Values CatLog read from this paper
De novo cysteine biosynthesis in Pseudomonas aeruginosa: Characterization of the two main cysteine synthase isoforms
PMID 41541697doi:10.1016/j.isci.2025.114304Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (32 values) from this paper: cysteine synthase B, CysM (PA0932), cysteine synthase B, PaCysM (PA0932), cysteine synthase A, CysK (PA2709) and 1 more from Pseudomonas aeruginosa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cysteine synthase A, CysK (PA2709) | Pseudomonas aeruginosa | hydrosulfide | — | — | 3.3 × 10⁴ | pH 7, 25 °C | Open record: cysteine synthase A, CysK (PA2709), hydrosulfide |
| cysteine synthase A, CysK (PA2709) | Pseudomonas aeruginosa | O-acetyl-L-serine | — | — | 90 | pH 7, 25 °C | Open record: cysteine synthase A, CysK (PA2709), O-acetyl-L-serine |
| cysteine synthase A, PaCysK (PA2709) | Pseudomonas aeruginosa | hydrogensulfide | 100 | 0.0031 | 3.3 × 10⁴ | pH 7, 25 °C | Open record: cysteine synthase A, PaCysK (PA2709), hydrogensulfide |
| cysteine synthase A, PaCysK (PA2709) | Pseudomonas aeruginosa | O-acetyl-L-serine | 102 | 1.1 | 90 | pH 7, 25 °C | Open record: cysteine synthase A, PaCysK (PA2709), O-acetyl-L-serine |
| cysteine synthase B, CysM (PA0932) | Pseudomonas aeruginosa | hydrosulfidebisulfide varied at 5; 10 and 30 mM OAS | — | — | 1.45 × 10⁴ | pH 7, 25 °C | Open record: cysteine synthase B, CysM (PA0932), hydrosulfide |
| cysteine synthase B, CysM (PA0932) | Pseudomonas aeruginosa | hydrosulfidebisulfide varied at 10 mM OAS | 333 | 0.016 | 2.1 × 10⁴ calc. | pH 7, 25 °C | Open record: cysteine synthase B, CysM (PA0932), hydrosulfide |
| cysteine synthase B, CysM (PA0932) | Pseudomonas aeruginosa | hydrosulfidebisulfide varied at 90 mM OAS | 797 | 0.0563 | 1.42 × 10⁴ calc. | pH 7, 25 °C | Open record: cysteine synthase B, CysM (PA0932), hydrosulfide |
| cysteine synthase B, CysM (PA0932) | Pseudomonas aeruginosa | O-acetyl-L-serinebisulfide varied at 5; 10 and 30 mM OAS | — | — | 89 | pH 7, 25 °C | Open record: cysteine synthase B, CysM (PA0932), O-acetyl-L-serine |
| cysteine synthase B, CysM (PA0932) | Pseudomonas aeruginosa | O-acetyl-L-serine | — | — | 79 | pH 7, 25 °C | Open record: cysteine synthase B, CysM (PA0932), O-acetyl-L-serine |
| cysteine synthase B, CysM (PA0932) | Pseudomonas aeruginosa | O-acetyl-L-serineOAS varied at 160 µM bisulfide | 499 | 9.1 | 55 calc. | pH 7, 25 °C | Open record: cysteine synthase B, CysM (PA0932), O-acetyl-L-serine |
| cysteine synthase B, CysM (PA0932) | Pseudomonas aeruginosa | thiosulfate | — | — | 1.3 × 10³ | pH 7, 25 °C | Open record: cysteine synthase B, CysM (PA0932), thiosulfate |
| cysteine synthase B, PaCysM (PA0932) | Pseudomonas aeruginosa | hydrogensulfidebisulfide varied at 5; 10 and 30 mM OAS | 1069 | 0.074 | 1.45 × 10⁴ | pH 7, 25 °C | Open record: cysteine synthase B, PaCysM (PA0932), hydrogensulfide |
| cysteine synthase B, PaCysM (PA0932) | Pseudomonas aeruginosa | hydrogensulfidesingle-substrate Michaelis-Menten fit (Eq. 3) | — | 0.016 | — | pH 7, 25 °C | Open record: cysteine synthase B, PaCysM (PA0932), hydrogensulfide |
| cysteine synthase B, PaCysM (PA0932) | Pseudomonas aeruginosa | hydrogensulfidebisulfide varied at fixed 90 mM OAS | 797 | 0.056 | 1.4 × 10⁴ calc. | pH 7, 25 °C | Open record: cysteine synthase B, PaCysM (PA0932), hydrogensulfide |
| cysteine synthase B, PaCysM (PA0932) | Pseudomonas aeruginosa | O-acetyl-L-serinebisulfide varied at 5; 10 and 30 mM OAS | — | 12 | 89 | pH 7, 25 °C | Open record: cysteine synthase B, PaCysM (PA0932), O-acetyl-L-serine |
| cysteine synthase B, PaCysM (PA0932) | Pseudomonas aeruginosa | O-acetyl-L-serine | 209 | 2.7 | 79 | pH 7, 25 °C | Open record: cysteine synthase B, PaCysM (PA0932), O-acetyl-L-serine |
| cysteine synthase B, PaCysM (PA0932) | Pseudomonas aeruginosa | O-acetyl-L-serinesingle-substrate Michaelis-Menten fit (Eq. 3) | — | 9 | — | pH 7, 25 °C | Open record: cysteine synthase B, PaCysM (PA0932), O-acetyl-L-serine |
| cysteine synthase B, PaCysM (PA0932) | Pseudomonas aeruginosa | thiosulfate | — | 0.16 | 1.3 × 10³ | pH 7, 25 °C | Open record: cysteine synthase B, PaCysM (PA0932), thiosulfate |