay: 50 µM glucose-6-phosphate dehydrogenase | — | — | 70.41 | pH 7.6, 25 °C | Open record: glucokinase (EcGK), D-glucose |
| glucokinase (EcGK) | Escherichia coli | D-glucoseLineweaver-Burk fit | 70.42 | 0.81 | 88.18 | pH 7.6, 40 °C | Open record: glucokinase (EcGK), D-glucose |
| glucokinase (EcGK) | Escherichia coli | D-glucosecoupled assay: 50 µM glucose-6-phosphate dehydrogenase | — | — | 88.18 | pH 7.6, 40 °C | Open record: glucokinase (EcGK), D-glucose |
| glucokinase (EcGK)variant C20S/C65S/S309C | Escherichia coli | adenosine 5'-triphosphate | — | 0.42 | — | pH 7.6, 1 °C | Open record: glucokinase (EcGK) variant C20S/C65S/S309C, adenosine 5'-triphosphate |
| glucokinase (EcGK)variant C20S/C65S/S309C | Escherichia coli | adenosine 5'-triphosphate | — | 0.78 | — | pH 7.6, 25 °C | Open record: glucokinase (EcGK) variant C20S/C65S/S309C, adenosine 5'-triphosphate |
| glucokinase (EcGK)variant C20S/C65S/S309C | Escherichia coli | adenosine 5'-triphosphate | — | 1.46 | — | pH 7.6, 40 °C | Open record: glucokinase (EcGK) variant C20S/C65S/S309C, adenosine 5'-triphosphate |
| glucokinase (EcGK)variant C20S/C65S/S309C | Escherichia coli | D-glucose | 23.45 | 0.83 | 28.29 | pH 7.6, 1 °C | Open record: glucokinase (EcGK) variant C20S/C65S/S309C, D-glucose |
| glucokinase (EcGK)variant C20S/C65S/S309C | Escherichia coli | D-glucose | 42.94 | 0.37 | 116.7 | pH 7.6, 25 °C | Open record: glucokinase (EcGK) variant C20S/C65S/S309C, D-glucose |
| glucokinase (EcGK)variant C20S/C65S/S309C | Escherichia coli | D-glucose | 63.08 | 0.91 | 69.12 | pH 7.6, 40 °C | Open record: glucokinase (EcGK) variant C20S/C65S/S309C, D-glucose |
| glucokinase (PsGK) | Pseudoalteromonas sp. AS-131 | adenosine 5'-triphosphate | — | 0.83 | — | pH 7.6, 1 °C | Open record: glucokinase (PsGK), adenosine 5'-triphosphate |
| glucokinase (PsGK) | Pseudoalteromonas sp. AS-131 | adenosine 5'-triphosphate | — | 1.55 | — | pH 7.6, 25 °C | Open record: glucokinase (PsGK), adenosine 5'-triphosphate |
| glucokinase (PsGK) | Pseudoalteromonas sp. AS-131 | adenosine 5'-triphosphate | — | 1.54 | — | pH 7.6, 40 °C | Open record: glucokinase (PsGK), adenosine 5'-triphosphate |
| glucokinase (PsGK) | Pseudoalteromonas sp. AS-131 | D-glucoseLineweaver-Burk fit | 11.25 | 0.12 | 92.15 | pH 7.6, 1 °C | Open record: glucokinase (PsGK), D-glucose |
| glucokinase (PsGK) | Pseudoalteromonas sp. AS-131 | D-glucosecoupled assay: 50 µM glucose-6-phosphate dehydrogenase | — | — | 92.15 | pH 7.6, 1 °C | Open record: glucokinase (PsGK), D-glucose |
| glucokinase (PsGK) | Pseudoalteromonas sp. AS-131 | D-glucoseLineweaver-Burk fit | 33.49 | 0.36 | 93.99 | pH 7.6, 25 °C | Open record: glucokinase (PsGK), D-glucose |
| glucokinase (PsGK) | Pseudoalteromonas sp. AS-131 | D-glucosecoupled assay: 50 µM glucose-6-phosphate dehydrogenase | — | — | 93.99 | pH 7.6, 25 °C | Open record: glucokinase (PsGK), D-glucose |
| glucokinase (PsGK) | Pseudoalteromonas sp. AS-131 | D-glucoseLineweaver-Burk fit | 40.01 | 0.6 | 67.93 | pH 7.6, 40 °C | Open record: glucokinase (PsGK), D-glucose |
| glucokinase (PsGK) | Pseudoalteromonas sp. AS-131 | D-glucosecoupled assay: 50 µM glucose-6-phosphate dehydrogenase | — | — | 67.93 | pH 7.6, 40 °C | Open record: glucokinase (PsGK), D-glucose |
| glucokinase (PsGK)variant C325S | Pseudoalteromonas sp. AS-131 | adenosine 5'-triphosphate | — | 0.52 | — | pH 7.6, 1 °C | Open record: glucokinase (PsGK) variant C325S, adenosine 5'-triphosphate |
| glucokinase (PsGK)variant C325S | Pseudoalteromonas sp. AS-131 | adenosine 5'-triphosphate | — | 0.96 | — | pH 7.6, 25 °C | Open record: glucokinase (PsGK) variant C325S, adenosine 5'-triphosphate |
| glucokinase (PsGK)variant C325S | Pseudoalteromonas sp. AS-131 | adenosine 5'-triphosphate | — | 1.86 | — | pH 7.6, 40 °C | Open record: glucokinase (PsGK) variant C325S, adenosine 5'-triphosphate |
| glucokinase (PsGK)variant C325S | Pseudoalteromonas sp. AS-131 | D-glucose | 15.74 | 0.1 | 159.9 | pH 7.6, 1 °C | Open record: glucokinase (PsGK) variant C325S, D-glucose |
| glucokinase (PsGK)variant C325S | Pseudoalteromonas sp. AS-131 | D-glucose | 48.57 | 0.21 | 237.8 | pH 7.6, 25 °C | Open record: glucokinase (PsGK) variant C325S, D-glucose |
| glucokinase (PsGK)variant C325S | Pseudoalteromonas sp. AS-131 | D-glucose | 100.4 | 0.29 | 248.0 | pH 7.6, 40 °C | Open record: glucokinase (PsGK) variant C325S, D-glucose |