CatLogenzyme kinetics catalog

Values CatLog read from this paper

Decoding the substrate specificity landscape of a promiscuous enzyme through multi-substrate mutational scanning

PMID 41748636doi:10.1038/s41467-026-69913-zOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 223 records (249 values) from this paper: D-amino acid oxidase (DAOx) from Rhodotorula gracilis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 26 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-alanineH2O2 detected by fluorescence at 590 nm; Table 11.475.620.262 calc.pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-alanineH2O2 followed by fluorescence at 590 nm—6.965—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-alanineH2O2 followed by fluorescence at 590 nm—7.316—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-alanineH2O2 followed by fluorescence at 590 nm—2.31—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-alanineH2O2 followed by fluorescence at 590 nm—2.4—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-asparagineH2O2 detected by fluorescence at 590 nm; Table 10.8447.060.018 calc.pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-asparagineH2O2 followed by fluorescence at 590 nm—54.05—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-asparagineH2O2 followed by fluorescence at 590 nm—45.82—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-asparagineH2O2 followed by fluorescence at 590 nm—9.66—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-asparagineH2O2 followed by fluorescence at 590 nm—19.6—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-glutamineH2O2 detected by fluorescence at 590 nm; Table 10.8118.840.043 calc.pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-glutamineH2O2 followed by fluorescence at 590 nm—16.12—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-glutamineH2O2 followed by fluorescence at 590 nm—14.42—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-glutamineH2O2 followed by fluorescence at 590 nm—7.94—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-glutamineH2O2 followed by fluorescence at 590 nm—16.2—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-histidine—3.612—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-histidineAga2 fusion displayed on the surface of S. cerevisiae EBY100—3.406—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-leucine—5.021—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-leucineAga2 fusion displayed on the surface of S. cerevisiae EBY100—4.991—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-methionineH2O2 detected by fluorescence at 590 nm; Table 11.372.020.678 calc.pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-methionineH2O2 followed by fluorescence at 590 nm—2.214—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-methionineH2O2 followed by fluorescence at 590 nm—2.039—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-methionineH2O2 followed by fluorescence at 590 nm—0.49—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-methionineH2O2 followed by fluorescence at 590 nm—0.65—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-phenylalanineH2O2 detected by fluorescence at 590 nm; Table 11.932.560.754 calc.pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-phenylalanineH2O2 followed by fluorescence at 590 nm—2.497—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-phenylalanineH2O2 followed by fluorescence at 590 nm—1.507—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-phenylalanineH2O2 followed by fluorescence at 590 nm—0.45—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-phenylalanineH2O2 followed by fluorescence at 590 nm—0.79—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-serine—27.58—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-serineAga2 fusion displayed on the surface of S. cerevisiae EBY100—16.47—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-threonine—34.01—pH 7.5Open
