Values CatLog read from this paper
Role of Aspartate 86 in the Catalytic Mechanism of Escherichia coli Glutamate Decarboxylase
PMID 41717872doi:10.1021/acs.biochem.5c00666Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (60 values) from this paper: glutamate decarboxylase GadB (EcGadB) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glutamate decarboxylase GadB (EcGadB) | Escherichia coli | L-glutamate | 14.21 | 1.04 | 13.66 | pH 4.6, 25 °C | Open record: glutamate decarboxylase GadB (EcGadB), L-glutamate |
| glutamate decarboxylase GadB (EcGadB) | Escherichia coli | L-glutamate4.60 (pL; pD = pH-meter reading + 0.4) | 2.05 | 0.258 | 7.93 | 25 °C | Open record: glutamate decarboxylase GadB (EcGadB), L-glutamate |
| glutamate decarboxylase GadB (EcGadB) | Escherichia coli | L-glutamate4.80 (pL; pD = pH-meter reading + 0.4) | 1.99 | 0.241 | 8.26 | 25 °C | Open record: glutamate decarboxylase GadB (EcGadB), L-glutamate |
| glutamate decarboxylase GadB (EcGadB) | Escherichia coli | L-glutamate5.00 (pL; pD = pH-meter reading + 0.4) | 1.86 | 0.265 | 6.97 | 25 °C | Open record: glutamate decarboxylase GadB (EcGadB), L-glutamate |
| glutamate decarboxylase GadB (EcGadB) | Escherichia coli | L-glutamate | 17.03 | 1.234 | 13.8 | pH 4.8, 25 °C | Open record: glutamate decarboxylase GadB (EcGadB), L-glutamate |
| glutamate decarboxylase GadB (EcGadB) | Escherichia coli | L-glutamate | 14.25 | 0.866 | 16.46 | pH 5, 25 °C | Open record: glutamate decarboxylase GadB (EcGadB), L-glutamate |
| glutamate decarboxylase GadB (EcGadB)variant D86N/H465A | Escherichia coli | L-glutamate | 29.06 | 3.655 | 7.95 | pH 4.6, 25 °C | Open record: glutamate decarboxylase GadB (EcGadB) variant D86N/H465A, L-glutamate |
| glutamate decarboxylase GadB (EcGadB)variant D86N/H465A | Escherichia coli | L-glutamate4.60 (pL; pD = pH-meter reading + 0.4) | 10.96 | 1.435 | 7.64 | 25 °C | Open record: glutamate decarboxylase GadB (EcGadB) variant D86N/H465A, L-glutamate |
| glutamate decarboxylase GadB (EcGadB)variant D86N/H465A | Escherichia coli | L-glutamate4.80 (pL; pD = pH-meter reading + 0.4) | 10.23 | 3.011 | 3.4 | 25 °C | Open record: glutamate decarboxylase GadB (EcGadB) variant D86N/H465A, L-glutamate |
| glutamate decarboxylase GadB (EcGadB)variant D86N/H465A | Escherichia coli | L-glutamate5.00 (pL; pD = pH-meter reading + 0.4) | 7.39 | 1.081 | 6.84 | 25 °C | Open record: glutamate decarboxylase GadB (EcGadB) variant D86N/H465A, L-glutamate |
| glutamate decarboxylase GadB (EcGadB)variant D86N/H465A | Escherichia coli | L-glutamate | 29.81 | 4.143 | 7.2 | pH 4.8, 25 °C | Open record: glutamate decarboxylase GadB (EcGadB) variant D86N/H465A, L-glutamate |
| glutamate decarboxylase GadB (EcGadB)variant D86N/H465A | Escherichia coli | L-glutamate | 5.86 | 5.56 | 1.05 | pH 6.2, 25 °C | Open record: glutamate decarboxylase GadB (EcGadB) variant D86N/H465A, L-glutamate |
| glutamate decarboxylase GadB (EcGadB)variant D86N/H465A | Escherichia coli | L-glutamate | 27.66 | 3.325 | 8.32 | pH 5, 25 °C | Open record: glutamate decarboxylase GadB (EcGadB) variant D86N/H465A, L-glutamate |
| glutamate decarboxylase GadB (EcGadB)variant H465A | Escherichia coli | L-glutamate | 17.02 | 0.819 | 20.78 | pH 4.6, 25 °C | Open record: glutamate decarboxylase GadB (EcGadB) variant H465A, L-glutamate |
| glutamate decarboxylase GadB (EcGadB)variant H465A | Escherichia coli | L-glutamate4.60 (pL; pD = pH-meter reading + 0.4) | 2.51 | 0.281 | 8.93 | 25 °C | Open record: glutamate decarboxylase GadB (EcGadB) variant H465A, L-glutamate |
| glutamate decarboxylase GadB (EcGadB)variant H465A | Escherichia coli | L-glutamate4.80 (pL; pD = pH-meter reading + 0.4) | 2.33 | 0.24 | 9.71 | 25 °C | Open record: glutamate decarboxylase GadB (EcGadB) variant H465A, L-glutamate |
| glutamate decarboxylase GadB (EcGadB)variant H465A | Escherichia coli | L-glutamate5.00 (pL; pD = pH-meter reading + 0.4) | 2.58 | 0.175 | 14.74 | 25 °C | Open record: glutamate decarboxylase GadB (EcGadB) variant H465A, L-glutamate |
| glutamate decarboxylase GadB (EcGadB)variant H465A | Escherichia coli | L-glutamate | 15.57 | 0.767 | 20.3 | pH 4.8, 25 °C | Open record: glutamate decarboxylase GadB (EcGadB) variant H465A, L-glutamate |
| glutamate decarboxylase GadB (EcGadB)variant H465A | Escherichia coli | L-glutamate | 5.34 | 6.18 | 1.16 | pH 6.2, 25 °C | Open record: glutamate decarboxylase GadB (EcGadB) variant H465A, L-glutamate |
| glutamate decarboxylase GadB (EcGadB)variant H465A | Escherichia coli | L-glutamate | 17.83 | 0.982 | 18.16 | pH 5, 25 °C | Open record: glutamate decarboxylase GadB (EcGadB) variant H465A, L-glutamate |