Values CatLog read from this paper
The Role of Metal Complexation in the Unfolding Energetics of a Nudix Hydrolase
PMID 41995431doi:10.1021/acs.biochem.6c00030Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (12 values) from this paper: dihydroneopterin triphosphate pyrophosphatase (DHNTPase; Orf17) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dihydroneopterin triphosphate pyrophosphatase (DHNTPase; Orf17) | Escherichia coli | dihydroneopterin triphosphateapo enzyme form (no Co2+/Ni2+ sulfate cluster); 5.0 mM MgCl2 | 0.45 | 0.0056 | 80 calc. | pH 7.5, 37 °C | Open record: dihydroneopterin triphosphate pyrophosphatase (DHNTPase; Orf17), dihydroneopterin triphosphate |
| dihydroneopterin triphosphate pyrophosphatase (DHNTPase; Orf17) | Escherichia coli | dihydroneopterin triphosphateapo enzyme form (no Co2+/Ni2+ sulfate cluster); 5.0 mM MgCl2 | 0.44 | 0.0056 | 79 calc. | pH 7.5, 37 °C | Open record: dihydroneopterin triphosphate pyrophosphatase (DHNTPase; Orf17), dihydroneopterin triphosphate |
| dihydroneopterin triphosphate pyrophosphatase (DHNTPase; Orf17) | Escherichia coli | dihydroneopterin triphosphateCo2+/SO42- -bound enzyme form; 20 mM HEPES pH 7.5 | 0.45 | 0.0055 | 82 calc. | pH 7.5, 37 °C | Open record: dihydroneopterin triphosphate pyrophosphatase (DHNTPase; Orf17), dihydroneopterin triphosphate |
| dihydroneopterin triphosphate pyrophosphatase (DHNTPase; Orf17) | Escherichia coli | dihydroneopterin triphosphateCo2+/SO42- -bound enzyme form; 20 mM HEPES pH 7.5 | 0.45 | 0.0056 | 80 calc. | pH 7.5, 37 °C | Open record: dihydroneopterin triphosphate pyrophosphatase (DHNTPase; Orf17), dihydroneopterin triphosphate |
| dihydroneopterin triphosphate pyrophosphatase (DHNTPase; Orf17) | Escherichia coli | dihydroneopterin triphosphateNi2+/SO42- -bound enzyme form | 0.44 | 0.0057 | 77 calc. | pH 7.5, 37 °C | Open record: dihydroneopterin triphosphate pyrophosphatase (DHNTPase; Orf17), dihydroneopterin triphosphate |
| dihydroneopterin triphosphate pyrophosphatase (DHNTPase; Orf17) | Escherichia coli | dihydroneopterin triphosphateNi2+/SO42- -bound enzyme form | 0.44 | 0.0056 | 79 calc. | pH 7.5, 37 °C | Open record: dihydroneopterin triphosphate pyrophosphatase (DHNTPase; Orf17), dihydroneopterin triphosphate |