Values CatLog read from this paper
Extending Cofactor-Tethered Nanomachines to Complex Multienzyme Redox Cascades in Continuous Flow
PMID 42499152doi:10.1002/cssc.70914Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 37 records (57 values) from this paper: glucose dehydrogenase (BsGDH), alcohol dehydrogenase (TkADH), glyceraldehyde dehydrogenase (HsALDH) and 6 more from Bacillus subtilis, Herbaspirillum seropedicae, Thermococcus kodakarensis and 5 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 20 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
EC 1.1.1.1, EC 1.1.1.47, EC 1.1.1.48, EC 1.1.1.120, EC 1.1.1.359, EC 1.1.1.383
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alcohol dehydrogenase (BsADH / BstADH) | Geobacillus stearothermophilus | isobutyraldehyde | 46 | 2.7 | 17 calc. | pH 7.5, 37 °C | Open record: alcohol dehydrogenase (BsADH / BstADH), isobutyraldehyde |
| alcohol dehydrogenase (BsADH / BstADH) | Geobacillus stearothermophilus | NADH | — | 0.14 | — | pH 7.5, 37 °C | Open record: alcohol dehydrogenase (BsADH / BstADH), NADH |
| alcohol dehydrogenase (TkADH) | Thermococcus kodakarensis | D-Glyceraldehyde | — | 3.63 | — | pH 7.5 | Open record: alcohol dehydrogenase (TkADH), D-Glyceraldehyde |
| alcohol dehydrogenase (TkADH) | Thermococcus kodakarensis | glyceraldehyde | — | 3.6 | — | Open record: alcohol dehydrogenase (TkADH), glyceraldehyde | |
| alcohol dehydrogenase (TkADH) | Thermococcus kodakarensis | isobutyraldehyde | — | 12.5 | — | Open record: alcohol dehydrogenase (TkADH), isobutyraldehyde | |
| alcohol dehydrogenase (TkADH) | Thermococcus kodakarensis | isobutyraldehydefree enzyme | 5.9 | 14 | 0.42 calc. | pH 7.5, 37 °C | Open record: alcohol dehydrogenase (TkADH), isobutyraldehyde |
| alcohol dehydrogenase (TkADH) | Thermococcus kodakarensis | isobutyraldehydeADH domain of the fusion; TkADH-BsGDH fusion protein | 8.7 | 13 | 0.67 calc. | pH 7.5, 37 °C | Open record: alcohol dehydrogenase (TkADH), isobutyraldehyde |
| alcohol dehydrogenase (TkADH) | Thermococcus kodakarensis | NADHstep | — | 0.25 | — | Open record: alcohol dehydrogenase (TkADH), NADH | |
| alcohol dehydrogenase (TkADH) | Thermococcus kodakarensis | NADHdomain of the fusion; TkADH-BsGDH fusion protein | — | 0.25 | — | Open record: alcohol dehydrogenase (TkADH), NADH | |
| aldehyde dehydrogenase (TaALDH)variant M42 | Thermoplasma acidophilum | D-glyceraldehyde | 2.6 | 1.3 | 2.0 calc. | pH 7.5, 37 °C | Open record: aldehyde dehydrogenase (TaALDH) variant M42, D-glyceraldehyde |
| aldehyde dehydrogenase (TaALDH)variant M42 | Thermoplasma acidophilum | NAD+ | — | 1.5 | — | pH 7.5, 37 °C | Open record: aldehyde dehydrogenase (TaALDH) variant M42, NAD+ |
| glucose dehydrogenase (BsGDH)double mutant (mutations not printed) | Bacillus subtilis | D-glucosefree NAD+ added as cofactor | 87 | 3 | 29 calc. | Open record: glucose dehydrogenase (BsGDH) double mutant (mutations not printed), D-glucose | |
| glucose dehydrogenase (BsGDH)double mutant (mutations not printed) | Bacillus subtilis | D-glucosefree NAD+ added as cofactor; TkADH-BsGDH fusion protein | 142 | 5.7 | 25 calc. | Open record: glucose dehydrogenase (BsGDH) double mutant (mutations not printed), D-glucose | |
| glucose dehydrogenase (BsGDH)double mutant (mutations not printed) | Bacillus subtilis | D-glucoseno free cofactor added; N-terminal hexahistidine tag | 71 | 6 | 12 calc. | Open record: glucose dehydrogenase (BsGDH) double mutant (mutations not printed), D-glucose | |
| glucose dehydrogenase (BsGDH)double mutant (mutations not printed) | Bacillus subtilis | NAD+ | — | 0.9 | — | Open record: glucose dehydrogenase (BsGDH) double mutant (mutations not printed), NAD+ | |
