Values CatLog read from this paper
Kinetics study reveals interconnected relationships of component activities in trifunctional RNA capping enzymes
PMID 42248456doi:10.1016/j.jbc.2026.113237Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (30 values) from this paper: Faustovirus RNA capping enzyme (FCE), Vaccinia virus RNA capping enzyme (VCE) from Faustovirus, Vaccinia virus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Faustovirus RNA capping enzyme (FCE) | Faustovirus | 5'-diphosphate RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled)isolated guanylyltransferase (GTase) reaction | 1.1 | 3.158 × 10⁻⁴ | 3.6 × 10³ | 25 °C | Open record: Faustovirus RNA capping enzyme (FCE), 5'-diphosphate RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled) |
| Faustovirus RNA capping enzyme (FCE) | Faustovirus | 5'-diphosphate RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled)no SAM | 1.3 | 3.556 × 10⁻⁴ | 3.5 × 10³ | 25 °C | Open record: Faustovirus RNA capping enzyme (FCE), 5'-diphosphate RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled) |
| Faustovirus RNA capping enzyme (FCE) | Faustovirus | 5'-guanylylated (GpppN-capped) RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled)isolated N7-methyltransferase (N7-MTase) reaction; SAM only | 0.38 | 3.47 × 10⁻⁵ | 1.1 × 10⁴ | 25 °C | Open record: Faustovirus RNA capping enzyme (FCE), 5'-guanylylated (GpppN-capped) RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled) |
| Faustovirus RNA capping enzyme (FCE) | Faustovirus | 5'-guanylylated (GpppN-capped) RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled)15 U qCIP and 6 U apyrase; no SAM or GTP | 0.07 | 2.237 × 10⁻⁴ | 310 | 25 °C | Open record: Faustovirus RNA capping enzyme (FCE), 5'-guanylylated (GpppN-capped) RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled) |
| Faustovirus RNA capping enzyme (FCE) | Faustovirus | 5'-triphosphate RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled) | 2.6 | 1.739 × 10⁻⁴ | 1.5 × 10⁴ | 25 °C | Open record: Faustovirus RNA capping enzyme (FCE), 5'-triphosphate RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled) |
| Vaccinia virus RNA capping enzyme (VCE) | Vaccinia virus | 5'-diphosphate RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled)isolated guanylyltransferase (GTase) reaction | 0.09 | 7.03 × 10⁻⁵ | 1.2 × 10³ | 25 °C | Open record: Vaccinia virus RNA capping enzyme (VCE), 5'-diphosphate RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled) |
| Vaccinia virus RNA capping enzyme (VCE) | Vaccinia virus | 5'-diphosphate RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled)no SAM | 0.08 | 7.48 × 10⁻⁵ | 1.4 × 10³ | 25 °C | Open record: Vaccinia virus RNA capping enzyme (VCE), 5'-diphosphate RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled) |
| Vaccinia virus RNA capping enzyme (VCE) | Vaccinia virus | 5'-guanylylated (GpppN-capped) RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled)isolated N7-methyltransferase (N7-MTase) reaction; SAM only | 0.1 | 3.67 × 10⁻⁵ | 2.6 × 10³ | 25 °C | Open record: Vaccinia virus RNA capping enzyme (VCE), 5'-guanylylated (GpppN-capped) RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled) |
| Vaccinia virus RNA capping enzyme (VCE) | Vaccinia virus | 5'-guanylylated (GpppN-capped) RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled)15 U qCIP and 6 U apyrase; no SAM or GTP | 0.09 | 2.818 × 10⁻⁴ | 340 | 25 °C | Open record: Vaccinia virus RNA capping enzyme (VCE), 5'-guanylylated (GpppN-capped) RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled) |
| Vaccinia virus RNA capping enzyme (VCE) | Vaccinia virus | 5'-triphosphate RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled) | 1 | 8.75 × 10⁻⁵ | 1.1 × 10⁴ | 25 °C | Open record: Vaccinia virus RNA capping enzyme (VCE), 5'-triphosphate RNA oligonucleotide (25 nt, GUAGAACUUCGUCGAGUACGCUCAA, 3'-6-FAM labelled) |