CatLogenzyme kinetics catalog

Values CatLog read from this paper

The β2αB loop determines NAD(P) cofactor specificity and kinetics in trypanosomal D-3-hydroxybutyrate dehydrogenases

PMID 42456964doi:10.1016/j.jmb.2026.169946Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 46 records (138 values) from this paper: D-3-hydroxybutyrate dehydrogenase (TcHBDH), D-3-hydroxybutyrate dehydrogenase (TbHBDH) from Trypanosoma cruzi, Trypanosoma brucei.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.30

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
D-3-hydroxybutyrate dehydrogenase (TbHBDH)Trypanosoma bruceiD-3-hydroxybutyratecosubstrate NADP+140.3143pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TbHBDH)Trypanosoma bruceiD-3-hydroxybutyratecosubstrate NAD+220.6832pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TbHBDH)Trypanosoma bruceiNAD+180.16120pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TbHBDH)Trypanosoma bruceiNADP+9.10.1559.4pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TbHBDH)variant K36RTrypanosoma bruceiD-3-hydroxybutyratecosubstrate NADP+130.3538pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TbHBDH)variant K36RTrypanosoma bruceiD-3-hydroxybutyratecosubstrate NAD+120.2943pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TbHBDH)variant K36RTrypanosoma bruceiNAD+330.3888pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TbHBDH)variant K36RTrypanosoma bruceiNADP+13.50.2455pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)Trypanosoma cruziacetoacetate5.220.011460pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)Trypanosoma cruziD-3-hydroxybutyrate4.10.1137pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)Trypanosoma cruziNADP+3.60.008450pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)Trypanosoma cruziNADPH3.70.018207pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant C64YTrypanosoma cruziD-3-hydroxybutyratecosubstrate NADP+6.90.877.9pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant C64YTrypanosoma cruziD-3-hydroxybutyratecosubstrate NAD+0.970.293.3pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant C64YTrypanosoma cruziNAD+1.70.247pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant C64YTrypanosoma cruziNADP+150.043350pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant K20STrypanosoma cruziD-3-hydroxybutyratecosubstrate NADP+490.4120pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant K20STrypanosoma cruziD-3-hydroxybutyratecosubstrate NAD+430.8849pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant K20STrypanosoma cruziNAD+462.519pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant K20STrypanosoma cruziNADP+560.22260pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant K20S/R42FTrypanosoma cruziD-3-hydroxybutyratecosubstrate NADP+340.3990pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant K20S/R42FTrypanosoma cruziD-3-hydroxybutyratecosubstrate NAD+1.70.45pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant K20S/R42FTrypanosoma cruziNAD+370.4pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant K20S/R42FTrypanosoma cruziNADP+390.8247pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19TTrypanosoma cruziD-3-hydroxybutyratecosubstrate NADP+5.90.3517pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19TTrypanosoma cruziD-3-hydroxybutyratecosubstrate NAD+5.615.5pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19TTrypanosoma cruziNAD+6.71.74pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19TTrypanosoma cruziNADP+5.70.2522pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/K20STrypanosoma cruziD-3-hydroxybutyratecosubstrate NADP+360.23150pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/K20STrypanosoma cruziD-3-hydroxybutyratecosubstrate NAD+280.17160pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/K20STrypanosoma cruziNAD+200.3260pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/K20STrypanosoma cruziNADP+340.19180pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/K20S/C64YTrypanosoma cruziD-3-hydroxybutyratecosubstrate NADP+7.80.07999pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/K20S/C64YTrypanosoma cruziD-3-hydroxybutyratecosubstrate NAD+3.60.1426pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/K20S/C64YTrypanosoma cruziNAD+4.40.1333pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/K20S/C64YTrypanosoma cruziNADP+9.40.1187pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/K20S/R42FTrypanosoma cruziD-3-hydroxybutyratecosubstrate NADP+360.568pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/K20S/R42FTrypanosoma cruziD-3-hydroxybutyratecosubstrate NAD+411.139pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/K20S/R42FTrypanosoma cruziNAD+591.637pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/K20S/R42FTrypanosoma cruziNADP+50510pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/R42FTrypanosoma cruziD-3-hydroxybutyratecosubstrate NADP+33172pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/R42FTrypanosoma cruziD-3-hydroxybutyratecosubstrate NAD+1872.6pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/R42FTrypanosoma cruziNAD+2692.8pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R19T/R42FTrypanosoma cruziNADP+4258.3pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R42FTrypanosoma cruziD-3-hydroxybutyrate340.654pH 8.5Open
D-3-hydroxybutyrate dehydrogenase (TcHBDH)variant R42FTrypanosoma cruziNADP+280.3120pH 8.5Open