Values CatLog read from this paper

Biochemical and structural analyses of metallo-β-lactamase VIM-28: impact of substitutions at residues 224 and 228 on substrate profile, stability, and zinc affinity

PMID 41660846doi:10.1128/spectrum.02788-25Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 96 records (252 values) from this paper: VIM-28, VIM-1, VIM-26 and 1 more from Pseudomonas aeruginosa.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.5.2.6

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
VIM-1	Pseudomonas aeruginosa	Ampicillin	565	0.215	2.63 × 10³	pH 7.5, 30 °C	Open record: VIM-1, Ampicillin
VIM-1	Pseudomonas aeruginosa	benzylpenicillin	752	0.329	2.29 × 10³	pH 7.5, 30 °C	Open record: VIM-1, benzylpenicillin
VIM-1	Pseudomonas aeruginosa	Cefotaxime	588	0.134	4.39 × 10³	pH 7.5, 30 °C	Open record: VIM-1, Cefotaxime
VIM-1	Pseudomonas aeruginosa	Ceftazidime	—	—	168	pH 7.5, 30 °C	Open record: VIM-1, Ceftazidime
VIM-1	Pseudomonas aeruginosa	Cephalothin	663	0.077	8.61 × 10³	pH 7.5, 30 °C	Open record: VIM-1, Cephalothin
VIM-1	Pseudomonas aeruginosa	Meropenem	75.5	0.339	222	pH 7.5, 30 °C	Open record: VIM-1, Meropenem
VIM-26	Pseudomonas aeruginosa	Ampicillin	931	0.513	1.81 × 10³	pH 7.5, 30 °C	Open record: VIM-26, Ampicillin
VIM-26	Pseudomonas aeruginosa	benzylpenicillin	—	—	630	pH 7.5, 30 °C	Open record: VIM-26, benzylpenicillin
VIM-26	Pseudomonas aeruginosa	Cefotaxime	—	—	2.25 × 10³	pH 7.5, 30 °C	Open record: VIM-26, Cefotaxime
VIM-26	Pseudomonas aeruginosa	Ceftazidime	—	—	33.9	pH 7.5, 30 °C	Open record: VIM-26, Ceftazidime
VIM-26	Pseudomonas aeruginosa	Cephalothin	884	0.15	5.89 × 10³	pH 7.5, 30 °C	Open record: VIM-26, Cephalothin
VIM-26	Pseudomonas aeruginosa	Meropenem	90	0.241	393	pH 7.5, 30 °C	Open record: VIM-26, Meropenem
VIM-28	Pseudomonas aeruginosa	Ampicillin	191	0.0125	1.53 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28, Ampicillin
VIM-28	Pseudomonas aeruginosa	benzylpenicillin	1.24 × 10³	0.176	7.03 × 10³	pH 7.5, 30 °C	Open record: VIM-28, benzylpenicillin
VIM-28	Pseudomonas aeruginosa	Cefotaxime	285	0.0272	1.05 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28, Cefotaxime
VIM-28	Pseudomonas aeruginosa	Ceftazidime	—	—	35.9	pH 7.5, 30 °C	Open record: VIM-28, Ceftazidime
VIM-28	Pseudomonas aeruginosa	Cephalothin	276	0.00966	2.86 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28, Cephalothin
VIM-28	Pseudomonas aeruginosa	Meropenem	20.6	0.0143	1.44 × 10³	pH 7.5, 30 °C	Open record: VIM-28, Meropenem
VIM-28variant A148V	Pseudomonas aeruginosa	Ampicillin	186	0.0158	1.18 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant A148V, Ampicillin
VIM-28variant A148V	Pseudomonas aeruginosa	benzylpenicillin	1.23 × 10³	0.171	7.21 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant A148V, benzylpenicillin
VIM-28variant A148V	Pseudomonas aeruginosa	Cefotaxime	355	0.0331	1.07 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant A148V, Cefotaxime
VIM-28variant A148V	Pseudomonas aeruginosa	Ceftazidime	—	—	32.8	pH 7.5, 30 °C	Open record: VIM-28 variant A148V, Ceftazidime
VIM-28variant A148V	Pseudomonas aeruginosa	Cephalothin	292	0.0111	2.62 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant A148V, Cephalothin
VIM-28variant A148V	Pseudomonas aeruginosa	Meropenem	22.8	0.0136	1.68 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant A148V, Meropenem
VIM-28variant A304T	Pseudomonas aeruginosa	Ampicillin	163	0.0164	9.91 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant A304T, Ampicillin
VIM-28variant A304T	Pseudomonas aeruginosa	benzylpenicillin	1.11 × 10³	0.197	5.6 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant A304T, benzylpenicillin
