Values CatLog read from this paper
C2α-carbanion-protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2-hydroxyacyl-CoA lyase
PMID 41606311doi:10.1002/2211-5463.70199Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (27 values) from this paper: actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL) | — | 2-hydroxy-2-methylbutyryl-CoA (racemic mixture) | 0.87 | 0.052 | 17 | pH 7.2, 37 °C | Open record: actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL), 2-hydroxy-2-methylbutyryl-CoA (racemic mixture) |
| actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL) | — | Acetol | 6.6 | 37 | 0.18 | pH 7.2, 37 °C | Open record: actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL), Acetol |
| actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL)variant E493A | — | Acetol | 0.35 | 2.3 | 0.15 | pH 7.2, 37 °C | Open record: actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL) variant E493A, Acetol |
| actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL)variant E493D | — | 2-hydroxyisobutyryl-CoA | 0.0024 | 0.123 | 0.02 | pH 7.2, 37 °C | Open record: actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL) variant E493D, 2-hydroxyisobutyryl-CoA |
| actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL)variant E493S | — | 2-hydroxyisobutyryl-CoA | 0.12 | 0.022 | 5.4 | pH 7.2, 37 °C | Open record: actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL) variant E493S, 2-hydroxyisobutyryl-CoA |
| actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL)variant E493S | — | Acetol | 0.52 | 2.6 | 0.2 | pH 7.2, 37 °C | Open record: actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL) variant E493S, Acetol |
| actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL)variant E493S | — | Dihydroxyacetone | 0.63 | 4.4 | 0.14 | pH 7.2, 37 °C | Open record: actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL) variant E493S, Dihydroxyacetone |
| actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL)variant E493S | — | Glycolaldehyde | 0.52 | 0.84 | 0.62 | pH 7.2, 37 °C | Open record: actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL) variant E493S, Glycolaldehyde |
| actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL)variant E493S | — | Pentan-3-one | 0.25 | 4.8 | 0.052 | pH 7.2, 37 °C | Open record: actinobacterial 2-hydroxyacyl-CoA lyase (AcHACL) variant E493S, Pentan-3-one |