Values CatLog read from this paper
Basic-patch mutations in bacteriophage T4 Rad50 uncouple ATPase activation from processive nuclease activity
PMID 42417150doi:10.1042/bsr20260367Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (22 values) from this paper: Rad50 from bacteriophage T4.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Rad50 | bacteriophage T4 | adenosine triphosphatealone (R) | 0.06 | 0.031 | 1.9 calc. | pH 7.6, 30 °C | Open record: Rad50, adenosine triphosphate |
| Rad50 | bacteriophage T4 | adenosine triphosphate+ Mre11 (MR complex; Mre11 in 1.25-fold excess over Rad50) | 0.14 | 0.03 | 4.7 calc. | pH 7.6, 30 °C | Open record: Rad50, adenosine triphosphate |
| Rad50 | bacteriophage T4 | adenosine triphosphate | 1.97 | — | — | pH 7.6, 30 °C | Open record: Rad50, adenosine triphosphate |
| Rad50variant D479A | bacteriophage T4 | adenosine triphosphatealone (R) | 0.045 | — | — | pH 7.6, 30 °C | Open record: Rad50 variant D479A, adenosine triphosphate |
| Rad50variant D479A | bacteriophage T4 | adenosine triphosphate+ Mre11 (MR complex; Mre11 in 1.25-fold excess over Rad50) | 0.5 | — | — | pH 7.6, 30 °C | Open record: Rad50 variant D479A, adenosine triphosphate |
| Rad50variant D479A | bacteriophage T4 | adenosine triphosphate | 1.09 | — | — | pH 7.6, 30 °C | Open record: Rad50 variant D479A, adenosine triphosphate |
| Rad50variant K156A | bacteriophage T4 | adenosine triphosphatealone (R) | 0.045 | — | — | pH 7.6, 30 °C | Open record: Rad50 variant K156A, adenosine triphosphate |
| Rad50variant K156A | bacteriophage T4 | adenosine triphosphate+ Mre11 (MR complex; Mre11 in 1.25-fold excess over Rad50) | 0.13 | — | — | pH 7.6, 30 °C | Open record: Rad50 variant K156A, adenosine triphosphate |
| Rad50variant K156A | bacteriophage T4 | adenosine triphosphate | 1.9 | — | — | pH 7.6, 30 °C | Open record: Rad50 variant K156A, adenosine triphosphate |
| Rad50variant R154A | bacteriophage T4 | adenosine triphosphatealone (R) | 0.036 | 0.7 | 0.051 calc. | pH 7.6, 30 °C | Open record: Rad50 variant R154A, adenosine triphosphate |
| Rad50variant R154A | bacteriophage T4 | adenosine triphosphate+ Mre11 (MR complex; Mre11 in 1.25-fold excess over Rad50) | 1.1 | — | — | pH 7.6, 30 °C | Open record: Rad50 variant R154A, adenosine triphosphate |
| Rad50variant R154A | bacteriophage T4 | adenosine triphosphate | 1.24 | — | — | pH 7.6, 30 °C | Open record: Rad50 variant R154A, adenosine triphosphate |
| Rad50variant R154A/R155A/K156A (TripleA) | bacteriophage T4 | adenosine triphosphatealone (R) | 0.012 | 1.6 | 0.0075 calc. | pH 7.6, 30 °C | Open record: Rad50 variant R154A/R155A/K156A (TripleA), adenosine triphosphate |
| Rad50variant R154A/R155A/K156A (TripleA) | bacteriophage T4 | adenosine triphosphate+ Mre11 (MR complex; Mre11 in 1.25-fold excess over Rad50) | 0.84 | — | — | pH 7.6, 30 °C | Open record: Rad50 variant R154A/R155A/K156A (TripleA), adenosine triphosphate |
| Rad50variant R154A/R155A/K156A (TripleA) | bacteriophage T4 | adenosine triphosphate | 0.73 | — | — | pH 7.6, 30 °C | Open record: Rad50 variant R154A/R155A/K156A (TripleA), adenosine triphosphate |
| Rad50variant R155A | bacteriophage T4 | adenosine triphosphatealone (R) | 0.062 | — | — | pH 7.6, 30 °C | Open record: Rad50 variant R155A, adenosine triphosphate |
| Rad50variant R155A | bacteriophage T4 | adenosine triphosphate+ Mre11 (MR complex; Mre11 in 1.25-fold excess over Rad50) | 0.29 | — | — | pH 7.6, 30 °C | Open record: Rad50 variant R155A, adenosine triphosphate |
| Rad50variant R155A | bacteriophage T4 | adenosine triphosphate | 1.51 | — | — | pH 7.6, 30 °C | Open record: Rad50 variant R155A, adenosine triphosphate |