VIM-28variant A304T	Pseudomonas aeruginosa	Cefotaxime	284	0.0276	1.03 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant A304T, Cefotaxime
VIM-28variant A304T	Pseudomonas aeruginosa	Ceftazidime	—	—	27.6	pH 7.5, 30 °C	Open record: VIM-28 variant A304T, Ceftazidime
VIM-28variant A304T	Pseudomonas aeruginosa	Cephalothin	240	0.0103	2.32 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant A304T, Cephalothin
VIM-28variant A304T	Pseudomonas aeruginosa	Meropenem	19.5	0.0127	1.54 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant A304T, Meropenem
VIM-28variant E257Q/V258F	Pseudomonas aeruginosa	Ampicillin	177	0.0171	1.04 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant E257Q/V258F, Ampicillin
VIM-28variant E257Q/V258F	Pseudomonas aeruginosa	benzylpenicillin	1.2 × 10³	0.159	7.56 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant E257Q/V258F, benzylpenicillin
VIM-28variant E257Q/V258F	Pseudomonas aeruginosa	Cefotaxime	302	0.0322	9.41 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant E257Q/V258F, Cefotaxime
VIM-28variant E257Q/V258F	Pseudomonas aeruginosa	Ceftazidime	—	—	33.1	pH 7.5, 30 °C	Open record: VIM-28 variant E257Q/V258F, Ceftazidime
VIM-28variant E257Q/V258F	Pseudomonas aeruginosa	Cephalothin	274	0.0103	2.67 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant E257Q/V258F, Cephalothin
VIM-28variant E257Q/V258F	Pseudomonas aeruginosa	Meropenem	20.1	0.0119	1.69 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant E257Q/V258F, Meropenem
VIM-28variant H24F/G26V/E27D/P28S (N-term)	Pseudomonas aeruginosa	Ampicillin	212	0.0192	1.1 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant H24F/G26V/E27D/P28S (N-term), Ampicillin
VIM-28variant H24F/G26V/E27D/P28S (N-term)	Pseudomonas aeruginosa	benzylpenicillin	1.29 × 10³	0.159	8.11 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant H24F/G26V/E27D/P28S (N-term), benzylpenicillin
VIM-28variant H24F/G26V/E27D/P28S (N-term)	Pseudomonas aeruginosa	Cefotaxime	322	0.0284	1.13 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant H24F/G26V/E27D/P28S (N-term), Cefotaxime
VIM-28variant H24F/G26V/E27D/P28S (N-term)	Pseudomonas aeruginosa	Ceftazidime	—	—	33.7	pH 7.5, 30 °C	Open record: VIM-28 variant H24F/G26V/E27D/P28S (N-term), Ceftazidime
VIM-28variant H24F/G26V/E27D/P28S (N-term)	Pseudomonas aeruginosa	Cephalothin	296	0.011	2.7 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant H24F/G26V/E27D/P28S (N-term), Cephalothin
VIM-28variant H24F/G26V/E27D/P28S (N-term)	Pseudomonas aeruginosa	Meropenem	18.6	0.0131	1.42 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant H24F/G26V/E27D/P28S (N-term), Meropenem
VIM-28variant K251Q	Pseudomonas aeruginosa	Ampicillin	191	0.0169	1.13 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant K251Q, Ampicillin
VIM-28variant K251Q	Pseudomonas aeruginosa	benzylpenicillin	1.37 × 10³	0.199	6.87 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant K251Q, benzylpenicillin
VIM-28variant K251Q	Pseudomonas aeruginosa	Cefotaxime	352	0.0346	1.01 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant K251Q, Cefotaxime
VIM-28variant K251Q	Pseudomonas aeruginosa	Ceftazidime	—	—	31.3	pH 7.5, 30 °C	Open record: VIM-28 variant K251Q, Ceftazidime
VIM-28variant K251Q	Pseudomonas aeruginosa	Cephalothin	289	0.011	2.63 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant K251Q, Cephalothin
VIM-28variant K251Q	Pseudomonas aeruginosa	Meropenem	22.5	0.0144	1.56 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant K251Q, Meropenem