D-amino acid oxidase (DAOx)Rhodotorula gracilisD-threonineAga2 fusion displayed on the surface of S. cerevisiae EBY100—39.38—pH 7.5Open
D-amino acid oxidase (DAOx)variant A222MRhodotorula gracilisD-alanine—4.44—pH 7.5Open
D-amino acid oxidase (DAOx)variant A222MRhodotorula gracilisD-asparagine—21.11—pH 7.5Open
D-amino acid oxidase (DAOx)variant A222MRhodotorula gracilisD-glutamine—26.91—pH 7.5Open
D-amino acid oxidase (DAOx)variant A222MRhodotorula gracilisD-methionine—2.43—pH 7.5Open
D-amino acid oxidase (DAOx)variant A222MRhodotorula gracilisD-phenylalanine—1.5—pH 7.5Open
D-amino acid oxidase (DAOx)variant E276FRhodotorula gracilisD-alanine—4.48—pH 7.5Open
D-amino acid oxidase (DAOx)variant E276FRhodotorula gracilisD-asparagine—18.14—pH 7.5Open
D-amino acid oxidase (DAOx)variant E276FRhodotorula gracilisD-glutamine—13.9—pH 7.5Open
D-amino acid oxidase (DAOx)variant E276FRhodotorula gracilisD-methionine—0.91—pH 7.5Open
D-amino acid oxidase (DAOx)variant E276FRhodotorula gracilisD-phenylalanine—0.77—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58WRhodotorula gracilisD-alanineH2O2 detected by fluorescence at 590 nm2.2110.430.212 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant F58WRhodotorula gracilisD-alanineH2O2 followed by fluorescence at 590 nm—7.26—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58WRhodotorula gracilisD-asparagineH2O2 detected by fluorescence at 590 nm0.0647.90.0013 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant F58WRhodotorula gracilisD-asparagineH2O2 followed by fluorescence at 590 nm—47.88—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58WRhodotorula gracilisD-glutamineH2O2 detected by fluorescence at 590 nm0.0730.70.0023 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant F58WRhodotorula gracilisD-glutamineH2O2 followed by fluorescence at 590 nm—20.56—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58WRhodotorula gracilisD-methionineH2O2 detected by fluorescence at 590 nm0.438.90.048 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant F58WRhodotorula gracilisD-methionineH2O2 followed by fluorescence at 590 nm—5.5—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58WRhodotorula gracilisD-phenylalanineH2O2 detected by fluorescence at 590 nm0.2243.730.0050 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant F58WRhodotorula gracilisD-phenylalanineH2O2 followed by fluorescence at 590 nm—65.79—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58W/A222MRhodotorula gracilisD-alanine—12.09—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58W/A222MRhodotorula gracilisD-glutamine—16.82—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58W/A222MRhodotorula gracilisD-methionine—12.7—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58W/M213ERhodotorula gracilisD-asparagine—29.92—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58W/M213ERhodotorula gracilisD-glutamine—17.36—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58W/M213ERhodotorula gracilisD-methionine—11.81—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58W/S215YRhodotorula gracilisD-alanine—5.48—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58W/S215YRhodotorula gracilisD-asparagine—70.19—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58W/S215YRhodotorula gracilisD-glutamine—20.02—pH 7.5Open
D-amino acid oxidase (DAOx)variant F58W/S215YRhodotorula gracilisD-methionine—6.99—pH 7.5Open
D-amino acid oxidase (DAOx)variant H258RRhodotorula gracilisD-alanine—5.96—pH 7.5Open
D-amino acid oxidase (DAOx)variant H258RRhodotorula gracilisD-asparagine—41.29—pH 7.5Open
D-amino acid oxidase (DAOx)variant H258RRhodotorula gracilisD-glutamine—23.4—pH 7.5Open
D-amino acid oxidase (DAOx)variant H258RRhodotorula gracilisD-methionine—1.89—pH 7.5Open
D-amino acid oxidase (DAOx)variant H258RRhodotorula gracilisD-phenylalanine—0.63—pH 7.5Open
