Values CatLog read from this paper
Creating a non-native bio-amidation activity in asparagine synthetase via biosensor-guided directed evolution for the synthesis of (S)-2-aminobutyramide
PMID 42683046doi:10.1039/d6sc04510cOpen in Browse
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CatLog read 2 records (6 values) from this paper: asparagine synthetase A (AsnA) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| asparagine synthetase A (AsnA) | Escherichia coli | L-aspartic acid | 1.071 | 1.64 | 0.6523 | 40 °C | Open record: asparagine synthetase A (AsnA), L-aspartic acid |
| asparagine synthetase A (AsnA)variant K77L/E120L/R255W (M2) | Escherichia coli | (S)-2-aminobutyric acid | 7.8 × 10⁻⁴ | 9.58 | 8.3 × 10⁻⁵ | 40 °C | Open record: asparagine synthetase A (AsnA) variant K77L/E120L/R255W (M2), (S)-2-aminobutyric acid |