VIM-28variant K311T/A316V (C-term)	Pseudomonas aeruginosa	Ampicillin	245	0.018	1.32 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant K311T/A316V (C-term), Ampicillin
VIM-28variant K311T/A316V (C-term)	Pseudomonas aeruginosa	benzylpenicillin	1.35 × 10³	0.174	7.73 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant K311T/A316V (C-term), benzylpenicillin
VIM-28variant K311T/A316V (C-term)	Pseudomonas aeruginosa	Cefotaxime	400	0.038	1.05 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant K311T/A316V (C-term), Cefotaxime
VIM-28variant K311T/A316V (C-term)	Pseudomonas aeruginosa	Ceftazidime	—	—	31	pH 7.5, 30 °C	Open record: VIM-28 variant K311T/A316V (C-term), Ceftazidime
VIM-28variant K311T/A316V (C-term)	Pseudomonas aeruginosa	Cephalothin	363	0.0112	3.23 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant K311T/A316V (C-term), Cephalothin
VIM-28variant K311T/A316V (C-term)	Pseudomonas aeruginosa	Meropenem	22.5	0.0141	1.6 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant K311T/A316V (C-term), Meropenem
VIM-28variant N215S/E257Q/V258F	Pseudomonas aeruginosa	Ampicillin	173	0.014	1.24 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant N215S/E257Q/V258F, Ampicillin
VIM-28variant N215S/E257Q/V258F	Pseudomonas aeruginosa	benzylpenicillin	1.1 × 10³	0.137	8.02 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant N215S/E257Q/V258F, benzylpenicillin
VIM-28variant N215S/E257Q/V258F	Pseudomonas aeruginosa	Cefotaxime	284	0.0312	9.11 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant N215S/E257Q/V258F, Cefotaxime
VIM-28variant N215S/E257Q/V258F	Pseudomonas aeruginosa	Ceftazidime	—	—	33	pH 7.5, 30 °C	Open record: VIM-28 variant N215S/E257Q/V258F, Ceftazidime
VIM-28variant N215S/E257Q/V258F	Pseudomonas aeruginosa	Cephalothin	219	0.01	2.19 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant N215S/E257Q/V258F, Cephalothin
VIM-28variant N215S/E257Q/V258F	Pseudomonas aeruginosa	Meropenem	18.7	0.0129	1.45 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant N215S/E257Q/V258F, Meropenem
VIM-28variant V223I/L224Y	Pseudomonas aeruginosa	Ampicillin	191	0.0195	9.8 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V223I/L224Y, Ampicillin
VIM-28variant V223I/L224Y	Pseudomonas aeruginosa	benzylpenicillin	567	0.121	4.69 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V223I/L224Y, benzylpenicillin
VIM-28variant V223I/L224Y	Pseudomonas aeruginosa	Cefotaxime	169	0.024	7.05 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V223I/L224Y, Cefotaxime
VIM-28variant V223I/L224Y	Pseudomonas aeruginosa	Ceftazidime	—	—	83.4	pH 7.5, 30 °C	Open record: VIM-28 variant V223I/L224Y, Ceftazidime
VIM-28variant V223I/L224Y	Pseudomonas aeruginosa	Cephalothin	258	0.00773	3.34 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant V223I/L224Y, Cephalothin
VIM-28variant V223I/L224Y	Pseudomonas aeruginosa	Meropenem	9.12	0.00721	1.26 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V223I/L224Y, Meropenem
VIM-28variant V223I/L224Y/V248I	Pseudomonas aeruginosa	Ampicillin	201	0.0224	9 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V223I/L224Y/V248I, Ampicillin
VIM-28variant V223I/L224Y/V248I	Pseudomonas aeruginosa	benzylpenicillin	482	0.118	4.09 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V223I/L224Y/V248I, benzylpenicillin
VIM-28variant V223I/L224Y/V248I	Pseudomonas aeruginosa	Cefotaxime	155	0.0248	6.25 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V223I/L224Y/V248I, Cefotaxime
VIM-28variant V223I/L224Y/V248I	Pseudomonas aeruginosa	Ceftazidime	—	—	96.6	pH 7.5, 30 °C	Open record: VIM-28 variant V223I/L224Y/V248I, Ceftazidime
