CatLogenzyme kinetics catalog

Values CatLog read from this paper

Target RNA-triggered CRISPR-Cas12a2 preferentially cleaves collateral DNA over RNA

PMID 42301915doi:10.1093/nar/gkag578Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 31 records (61 values) from this paper: Cas12a2 from Sulfuricurvum sp. PC08-66.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
Cas12a2Sulfuricurvum sp. PC08-66dsDNA0.09375 μM phosphorothioated PT-ssDNA—9 × 10⁻⁵—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66dsDNA0.1875 μM phosphorothioated PT-ssDNA—2 × 10⁻⁴—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66dsDNA0.75 μM phosphorothioated PT-ssDNA—2.7 × 10⁻⁴—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66dsDNA0.375 μM phosphorothioated PT-ssRNA—1.1 × 10⁻⁴—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66dsDNA0.75 μM phosphorothioated PT-ssRNA—1.7 × 10⁻⁴—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66dsDNA 5′-F-TGTAACGACC-Q-3′ 3′- ACATTGCTGG -5′0.0124.2 × 10⁻⁵290pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66dsDNA(Quencher on opposite strand) 5′-F-TGTAACGACC--3′ 3′- ACATTGCTGG-Q-5′0.01454 × 10⁻⁵360pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66ssDNA0.375 μM phosphorothioated PT-ssRNA—9 × 10⁻⁵—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66ssDNA0.75 μM phosphorothioated PT-ssRNA—1.8 × 10⁻⁴—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66ssDNA1.5 μM phosphorothioated PT-ssRNA—2.1 × 10⁻⁴—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66ssDNA3.0 μM phosphorothioated PT-ssRNA—3.4 × 10⁻⁴—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66ssDNA0.75 μM phosphorothioated PT-ssDNA—2.4 × 10⁻⁴—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66ssDNA1.5 μM phosphorothioated PT-ssDNA—4.6 × 10⁻⁴—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66ssDNA3.0 μM phosphorothioated PT-ssDNA—7.1 × 10⁻⁴—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66ssDNA 5′-F-TGTAACGACC-Q-3′0.01232.5 × 10⁻⁵490pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66ssRNA0.375 μM phosphorothioated—3.3 × 10⁻⁴—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66ssRNA0.75 μM phosphorothioated—5.5 × 10⁻⁴—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66ssRNA1.5 μM phosphorothioated—0.0015—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66ssRNA3.0 μM phosphorothioated—0.0027—pH 7.9Open
Cas12a2Sulfuricurvum sp. PC08-66ssRNA 5′-F-UGUAACGACC-Q-3′0.0269 × 10⁻⁵290pH 7.9Open
Cas12a2variant F1092ASulfuricurvum sp. PC08-66ssDNA 5′-F-TGTAACGACC-Q-3′0.00362.5 × 10⁻⁴14pH 7.9Open
Cas12a2variant F1092ASulfuricurvum sp. PC08-66ssRNA 5′-F-UGUAACGACC-Q-3′0.030.003610pH 7.9Open
Cas12a2variant Y1069ASulfuricurvum sp. PC08-66dsDNA 5′-F-TGTAACGACC-Q-3′ 3′- ACATTGCTGG -5′0.01271 × 10⁻⁴130pH 7.9Open
Cas12a2variant Y1069ASulfuricurvum sp. PC08-66ssDNA 5′-F-TGTAACGACC-Q-3′0.01673 × 10⁻⁵560pH 7.9Open
Cas12a2variant Y1069ASulfuricurvum sp. PC08-66ssRNA 5′-F-UGUAACGACC-Q-3′0.03871.6 × 10⁻⁴240pH 7.9Open
Cas12a2variant Y1080ASulfuricurvum sp. PC08-66dsDNA 5′-F-TGTAACGACC-Q-3′ 3′- ACATTGCTGG -5′0.00933 × 10⁻⁵310pH 7.9Open
Cas12a2variant Y1080ASulfuricurvum sp. PC08-66ssDNA 5′-F-TGTAACGACC-Q-3′0.01261.5 × 10⁻⁵840pH 7.9Open
Cas12a2variant Y1080ASulfuricurvum sp. PC08-66ssRNA 5′-F-UGUAACGACC-Q-3′0.02391.2 × 10⁻⁴200pH 7.9Open
Cas12a2variant Y465ASulfuricurvum sp. PC08-66dsDNA 5′-F-TGTAACGACC-Q-3′ 3′- ACATTGCTGG -5′0.0121 × 10⁻⁴120pH 7.9Open
Cas12a2variant Y465ASulfuricurvum sp. PC08-66ssDNA 5′-F-TGTAACGACC-Q-3′0.01622.5 × 10⁻⁵650pH 7.9Open
Cas12a2variant Y465ASulfuricurvum sp. PC08-66ssRNA 5′-F-UGUAACGACC-Q-3′0.02811.04 × 10⁻⁴270pH 7.9Open