| glucose dehydrogenase (BsGDH)double mutant (mutations not printed) | Bacillus subtilis | NAD+TkADH-BsGDH fusion protein; N-terminal hexahistidine tag | — | 0.9 | — | Open record: glucose dehydrogenase (BsGDH) double mutant (mutations not printed), NAD+ | |
| glucose dehydrogenase (BsGDH)thermostabilized double mutant (mutations not stated in the paper) | Bacillus subtilis | D-glucose50 mM HEPES pH 7.5; free enzyme with added free NAD+; NAD+ | 87 | 3 | 29 calc. | pH 7.5, 37 °C | Open record: glucose dehydrogenase (BsGDH) thermostabilized double mutant (mutations not stated in the paper), D-glucose |
| glucose dehydrogenase (BsGDH)thermostabilized double mutant (mutations not stated in the paper) | Bacillus subtilis | D-glucose50 mM HEPES pH 7.5; NAD+; TkADH-BsGDH fusion protein | 142 | 5.7 | 25 calc. | pH 7.5, 37 °C | Open record: glucose dehydrogenase (BsGDH) thermostabilized double mutant (mutations not stated in the paper), D-glucose |
| glucose dehydrogenase (BsGDH)thermostabilized double mutant (mutations not stated in the paper) | Bacillus subtilis | D-glucose50 mM buffer pH 7.5; no free NAD+ added; N-terminal His6-tag | 71 | 6 | 12 calc. | pH 7.5, 37 °C | Open record: glucose dehydrogenase (BsGDH) thermostabilized double mutant (mutations not stated in the paper), D-glucose |
| glucose dehydrogenase (BsGDH)thermostabilized double mutant (mutations not stated in the paper) | Bacillus subtilis | NAD+free enzyme | — | 0.9 | — | pH 7.5, 37 °C | Open record: glucose dehydrogenase (BsGDH) thermostabilized double mutant (mutations not stated in the paper), NAD+ |
| glucose dehydrogenase (BsGDH)thermostabilized double mutant (mutations not stated in the paper) | Bacillus subtilis | NAD+GDH domain of the fusion; TkADH-BsGDH fusion protein | — | 0.9 | — | pH 7.5, 37 °C | Open record: glucose dehydrogenase (BsGDH) thermostabilized double mutant (mutations not stated in the paper), NAD+ |
| glucose dehydrogenase (SsGDH) | Sulfolobus solfataricus | D-glucose | 7.1 | 1.9 | 3.7 calc. | pH 7.5, 37 °C | Open record: glucose dehydrogenase (SsGDH), D-glucose |
| glucose dehydrogenase (SsGDH) | Sulfolobus solfataricus | NAD+ | — | 0.2 | — | pH 7.5, 37 °C | Open record: glucose dehydrogenase (SsGDH), NAD+ |
| glyceraldehyde dehydrogenase (HsALDH)engineered variant optimized for glyceraldehyde (mutations not printed) | Herbaspirillum seropedicae | glyceraldehydefree NAD+ added as cofactor | 69 | 3.1 | 22 calc. | Open record: glyceraldehyde dehydrogenase (HsALDH) engineered variant optimized for glyceraldehyde (mutations not printed), glyceraldehyde | |
| glyceraldehyde dehydrogenase (HsALDH)engineered variant optimized for glyceraldehyde (mutations not printed) | Herbaspirillum seropedicae | glyceraldehydefree NAD+ added as cofactor; IaKARI-HsALDH fusion protein | 106 | 6.2 | 17 calc. | Open record: glyceraldehyde dehydrogenase (HsALDH) engineered variant optimized for glyceraldehyde (mutations not printed), glyceraldehyde | |
| glyceraldehyde dehydrogenase (HsALDH)engineered variant optimized for glyceraldehyde (mutations not printed) | Herbaspirillum seropedicae | glyceraldehydeno free cofactor added; N-terminal hexahistidine tag | 31 | 10.5 | 3.0 calc. | Open record: glyceraldehyde dehydrogenase (HsALDH) engineered variant optimized for glyceraldehyde (mutations not printed), glyceraldehyde | |
| glyceraldehyde dehydrogenase (HsALDH)engineered variant optimized for glyceraldehyde (mutations not printed) | Herbaspirillum seropedicae | NAD+ | — | 0.4 | — | Open record: glyceraldehyde dehydrogenase (HsALDH) engineered variant optimized for glyceraldehyde (mutations not printed), NAD+ | |