VIM-28variant V223I/L224Y/V248I	Pseudomonas aeruginosa	Cephalothin	248	0.0078	3.18 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant V223I/L224Y/V248I, Cephalothin
VIM-28variant V223I/L224Y/V248I	Pseudomonas aeruginosa	Meropenem	7.71	0.00817	944	pH 7.5, 30 °C	Open record: VIM-28 variant V223I/L224Y/V248I, Meropenem
VIM-28variant V248I	Pseudomonas aeruginosa	Ampicillin	206	0.0162	1.27 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant V248I, Ampicillin
VIM-28variant V248I	Pseudomonas aeruginosa	benzylpenicillin	1.41 × 10³	0.179	7.89 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V248I, benzylpenicillin
VIM-28variant V248I	Pseudomonas aeruginosa	Cefotaxime	383	0.0314	1.22 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant V248I, Cefotaxime
VIM-28variant V248I	Pseudomonas aeruginosa	Ceftazidime	—	—	36.3	pH 7.5, 30 °C	Open record: VIM-28 variant V248I, Ceftazidime
VIM-28variant V248I	Pseudomonas aeruginosa	Cephalothin	297	0.0098	3.03 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant V248I, Cephalothin
VIM-28variant V248I	Pseudomonas aeruginosa	Meropenem	21.1	0.0136	1.56 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V248I, Meropenem
VIM-28variant V248I/Q301K/A304T	Pseudomonas aeruginosa	Ampicillin	143	0.0157	9.14 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V248I/Q301K/A304T, Ampicillin
VIM-28variant V248I/Q301K/A304T	Pseudomonas aeruginosa	benzylpenicillin	1.07 × 10³	0.166	6.44 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V248I/Q301K/A304T, benzylpenicillin
VIM-28variant V248I/Q301K/A304T	Pseudomonas aeruginosa	Cefotaxime	280	0.0339	8.24 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V248I/Q301K/A304T, Cefotaxime
VIM-28variant V248I/Q301K/A304T	Pseudomonas aeruginosa	Ceftazidime	—	—	26	pH 7.5, 30 °C	Open record: VIM-28 variant V248I/Q301K/A304T, Ceftazidime
VIM-28variant V248I/Q301K/A304T	Pseudomonas aeruginosa	Cephalothin	245	0.0103	2.38 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant V248I/Q301K/A304T, Cephalothin
VIM-28variant V248I/Q301K/A304T	Pseudomonas aeruginosa	Meropenem	16.5	0.0131	1.26 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V248I/Q301K/A304T, Meropenem
VIM-28variant V258F	Pseudomonas aeruginosa	Ampicillin	178	0.0142	1.25 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant V258F, Ampicillin
VIM-28variant V258F	Pseudomonas aeruginosa	benzylpenicillin	1.28 × 10³	0.186	6.93 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V258F, benzylpenicillin
VIM-28variant V258F	Pseudomonas aeruginosa	Cefotaxime	305	0.0305	1 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant V258F, Cefotaxime
VIM-28variant V258F	Pseudomonas aeruginosa	Ceftazidime	—	—	33.3	pH 7.5, 30 °C	Open record: VIM-28 variant V258F, Ceftazidime
VIM-28variant V258F	Pseudomonas aeruginosa	Cephalothin	260	0.0101	2.58 × 10⁴	pH 7.5, 30 °C	Open record: VIM-28 variant V258F, Cephalothin
VIM-28variant V258F	Pseudomonas aeruginosa	Meropenem	23	0.014	1.64 × 10³	pH 7.5, 30 °C	Open record: VIM-28 variant V258F, Meropenem
VIM-4	Pseudomonas aeruginosa	Ampicillin	197	0.0257	7.67 × 10³	pH 7.5, 30 °C	Open record: VIM-4, Ampicillin
VIM-4	Pseudomonas aeruginosa	benzylpenicillin	812	0.0939	8.65 × 10³	pH 7.5, 30 °C	Open record: VIM-4, benzylpenicillin
VIM-4	Pseudomonas aeruginosa	Cefotaxime	651	0.0418	1.56 × 10⁴	pH 7.5, 30 °C	Open record: VIM-4, Cefotaxime
VIM-4	Pseudomonas aeruginosa	Ceftazidime	—	—	44.1	pH 7.5, 30 °C	Open record: VIM-4, Ceftazidime
VIM-4	Pseudomonas aeruginosa	Cephalothin	756	0.0188	3.99 × 10⁴	pH 7.5, 30 °C	Open record: VIM-4, Cephalothin
VIM-4	Pseudomonas aeruginosa	Meropenem	17	0.00835	2.04 × 10³	pH 7.5, 30 °C	Open record: VIM-4, Meropenem