Values CatLog read from this paper
Single-residue engineering of Klenow fragment enables broad acceptance of chemically modified nucleotides
PMID 42454372doi:10.1093/nar/gkag697Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (24 values) from this paper: DNA polymerase I Klenow fragment from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| DNA polymerase I Klenow fragmentvariant D355A/E357A (Kle-WT) | Escherichia coli | 2'-deoxy-2'-fluoroadenosine 5'-triphosphate | 0.01 | 0.02268 | 0.44 | 37 °C | Open record: DNA polymerase I Klenow fragment variant D355A/E357A (Kle-WT), 2'-deoxy-2'-fluoroadenosine 5'-triphosphate |
| DNA polymerase I Klenow fragmentvariant D355A/E357A (Kle-WT) | Escherichia coli | 2'-deoxyadenosine 5'-triphosphate | 0.11 | 4.2 × 10⁻⁴ | 260 | 37 °C | Open record: DNA polymerase I Klenow fragment variant D355A/E357A (Kle-WT), 2'-deoxyadenosine 5'-triphosphate |
| DNA polymerase I Klenow fragmentvariant D355A/E357A (Kle-WT) | Escherichia coli | adenosine 5'-triphosphate | 0.06 | 0.06788 | 0.88 | 37 °C | Open record: DNA polymerase I Klenow fragment variant D355A/E357A (Kle-WT), adenosine 5'-triphosphate |
| DNA polymerase I Klenow fragmentvariant D355A/E357A (Kle-WT) | Escherichia coli | faATP | 0.05 | 0.07323 | 0.66 | 37 °C | Open record: DNA polymerase I Klenow fragment variant D355A/E357A (Kle-WT), faATP |
| DNA polymerase I Klenow fragmentvariant D355A/E357A/F762A (F762A) | Escherichia coli | 2'-deoxy-2'-fluoroadenosine 5'-triphosphate | 0.04 | 0.001 | 40 | 37 °C | Open record: DNA polymerase I Klenow fragment variant D355A/E357A/F762A (F762A), 2'-deoxy-2'-fluoroadenosine 5'-triphosphate |
| DNA polymerase I Klenow fragmentvariant D355A/E357A/F762A (F762A) | Escherichia coli | 2'-deoxyadenosine 5'-triphosphate | 0.11 | 5.2 × 10⁻⁴ | 210 | 37 °C | Open record: DNA polymerase I Klenow fragment variant D355A/E357A/F762A (F762A), 2'-deoxyadenosine 5'-triphosphate |
| DNA polymerase I Klenow fragmentvariant D355A/E357A/F762A (F762A) | Escherichia coli | adenosine 5'-triphosphate | 0.03 | 0.00281 | 11 | 37 °C | Open record: DNA polymerase I Klenow fragment variant D355A/E357A/F762A (F762A), adenosine 5'-triphosphate |
| DNA polymerase I Klenow fragmentvariant D355A/E357A/F762A (F762A) | Escherichia coli | faATP | 0.03 | 0.00237 | 13 | 37 °C | Open record: DNA polymerase I Klenow fragment variant D355A/E357A/F762A (F762A), faATP |