Values CatLog read from this paper
Using a bioluminescence resonance energy transfer caspase biosensor to study caspase-3 cleavage site specificity
PMID 41816988doi:10.1042/bsr20254030Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 45 records (45 values) from this paper: caspase-3, caspase-7.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| caspase-3 | — | AEVD biosensor (GFP10-AEVD|G-RLucII BRET2 protein substrate) | — | — | 13 | pH 7.2, 37 °C | Open record: caspase-3, AEVD biosensor (GFP10-AEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | CEVD biosensor (GFP10-CEVD|G-RLucII BRET2 protein substrate) | — | — | 34 | pH 7.2, 37 °C | Open record: caspase-3, CEVD biosensor (GFP10-CEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | DEVD biosensor (GFP10-DEVD|G-RLucII BRET2 protein substrate) | — | — | 59 | pH 7.2, 37 °C | Open record: caspase-3, DEVD biosensor (GFP10-DEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | DEVD biosensor (GFP10-DEVD|G-RLucII BRET2 protein substrate)BRET2 cleavage assay: purified DEVD biosensor (10 nM) | — | — | 60.3 | pH 7.2, 37 °C | Open record: caspase-3, DEVD biosensor (GFP10-DEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | DEVD biosensor (GFP10-DEVD|G-RLucII BRET2 protein substrate)25 nM caspase-3 | — | — | 53.95 | pH 7.2, 37 °C | Open record: caspase-3, DEVD biosensor (GFP10-DEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | EEVD biosensor (GFP10-EEVD|G-RLucII BRET2 protein substrate) | — | — | 17 | pH 7.2, 37 °C | Open record: caspase-3, EEVD biosensor (GFP10-EEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | FEVD biosensor (GFP10-FEVD|G-RLucII BRET2 protein substrate) | — | — | 6.4 | pH 7.2, 37 °C | Open record: caspase-3, FEVD biosensor (GFP10-FEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | GEVD biosensor (GFP10-GEVD|G-RLucII BRET2 protein substrate) | — | — | 2.2 | pH 7.2, 37 °C | Open record: caspase-3, GEVD biosensor (GFP10-GEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a AEVD↓G caspase cleavage site in the linker (P4 = A) | — | — | 13 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a AEVD↓G caspase cleavage site in the linker (P4 = A) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a CEVD↓G caspase cleavage site in the linker (P4 = C) | — | — | 34 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a CEVD↓G caspase cleavage site in the linker (P4 = C) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a DEVD↓G caspase cleavage site in the linker (P4 = D) | — | — | 59 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a DEVD↓G caspase cleavage site in the linker (P4 = D) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a EEVD↓G caspase cleavage site in the linker (P4 = E) | — | — | 17 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a EEVD↓G caspase cleavage site in the linker (P4 = E) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a FEVD↓G caspase cleavage site in the linker (P4 = F) | — | — | 6.4 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a FEVD↓G caspase cleavage site in the linker (P4 = F) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a GEVD↓G caspase cleavage site in the linker (P4 = G) | — | — | 2.2 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a GEVD↓G caspase cleavage site in the linker (P4 = G) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a HEVD↓G caspase cleavage site in the linker (P4 = H) | — | — | 18 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a HEVD↓G caspase cleavage site in the linker (P4 = H) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a IEVD↓G caspase cleavage site in the linker (P4 = I) | — | — | 11 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a IEVD↓G caspase cleavage site in the linker (P4 = I) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a LEVD↓G caspase cleavage site in the linker (P4 = L) | — | — | 6 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a LEVD↓G caspase cleavage site in the linker (P4 = L) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a MEVD↓G caspase cleavage site in the linker (P4 = M) | — | — | 2.3 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a MEVD↓G caspase cleavage site in the linker (P4 = M) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a NEVD↓G caspase cleavage site in the linker (P4 = N) | — | — | 16 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a NEVD↓G caspase cleavage site in the linker (P4 = N) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a PEVD↓G caspase cleavage site in the linker (P4 = P) | — | — | 4.2 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a PEVD↓G caspase cleavage site in the linker (P4 = P) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a QEVD↓G caspase cleavage site in the linker (P4 = Q) | — | — | 7.8 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a QEVD↓G caspase cleavage site in the linker (P4 = Q) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a SEVD↓G caspase cleavage site in the linker (P4 = S) | — | — | 26 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a SEVD↓G caspase cleavage site in the linker (P4 = S) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a TEVD↓G caspase cleavage site in the linker (P4 = T) | — | — | 28 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a TEVD↓G caspase cleavage site in the linker (P4 = T) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a VEVD↓G caspase cleavage site in the linker (P4 = V) | — | — | 17 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a VEVD↓G caspase cleavage site in the linker (P4 = V) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a WEVD↓G caspase cleavage site in the linker (P4 = W) | — | — | 1.7 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a WEVD↓G caspase cleavage site in the linker (P4 = W) |
| caspase-3 | — | GFP10-RLucII BRET^2 biosensor fusion protein with a YEVD↓G caspase cleavage site in the linker (P4 = Y) | — | — | 8.8 | pH 7.2, 37 °C | Open record: caspase-3, GFP10-RLucII BRET^2 biosensor fusion protein with a YEVD↓G caspase cleavage site in the linker (P4 = Y) |
| caspase-3 | — | HEVD biosensor (GFP10-HEVD|G-RLucII BRET2 protein substrate) | — | — | 18 | pH 7.2, 37 °C | Open record: caspase-3, HEVD biosensor (GFP10-HEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | IEVD biosensor (GFP10-IEVD|G-RLucII BRET2 protein substrate) | — | — | 11 | pH 7.2, 37 °C | Open record: caspase-3, IEVD biosensor (GFP10-IEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | LEVD biosensor (GFP10-LEVD|G-RLucII BRET2 protein substrate) | — | — | 6 | pH 7.2, 37 °C | Open record: caspase-3, LEVD biosensor (GFP10-LEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | MEVD biosensor (GFP10-MEVD|G-RLucII BRET2 protein substrate) | — | — | 2.3 | pH 7.2, 37 °C | Open record: caspase-3, MEVD biosensor (GFP10-MEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | NEVD biosensor (GFP10-NEVD|G-RLucII BRET2 protein substrate) | — | — | 16 | pH 7.2, 37 °C | Open record: caspase-3, NEVD biosensor (GFP10-NEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | PEVD biosensor (GFP10-PEVD|G-RLucII BRET2 protein substrate) | — | — | 4.2 | pH 7.2, 37 °C | Open record: caspase-3, PEVD biosensor (GFP10-PEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | QEVD biosensor (GFP10-QEVD|G-RLucII BRET2 protein substrate) | — | — | 7.8 | pH 7.2, 37 °C | Open record: caspase-3, QEVD biosensor (GFP10-QEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | SEVD biosensor (GFP10-SEVD|G-RLucII BRET2 protein substrate) | — | — | 26 | pH 7.2, 37 °C | Open record: caspase-3, SEVD biosensor (GFP10-SEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | TEVD biosensor (GFP10-TEVD|G-RLucII BRET2 protein substrate) | — | — | 28 | pH 7.2, 37 °C | Open record: caspase-3, TEVD biosensor (GFP10-TEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | VEVD biosensor (GFP10-VEVD|G-RLucII BRET2 protein substrate) | — | — | 17 | pH 7.2, 37 °C | Open record: caspase-3, VEVD biosensor (GFP10-VEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | WEVD biosensor (GFP10-WEVD|G-RLucII BRET2 protein substrate) | — | — | 1.7 | pH 7.2, 37 °C | Open record: caspase-3, WEVD biosensor (GFP10-WEVD|G-RLucII BRET2 protein substrate) |
| caspase-3 | — | YEVD biosensor (GFP10-YEVD|G-RLucII BRET2 protein substrate) | — | — | 8.8 | pH 7.2, 37 °C | Open record: caspase-3, YEVD biosensor (GFP10-YEVD|G-RLucII BRET2 protein substrate) |
| caspase-7 | — | AEVD biosensor (GFP10-AEVD|G-RLucII BRET2 protein substrate) | — | — | 2.1 | pH 7.2, 37 °C | Open record: caspase-7, AEVD biosensor (GFP10-AEVD|G-RLucII BRET2 protein substrate) |
| caspase-7 | — | CEVD biosensor (GFP10-CEVD|G-RLucII BRET2 protein substrate) | — | — | 1.6 | pH 7.2, 37 °C | Open record: caspase-7, CEVD biosensor (GFP10-CEVD|G-RLucII BRET2 protein substrate) |
| caspase-7 | — | DEVD biosensor (GFP10-DEVD|G-RLucII BRET2 protein substrate) | — | — | 14 | pH 7.2, 37 °C | Open record: caspase-7, DEVD biosensor (GFP10-DEVD|G-RLucII BRET2 protein substrate) |
| caspase-7 | — | EEVD biosensor (GFP10-EEVD|G-RLucII BRET2 protein substrate) | — | — | 0.88 | pH 7.2, 37 °C | Open record: caspase-7, EEVD biosensor (GFP10-EEVD|G-RLucII BRET2 protein substrate) |
| caspase-7 | — | HEVD biosensor (GFP10-HEVD|G-RLucII BRET2 protein substrate) | — | — | 1.6 | pH 7.2, 37 °C | Open record: caspase-7, HEVD biosensor (GFP10-HEVD|G-RLucII BRET2 protein substrate) |
| caspase-7 | — | SEVD biosensor (GFP10-SEVD|G-RLucII BRET2 protein substrate) | — | — | 4.6 | pH 7.2, 37 °C | Open record: caspase-7, SEVD biosensor (GFP10-SEVD|G-RLucII BRET2 protein substrate) |
| caspase-7 | — | TEVD biosensor (GFP10-TEVD|G-RLucII BRET2 protein substrate) | — | — | 2.1 | pH 7.2, 37 °C | Open record: caspase-7, TEVD biosensor (GFP10-TEVD|G-RLucII BRET2 protein substrate) |