D-amino acid oxidase (DAOx)variant H258WRhodotorula gracilisD-alanine—6.51—pH 7.5Open
D-amino acid oxidase (DAOx)variant H258WRhodotorula gracilisD-asparagine—11.15—pH 7.5Open
D-amino acid oxidase (DAOx)variant H258WRhodotorula gracilisD-glutamine—18.08—pH 7.5Open
D-amino acid oxidase (DAOx)variant H258WRhodotorula gracilisD-methionine—1.34—pH 7.5Open
D-amino acid oxidase (DAOx)variant H258WRhodotorula gracilisD-phenylalanine—0.55—pH 7.5Open
D-amino acid oxidase (DAOx)variant I197SRhodotorula gracilisD-alanine—1.96—pH 7.5Open
D-amino acid oxidase (DAOx)variant I197SRhodotorula gracilisD-asparagine—14.71—pH 7.5Open
D-amino acid oxidase (DAOx)variant I197SRhodotorula gracilisD-glutamine—10.97—pH 7.5Open
D-amino acid oxidase (DAOx)variant I197SRhodotorula gracilisD-methionine—0.69—pH 7.5Open
D-amino acid oxidase (DAOx)variant I197SRhodotorula gracilisD-phenylalanine—2.16—pH 7.5Open
D-amino acid oxidase (DAOx)variant L262ARhodotorula gracilisD-alanine—5.66—pH 7.5Open
D-amino acid oxidase (DAOx)variant L262ARhodotorula gracilisD-asparagine—57.29—pH 7.5Open
D-amino acid oxidase (DAOx)variant L262ARhodotorula gracilisD-glutamine—26.56—pH 7.5Open
D-amino acid oxidase (DAOx)variant L262ARhodotorula gracilisD-methionine—2.17—pH 7.5Open
D-amino acid oxidase (DAOx)variant L262ARhodotorula gracilisD-phenylalanine—2.79—pH 7.5Open
D-amino acid oxidase (DAOx)variant L262VRhodotorula gracilisD-alanine—2.86—pH 7.5Open
D-amino acid oxidase (DAOx)variant L262VRhodotorula gracilisD-asparagine—34.08—pH 7.5Open
D-amino acid oxidase (DAOx)variant L262VRhodotorula gracilisD-glutamine—33.16—pH 7.5Open
D-amino acid oxidase (DAOx)variant L262VRhodotorula gracilisD-methionine—1.74—pH 7.5Open
D-amino acid oxidase (DAOx)variant L262VRhodotorula gracilisD-phenylalanine—2.52—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213DRhodotorula gracilisD-alanine—55.9—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213DRhodotorula gracilisD-asparagine—8.36—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213DRhodotorula gracilisD-glutamine—5.02—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213DRhodotorula gracilisD-methionine—4.37—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213DRhodotorula gracilisD-phenylalanine—28.24—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213ERhodotorula gracilisD-alanine0.1329.710.0044 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant M213ERhodotorula gracilisD-asparagineH2O2 detected by fluorescence at 590 nm1.317.260.075 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant M213ERhodotorula gracilisD-asparagineH2O2 followed by fluorescence at 590 nm—8.1—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213ERhodotorula gracilisD-glutamineH2O2 detected by fluorescence at 590 nm0.2213.090.017 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant M213ERhodotorula gracilisD-glutamineH2O2 followed by fluorescence at 590 nm—12.37—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213ERhodotorula gracilisD-methionineH2O2 detected by fluorescence at 590 nm1.759.990.175 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant M213ERhodotorula gracilisD-methionineH2O2 followed by fluorescence at 590 nm—9.72—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213ERhodotorula gracilisD-phenylalanineH2O2 detected by fluorescence at 590 nm1.8521.520.0860 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant M213ERhodotorula gracilisD-phenylalanineH2O2 followed by fluorescence at 590 nm—10.34—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213E/S215YRhodotorula gracilisD-alanine—30.37—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213E/S215YRhodotorula gracilisD-asparagine—4.31—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213E/S215YRhodotorula gracilisD-glutamine—14.89—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213E/S215YRhodotorula gracilisD-methionine—10.6—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213E/S215YRhodotorula gracilisD-phenylalanine—26.84—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213HRhodotorula gracilisD-alanine—10.89—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213HRhodotorula gracilisD-asparagine—11.38—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213HRhodotorula gracilisD-glutamine—11.69—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213HRhodotorula gracilisD-methionine—4.93—pH 7.5Open
