Values CatLog read from this paper
Enzyme structure and kinetics produce tRNA and nucleotide specificity of Schizosaccharomyces pombe CC- and A-adding enzymes
PMID 42130072doi:10.1093/nar/gkag475Open in Browse
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CatLog read 6 records (18 values) from this paper: CCA1 (CC-adding enzyme, tRNA nucleotidyltransferase), CCA2 (A-adding enzyme, tRNA nucleotidyltransferase) from Schizosaccharomyces pombe.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CCA1 (CC-adding enzyme, tRNA nucleotidyltransferase) | Schizosaccharomyces pombe | adenosine 5'-triphosphate | 0.024 | 0.75 | 0.03 | pH 8, 25 °C | Open record: CCA1 (CC-adding enzyme, tRNA nucleotidyltransferase), adenosine 5'-triphosphate |
| CCA1 (CC-adding enzyme, tRNA nucleotidyltransferase) | Schizosaccharomyces pombe | cytidine 5'-triphosphate6 nM CCA1; 3 μM tRNAD; CTP 2–500 μM (Fig. 5D) | 0.49 | 0.008 | 60 | pH 8, 25 °C | Open record: CCA1 (CC-adding enzyme, tRNA nucleotidyltransferase), cytidine 5'-triphosphate |
| CCA1 (CC-adding enzyme, tRNA nucleotidyltransferase) | Schizosaccharomyces pombe | cytidine 5'-triphosphate2 nM CCA1; 3 μM tRNAC; CTP 5–500 μM (Fig. 5E) | 3.4 | 0.04 | 90 | pH 8, 25 °C | Open record: CCA1 (CC-adding enzyme, tRNA nucleotidyltransferase), cytidine 5'-triphosphate |
| CCA1 (CC-adding enzyme, tRNA nucleotidyltransferase) | Schizosaccharomyces pombe | cytidine 5'-triphosphate5 or 10 nM CCA1; 3 μM tRNACC | 0.021 | 0.05 | 0.4 | pH 8, 25 °C | Open record: CCA1 (CC-adding enzyme, tRNA nucleotidyltransferase), cytidine 5'-triphosphate |
| CCA2 (A-adding enzyme, tRNA nucleotidyltransferase) | Schizosaccharomyces pombe | adenosine 5'-triphosphate | 21.7 | 0.18 | 120 | pH 8, 25 °C | Open record: CCA2 (A-adding enzyme, tRNA nucleotidyltransferase), adenosine 5'-triphosphate |
| CCA2 (A-adding enzyme, tRNA nucleotidyltransferase) | Schizosaccharomyces pombe | cytidine 5'-triphosphate | 0.23 | 0.58 | 0.4 | pH 8, 25 °C | Open record: CCA2 (A-adding enzyme, tRNA nucleotidyltransferase), cytidine 5'-triphosphate |