Values CatLog read from this paper
Computational evolution of poly(U) polymerase for efficient and controlled RNA oligonucleotide synthesis
PMID 41603735doi:10.1093/nar/gkaf1529Open in Browse
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CatLog read 32 records (96 values) from this paper: poly(U) polymerase (PUP).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| poly(U) polymerase (PUP) | — | 3′-O-allyladenosine 5′-triphosphate | 0.0058 | 0.0035 | 1.7 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP), 3′-O-allyladenosine 5′-triphosphate |
| poly(U) polymerase (PUP) | — | 3′-O-allylcytidine 5′-triphosphate | 0.0017 | 0.08962 | 0.018 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP), 3′-O-allylcytidine 5′-triphosphate |
| poly(U) polymerase (PUP) | — | 3′-O-allylguanosine 5′-triphosphate | 0.0042 | 0.00664 | 0.63 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP), 3′-O-allylguanosine 5′-triphosphate |
| poly(U) polymerase (PUP) | — | 3′-O-allyluridine 5′-triphosphate | 0.004 | 0.00229 | 1.8 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP), 3′-O-allyluridine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45L/L223Q | — | 3′-O-allyladenosine 5′-triphosphate | 0.0122 | 0.00551 | 2.2 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45L/L223Q, 3′-O-allyladenosine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45L/L223Q | — | 3′-O-allylcytidine 5′-triphosphate | 0.008 | 0.01172 | 0.68 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45L/L223Q, 3′-O-allylcytidine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45L/L223Q | — | 3′-O-allylguanosine 5′-triphosphate | 0.011 | 0.00475 | 2.3 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45L/L223Q, 3′-O-allylguanosine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45L/L223Q | — | 3′-O-allyluridine 5′-triphosphate | 0.0183 | 8.5 × 10⁻⁴ | 22 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45L/L223Q, 3′-O-allyluridine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45P | — | 3′-O-allyladenosine 5′-triphosphate | 0.0158 | 0.00639 | 2.5 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45P, 3′-O-allyladenosine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45P | — | 3′-O-allylcytidine 5′-triphosphate | 0.0075 | 0.00572 | 1.32 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45P, 3′-O-allylcytidine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45P | — | 3′-O-allylguanosine 5′-triphosphate | 0.0095 | 0.00524 | 1.8 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45P, 3′-O-allylguanosine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45P | — | 3′-O-allyluridine 5′-triphosphate | 0.0178 | 9.2 × 10⁻⁴ | 20 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45P, 3′-O-allyluridine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45P/I128V/S165T/L223Q | — | 3′-O-allyladenosine 5′-triphosphate | 0.0112 | 0.0066 | 1.7 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45P/I128V/S165T/L223Q, 3′-O-allyladenosine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45P/I128V/S165T/L223Q | — | 3′-O-allylcytidine 5′-triphosphate | 0.0077 | 0.011 | 0.7 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45P/I128V/S165T/L223Q, 3′-O-allylcytidine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45P/I128V/S165T/L223Q | — | 3′-O-allylguanosine 5′-triphosphate | 0.0127 | 0.00562 | 2.2 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45P/I128V/S165T/L223Q, 3′-O-allylguanosine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45P/I128V/S165T/L223Q | — | 3′-O-allyluridine 5′-triphosphate | 0.0188 | 0.00124 | 15.2 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45P/I128V/S165T/L223Q, 3′-O-allyluridine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45P/L223Q/P273Y | — | 3′-O-allyladenosine 5′-triphosphate | 0.0088 | 0.00528 | 1.7 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45P/L223Q/P273Y, 3′-O-allyladenosine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45P/L223Q/P273Y | — | 3′-O-allylcytidine 5′-triphosphate | 0.0033 | 0.00525 | 0.62 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45P/L223Q/P273Y, 3′-O-allylcytidine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45P/L223Q/P273Y | — | 3′-O-allylguanosine 5′-triphosphate | 0.0128 | 0.00805 | 1.58 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45P/L223Q/P273Y, 3′-O-allylguanosine 5′-triphosphate |
| poly(U) polymerase (PUP)variant I45P/L223Q/P273Y | — | 3′-O-allyluridine 5′-triphosphate | 0.0198 | 0.00106 | 18 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant I45P/L223Q/P273Y, 3′-O-allyluridine 5′-triphosphate |
| poly(U) polymerase (PUP)variant N159F | — | 3′-O-allyladenosine 5′-triphosphate | 0.0088 | 0.00547 | 1.63 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant N159F, 3′-O-allyladenosine 5′-triphosphate |
| poly(U) polymerase (PUP)variant N159F | — | 3′-O-allylcytidine 5′-triphosphate | 0.0072 | 0.01108 | 0.65 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant N159F, 3′-O-allylcytidine 5′-triphosphate |
| poly(U) polymerase (PUP)variant N159F | — | 3′-O-allylguanosine 5′-triphosphate | 0.007 | 0.00134 | 5.2 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant N159F, 3′-O-allylguanosine 5′-triphosphate |
| poly(U) polymerase (PUP)variant N159F | — | 3′-O-allyluridine 5′-triphosphate | 0.011 | 0.00298 | 3.7 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant N159F, 3′-O-allyluridine 5′-triphosphate |
| poly(U) polymerase (PUP)variant PUPdel | — | 3′-O-allyladenosine 5′-triphosphate | 0.0137 | 0.00866 | 1.58 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant PUPdel, 3′-O-allyladenosine 5′-triphosphate |
| poly(U) polymerase (PUP)variant PUPdel | — | 3′-O-allylcytidine 5′-triphosphate | 0.005 | 0.01488 | 0.33 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant PUPdel, 3′-O-allylcytidine 5′-triphosphate |
| poly(U) polymerase (PUP)variant PUPdel | — | 3′-O-allylguanosine 5′-triphosphate | 0.0082 | 0.00717 | 1.15 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant PUPdel, 3′-O-allylguanosine 5′-triphosphate |
| poly(U) polymerase (PUP)variant PUPdel | — | 3′-O-allyluridine 5′-triphosphate | 0.0112 | 7.2 × 10⁻⁴ | 15.5 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant PUPdel, 3′-O-allyluridine 5′-triphosphate |
| poly(U) polymerase (PUP)variant PUPdel2 | — | 3′-O-allyladenosine 5′-triphosphate | 0.0272 | 0.00495 | 5.5 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant PUPdel2, 3′-O-allyladenosine 5′-triphosphate |
| poly(U) polymerase (PUP)variant PUPdel2 | — | 3′-O-allylcytidine 5′-triphosphate | 0.0043 | 0.01158 | 0.38 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant PUPdel2, 3′-O-allylcytidine 5′-triphosphate |
| poly(U) polymerase (PUP)variant PUPdel2 | — | 3′-O-allylguanosine 5′-triphosphate | 0.017 | 0.00998 | 1.7 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant PUPdel2, 3′-O-allylguanosine 5′-triphosphate |
| poly(U) polymerase (PUP)variant PUPdel2 | — | 3′-O-allyluridine 5′-triphosphate | 0.061 | 7.4 × 10⁻⁴ | 83 | pH 7.9, 37 °C | Open record: poly(U) polymerase (PUP) variant PUPdel2, 3′-O-allyluridine 5′-triphosphate |