D-amino acid oxidase (DAOx)variant M213HRhodotorula gracilisD-phenylalanine—5.78—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54IRhodotorula gracilisD-alanine—16.05—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54IRhodotorula gracilisD-asparagine—21.05—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54IRhodotorula gracilisD-glutamine—10.6—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54IRhodotorula gracilisD-methionine—0.49—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54IRhodotorula gracilisD-phenylalanine—0.85—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54LRhodotorula gracilisD-alanineH2O2 detected by fluorescence at 590 nm1.222.040.054 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant N54LRhodotorula gracilisD-alanineH2O2 followed by fluorescence at 590 nm—22.24—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54LRhodotorula gracilisD-asparagineH2O2 detected by fluorescence at 590 nm0.3259.290.0054 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant N54LRhodotorula gracilisD-asparagineH2O2 followed by fluorescence at 590 nm—26.04—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54LRhodotorula gracilisD-glutamineH2O2 detected by fluorescence at 590 nm0.2136.750.0057 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant N54LRhodotorula gracilisD-glutamineH2O2 followed by fluorescence at 590 nm—25.11—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54LRhodotorula gracilisD-methionineH2O2 detected by fluorescence at 590 nm0.91.350.67 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant N54LRhodotorula gracilisD-methionineH2O2 followed by fluorescence at 590 nm—0.91—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54LRhodotorula gracilisD-phenylalanineH2O2 detected by fluorescence at 590 nm1.071.60.67 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant N54LRhodotorula gracilisD-phenylalanineH2O2 followed by fluorescence at 590 nm—0.83—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54L/H258WRhodotorula gracilisD-alanine—23.46—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54L/H258WRhodotorula gracilisD-asparagine—56.58—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54L/H258WRhodotorula gracilisD-glutamine—24.05—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54L/H258WRhodotorula gracilisD-methionine—1.37—pH 7.5Open
D-amino acid oxidase (DAOx)variant N54L/H258WRhodotorula gracilisD-phenylalanine—0.83—pH 7.5Open
D-amino acid oxidase (DAOx)variant Q192CRhodotorula gracilisD-alanine—3.55—pH 7.5Open
D-amino acid oxidase (DAOx)variant Q192CRhodotorula gracilisD-asparagine—24.61—pH 7.5Open
D-amino acid oxidase (DAOx)variant Q192CRhodotorula gracilisD-glutamine—16.85—pH 7.5Open
D-amino acid oxidase (DAOx)variant Q192CRhodotorula gracilisD-methionine—1.02—pH 7.5Open
D-amino acid oxidase (DAOx)variant Q192CRhodotorula gracilisD-phenylalanine—0.87—pH 7.5Open
D-amino acid oxidase (DAOx)variant Q192PRhodotorula gracilisD-alanine—2.56—pH 7.5Open
D-amino acid oxidase (DAOx)variant Q192PRhodotorula gracilisD-asparagine—22.04—pH 7.5Open
D-amino acid oxidase (DAOx)variant Q192PRhodotorula gracilisD-glutamine—17.02—pH 7.5Open
D-amino acid oxidase (DAOx)variant Q192PRhodotorula gracilisD-methionine—0.79—pH 7.5Open
D-amino acid oxidase (DAOx)variant Q192PRhodotorula gracilisD-phenylalanine—0.7—pH 7.5Open
D-amino acid oxidase (DAOx)variant R254CRhodotorula gracilisD-alanine—3.3—pH 7.5Open
