Values CatLog read from this paper
Electrostatic Engineering of Phosphoketolase Enhances Activity on Small Nonphosphorylated Sugars and Improves Cell-Free ATP Regeneration from Inexpensive C2-Substrates
PMID 42560102doi:10.1021/acs.jpcb.6c02050Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (21 values) from this paper: phosphoketolase (Bad.F6Pkt), L-rhamnose isomerase (Ps.LrhI) from Bifidobacterium adolescentis, Pseudomonas stutzeri.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| L-rhamnose isomerase (Ps.LrhI) | Pseudomonas stutzeri | d-threose | — | 25.7 | — | pH 7, 37 °C | Open record: L-rhamnose isomerase (Ps.LrhI), d-threose |
| phosphoketolase (Bad.F6Pkt)variant H142N | Bifidobacterium adolescentis | d-erythrulose | — | 9 | 0.0116 | pH 6.5, 37 °C | Open record: phosphoketolase (Bad.F6Pkt) variant H142N, d-erythrulose |
| phosphoketolase (Bad.F6Pkt)variant H142N | Bifidobacterium adolescentis | glycolaldehyde | — | 42.3 | 0.0206 | pH 6.5, 37 °C | Open record: phosphoketolase (Bad.F6Pkt) variant H142N, glycolaldehyde |
| phosphoketolase (Bad.F6Pkt)variant H142N:E153D | Bifidobacterium adolescentis | d-erythrulose | — | 4.9 | 0.0094 | pH 6.5, 37 °C | Open record: phosphoketolase (Bad.F6Pkt) variant H142N:E153D, d-erythrulose |
| phosphoketolase (Bad.F6Pkt)variant H142N:E153D | Bifidobacterium adolescentis | glycolaldehyde | — | 4.4 | 0.0263 | pH 6.5, 37 °C | Open record: phosphoketolase (Bad.F6Pkt) variant H142N:E153D, glycolaldehyde |
| phosphoketolase (Bad.F6Pkt)variant H256Y:H260Y:H548Y | Bifidobacterium adolescentis | d-erythrulose | — | 16.9 | 0.0496 | pH 6.5, 37 °C | Open record: phosphoketolase (Bad.F6Pkt) variant H256Y:H260Y:H548Y, d-erythrulose |
| phosphoketolase (Bad.F6Pkt)variant H256Y:H260Y:H548Y | Bifidobacterium adolescentis | glycolaldehyde | — | 40.3 | 0.0191 | pH 6.5, 37 °C | Open record: phosphoketolase (Bad.F6Pkt) variant H256Y:H260Y:H548Y, glycolaldehyde |
| phosphoketolase (Bad.F6Pkt)variant H260Y:H548Y | Bifidobacterium adolescentis | d-erythrulose | — | 21.2 | 0.0549 | pH 6.5, 37 °C | Open record: phosphoketolase (Bad.F6Pkt) variant H260Y:H548Y, d-erythrulose |
| phosphoketolase (Bad.F6Pkt)variant H260Y:H548Y | Bifidobacterium adolescentis | glycolaldehyde | — | 40.9 | 0.019 | pH 6.5, 37 °C | Open record: phosphoketolase (Bad.F6Pkt) variant H260Y:H548Y, glycolaldehyde |
| phosphoketolase (Bad.F6Pkt)variant H548Y | Bifidobacterium adolescentis | d-erythrulose | — | 26.9 | 0.0354 | pH 6.5, 37 °C | Open record: phosphoketolase (Bad.F6Pkt) variant H548Y, d-erythrulose |
| phosphoketolase (Bad.F6Pkt)variant H548Y | Bifidobacterium adolescentis | glycolaldehyde | — | 43.4 | 0.0148 | pH 6.5, 37 °C | Open record: phosphoketolase (Bad.F6Pkt) variant H548Y, glycolaldehyde |