| glyceraldehyde dehydrogenase (HsALDH)engineered variant optimized for glyceraldehyde (mutations not printed) | Herbaspirillum seropedicae | NAD+IaKARI-HsALDH fusion protein; N-terminal hexahistidine tag | — | 0.8 | — | Open record: glyceraldehyde dehydrogenase (HsALDH) engineered variant optimized for glyceraldehyde (mutations not printed), NAD+ | |
| glyceraldehyde dehydrogenase / aldehyde dehydrogenase (HsALDH)engineered variant optimized for glyceraldehyde (mutations not stated in the paper) | Herbaspirillum seropedicae | D-glyceraldehyde50 mM HEPES pH 7.5; free enzyme with added free NAD+; NAD+ | 69 | 3.1 | 22 calc. | pH 7.5, 37 °C | Open record: glyceraldehyde dehydrogenase / aldehyde dehydrogenase (HsALDH) engineered variant optimized for glyceraldehyde (mutations not stated in the paper), D-glyceraldehyde |
| glyceraldehyde dehydrogenase / aldehyde dehydrogenase (HsALDH)engineered variant optimized for glyceraldehyde (mutations not stated in the paper) | Herbaspirillum seropedicae | D-glyceraldehyde50 mM HEPES pH 7.5; NAD+; IaKARI-HsALDH fusion protein | 106 | 6.2 | 17 calc. | pH 7.5, 37 °C | Open record: glyceraldehyde dehydrogenase / aldehyde dehydrogenase (HsALDH) engineered variant optimized for glyceraldehyde (mutations not stated in the paper), D-glyceraldehyde |
| glyceraldehyde dehydrogenase / aldehyde dehydrogenase (HsALDH)engineered variant optimized for glyceraldehyde (mutations not stated in the paper) | Herbaspirillum seropedicae | D-glyceraldehyde50 mM buffer pH 7.5; no free NAD+ added; N-terminal His6-tag | 31 | 10.5 | 3.0 calc. | pH 7.5, 37 °C | Open record: glyceraldehyde dehydrogenase / aldehyde dehydrogenase (HsALDH) engineered variant optimized for glyceraldehyde (mutations not stated in the paper), D-glyceraldehyde |
| glyceraldehyde dehydrogenase / aldehyde dehydrogenase (HsALDH)engineered variant optimized for glyceraldehyde (mutations not stated in the paper) | Herbaspirillum seropedicae | NAD+free enzyme | — | 0.4 | — | pH 7.5, 37 °C | Open record: glyceraldehyde dehydrogenase / aldehyde dehydrogenase (HsALDH) engineered variant optimized for glyceraldehyde (mutations not stated in the paper), NAD+ |
| glyceraldehyde dehydrogenase / aldehyde dehydrogenase (HsALDH)engineered variant optimized for glyceraldehyde (mutations not stated in the paper) | Herbaspirillum seropedicae | NAD+ALDH domain of the fusion; IaKARI-HsALDH fusion protein | — | 0.8 | — | pH 7.5, 37 °C | Open record: glyceraldehyde dehydrogenase / aldehyde dehydrogenase (HsALDH) engineered variant optimized for glyceraldehyde (mutations not stated in the paper), NAD+ |
| ketol-acid reductoisomerase (IaKARI)engineered monomeric variant (mutations not stated in the paper) | Ignisphaera aggregans | acetolactatefree enzyme | 1.23 | 3.3 | 0.37 calc. | pH 7.5, 37 °C | Open record: ketol-acid reductoisomerase (IaKARI) engineered monomeric variant (mutations not stated in the paper), acetolactate |
| ketol-acid reductoisomerase (IaKARI)engineered monomeric variant (mutations not stated in the paper) | Ignisphaera aggregans | acetolactateKARI domain of the fusion; IaKARI-HsALDH fusion protein | 1.8 | 0.37 | 4.9 calc. | pH 7.5, 37 °C | Open record: ketol-acid reductoisomerase (IaKARI) engineered monomeric variant (mutations not stated in the paper), acetolactate |
| ketol-acid reductoisomerase (MrKARI) | Meiothermus ruber | acetolactate | 1 | 1.4 | 0.71 calc. | pH 7.5, 37 °C | Open record: ketol-acid reductoisomerase (MrKARI), acetolactate |
| ketol-acid reductoisomerase (MrKARI) | Meiothermus ruber | NADH | — | 0.09 | — | pH 7.5, 37 °C | Open record: ketol-acid reductoisomerase (MrKARI), NADH |