D-amino acid oxidase (DAOx)variant R254CRhodotorula gracilisD-asparagine—23.3—pH 7.5Open
D-amino acid oxidase (DAOx)variant R254CRhodotorula gracilisD-glutamine—21.38—pH 7.5Open
D-amino acid oxidase (DAOx)variant R254CRhodotorula gracilisD-methionine—1.52—pH 7.5Open
D-amino acid oxidase (DAOx)variant R254CRhodotorula gracilisD-phenylalanine—0.47—pH 7.5Open
D-amino acid oxidase (DAOx)variant R254DRhodotorula gracilisD-alanine—5.62—pH 7.5Open
D-amino acid oxidase (DAOx)variant R254DRhodotorula gracilisD-asparagine—35.04—pH 7.5Open
D-amino acid oxidase (DAOx)variant R254DRhodotorula gracilisD-glutamine—19.99—pH 7.5Open
D-amino acid oxidase (DAOx)variant R254DRhodotorula gracilisD-methionine—1.67—pH 7.5Open
D-amino acid oxidase (DAOx)variant R254DRhodotorula gracilisD-phenylalanine—1.02—pH 7.5Open
D-amino acid oxidase (DAOx)variant S215YRhodotorula gracilisD-alanine—2.08—pH 7.5Open
D-amino acid oxidase (DAOx)variant S215YRhodotorula gracilisD-asparagine—6.39—pH 7.5Open
D-amino acid oxidase (DAOx)variant S215YRhodotorula gracilisD-glutamine—8.53—pH 7.5Open
D-amino acid oxidase (DAOx)variant S215YRhodotorula gracilisD-methionine—2.07—pH 7.5Open
D-amino acid oxidase (DAOx)variant S215YRhodotorula gracilisD-phenylalanine—14.84—pH 7.5Open
D-amino acid oxidase (DAOx)variant S216GRhodotorula gracilisD-alanine—3.62—pH 7.5Open
D-amino acid oxidase (DAOx)variant S216GRhodotorula gracilisD-asparagine—20.14—pH 7.5Open
D-amino acid oxidase (DAOx)variant S216GRhodotorula gracilisD-glutamine—13.25—pH 7.5Open
D-amino acid oxidase (DAOx)variant S216GRhodotorula gracilisD-methionine—0.6—pH 7.5Open
D-amino acid oxidase (DAOx)variant S216GRhodotorula gracilisD-phenylalanine—0.37—pH 7.5Open
D-amino acid oxidase (DAOx)variant S216HRhodotorula gracilisD-alanine—2.08—pH 7.5Open
D-amino acid oxidase (DAOx)variant S216HRhodotorula gracilisD-asparagine—21.06—pH 7.5Open
D-amino acid oxidase (DAOx)variant S216HRhodotorula gracilisD-glutamine—18.52—pH 7.5Open
D-amino acid oxidase (DAOx)variant S216HRhodotorula gracilisD-methionine—1—pH 7.5Open
D-amino acid oxidase (DAOx)variant S216HRhodotorula gracilisD-phenylalanine—1.08—pH 7.5Open
D-amino acid oxidase (DAOx)variant S220HRhodotorula gracilisD-alanine—3—pH 7.5Open
D-amino acid oxidase (DAOx)variant S220HRhodotorula gracilisD-asparagine—21.22—pH 7.5Open
D-amino acid oxidase (DAOx)variant S220HRhodotorula gracilisD-glutamine—18.18—pH 7.5Open
D-amino acid oxidase (DAOx)variant S220HRhodotorula gracilisD-methionine—0.65—pH 7.5Open
D-amino acid oxidase (DAOx)variant S220HRhodotorula gracilisD-phenylalanine—0.31—pH 7.5Open
D-amino acid oxidase (DAOx)variant S220VRhodotorula gracilisD-alanine—2.82—pH 7.5Open
D-amino acid oxidase (DAOx)variant S220VRhodotorula gracilisD-asparagine—19.35—pH 7.5Open
D-amino acid oxidase (DAOx)variant S220VRhodotorula gracilisD-glutamine—18.34—pH 7.5Open
D-amino acid oxidase (DAOx)variant S220VRhodotorula gracilisD-methionine—0.84—pH 7.5Open
D-amino acid oxidase (DAOx)variant S220VRhodotorula gracilisD-phenylalanine—0.45—pH 7.5Open
D-amino acid oxidase (DAOx)variant S335KRhodotorula gracilisD-asparagine—37.57—pH 7.5Open
D-amino acid oxidase (DAOx)variant S335KRhodotorula gracilisD-glutamine—10.41—pH 7.5Open
D-amino acid oxidase (DAOx)variant S335KRhodotorula gracilisD-methionine—1.47—pH 7.5Open
D-amino acid oxidase (DAOx)variant S335KRhodotorula gracilisD-phenylalanine—6.93—pH 7.5Open
D-amino acid oxidase (DAOx)variant S341IRhodotorula gracilisD-glutamineH2O2 detected by fluorescence at 590 nm0.029.530.0021 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant S341IRhodotorula gracilisD-glutamineH2O2 followed by fluorescence at 590 nm—11.17—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56HRhodotorula gracilisD-alanine—7.15—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56HRhodotorula gracilisD-asparagine—22.77—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56HRhodotorula gracilisD-glutamine—23.93—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56HRhodotorula gracilisD-methionine—3.59—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56HRhodotorula gracilisD-phenylalanine—16.03—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56H/T93MRhodotorula gracilisD-alanine—4.78—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56H/T93MRhodotorula gracilisD-asparagine—77.6—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56H/T93MRhodotorula gracilisD-glutamine—30.07—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56H/T93MRhodotorula gracilisD-methionine—6.64—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56H/T93MRhodotorula gracilisD-phenylalanine—20.67—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56MRhodotorula gracilisD-alanine—12.68—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56MRhodotorula gracilisD-asparagine—31.44—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56MRhodotorula gracilisD-glutamine—7.81—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56MRhodotorula gracilisD-methionine—0.53—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56MRhodotorula gracilisD-phenylalanine—15.18—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56QRhodotorula gracilisD-alanine—11.83—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56QRhodotorula gracilisD-asparagine—10.27—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56QRhodotorula gracilisD-glutamine—8.29—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56QRhodotorula gracilisD-methionine—1.83—pH 7.5Open
D-amino acid oxidase (DAOx)variant T56QRhodotorula gracilisD-phenylalanine—7.1—pH 7.5Open
D-amino acid oxidase (DAOx)variant T93MRhodotorula gracilisD-alanineH2O2 detected by fluorescence at 590 nm0.1516.940.0089 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant T93MRhodotorula gracilisD-alanineH2O2 followed by fluorescence at 590 nm—10.8—pH 7.5Open
D-amino acid oxidase (DAOx)variant T93MRhodotorula gracilisD-asparagineH2O2 detected by fluorescence at 590 nm0.0341.027.3 × 10⁻⁴ calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant T93MRhodotorula gracilisD-asparagineH2O2 followed by fluorescence at 590 nm—32.21—pH 7.5Open
D-amino acid oxidase (DAOx)variant T93MRhodotorula gracilisD-glutamineH2O2 detected by fluorescence at 590 nm0.0620.860.0029 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant T93MRhodotorula gracilisD-glutamineH2O2 followed by fluorescence at 590 nm—19.62—pH 7.5Open
D-amino acid oxidase (DAOx)variant T93MRhodotorula gracilisD-methionineH2O2 detected by fluorescence at 590 nm0.120.740.16 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant T93MRhodotorula gracilisD-methionineH2O2 followed by fluorescence at 590 nm—0.37—pH 7.5Open
D-amino acid oxidase (DAOx)variant T93MRhodotorula gracilisD-phenylalanineH2O2 detected by fluorescence at 590 nm0.0813.750.0058 calc.pH 7.5Open
D-amino acid oxidase (DAOx)variant T93MRhodotorula gracilisD-phenylalanineH2O2 followed by fluorescence at 590 nm—17.01—pH 7.5Open
D-amino acid oxidase (DAOx)variant Y145ARhodotorula gracilisD-alanine—3—pH 7.5Open
D-amino acid oxidase (DAOx)variant Y145ARhodotorula gracilisD-asparagine—23.4—pH 7.5Open
D-amino acid oxidase (DAOx)variant Y145ARhodotorula gracilisD-glutamine—17.27—pH 7.5Open
D-amino acid oxidase (DAOx)variant Y145ARhodotorula gracilisD-methionine—0.94—pH 7.5Open
D-amino acid oxidase (DAOx)variant Y145ARhodotorula gracilisD-phenylalanine—0.79—pH 7.5Open
D-amino acid oxidase (DAOx)variant Y145WRhodotorula gracilisD-alanine—3.49—pH 7.5Open
D-amino acid oxidase (DAOx)variant Y145WRhodotorula gracilisD-asparagine—25.01—pH 7.5Open
D-amino acid oxidase (DAOx)variant Y145WRhodotorula gracilisD-glutamine—18.42—pH 7.5Open
D-amino acid oxidase (DAOx)variant Y145WRhodotorula gracilisD-methionine—0.98—pH 7.5Open
D-amino acid oxidase (DAOx)variant Y145WRhodotorula gracilisD-phenylalanine—0.78—pH 7.5Open