Values CatLog read from this paper
Engineered polymerase-mediated efficient synthesis of site-specifically functionalized RNA and 2'-modified RNA oligonucleotides via genetic alphabet expansion
PMID 42722644doi:10.1038/s41467-026-76347-0Open in Browse
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CatLog read 18 records (54 values) from this paper: Stoffel fragment (SF) of Taq DNA polymerase, SFM4-6 from Thermus aquaticus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| SFM4-6SFM4-6 (laboratory-evolved Stoffel fragment mutant; mutations not printed in this paper) | Thermus aquaticus | dNaMTP | 0.0292 | 0.00169 | 17.3 | 50 °C | Open record: SFM4-6 SFM4-6 (laboratory-evolved Stoffel fragment mutant; mutations not printed in this paper), dNaMTP |
| SFM4-6SFM4-6 (laboratory-evolved Stoffel fragment mutant; mutations not printed in this paper) | Thermus aquaticus | dTPT3TP | 0.4885 | 2.2 × 10⁻⁴ | 2.22 × 10³ | 50 °C | Open record: SFM4-6 SFM4-6 (laboratory-evolved Stoffel fragment mutant; mutations not printed in this paper), dTPT3TP |
| SFM4-6SFM4-6 (laboratory-evolved Stoffel fragment mutant; mutations not printed in this paper) | Thermus aquaticus | dTTP | 0.542 | 1.7 × 10⁻⁴ | 3.18 × 10³ | 50 °C | Open record: SFM4-6 SFM4-6 (laboratory-evolved Stoffel fragment mutant; mutations not printed in this paper), dTTP |
| SFM4-6SFM4-6 (laboratory-evolved Stoffel fragment mutant; mutations not printed in this paper) | Thermus aquaticus | rNaMTP | 0.019 | 0.00232 | 8.2 | 50 °C | Open record: SFM4-6 SFM4-6 (laboratory-evolved Stoffel fragment mutant; mutations not printed in this paper), rNaMTP |
| SFM4-6SFM4-6 (laboratory-evolved Stoffel fragment mutant; mutations not printed in this paper) | Thermus aquaticus | rTPT3TP | 0.3405 | 8.3 × 10⁻⁴ | 410 | 50 °C | Open record: SFM4-6 SFM4-6 (laboratory-evolved Stoffel fragment mutant; mutations not printed in this paper), rTPT3TP |
| SFM4-6SFM4-6 (laboratory-evolved Stoffel fragment mutant; mutations not printed in this paper) | Thermus aquaticus | UTP | 0.597 | 2.6 × 10⁻⁴ | 2.3 × 10³ | 50 °C | Open record: SFM4-6 SFM4-6 (laboratory-evolved Stoffel fragment mutant; mutations not printed in this paper), UTP |
| Stoffel fragment (SF) of Taq DNA polymerasevariant SFM4-6 | — | (2’-deoxy-β-D-erythro-pentofuranosyl)-thieno[3,4]pyridine-2-thione 5’-triphosphate50 nM FAM-P-20/100 nM T55-A; 0-64 μM nucleoside triphosphate | 0.0562 | 0.00176 | 31.8 | 50 °C | Open record: Stoffel fragment (SF) of Taq DNA polymerase variant SFM4-6, (2’-deoxy-β-D-erythro-pentofuranosyl)-thieno[3,4]pyridine-2-thione 5’-triphosphate |
| Stoffel fragment (SF) of Taq DNA polymerasevariant SFM4-6 | — | (2’-deoxy-β-D-erythro-pentofuranosyl)-thieno[3,4]pyridine-2-thione 5’-triphosphate50 nM FAM-P-20/100 nM T55-T; 0-64 μM nucleoside triphosphate | 0.048 | 0.00162 | 29.7 | 50 °C | Open record: Stoffel fragment (SF) of Taq DNA polymerase variant SFM4-6, (2’-deoxy-β-D-erythro-pentofuranosyl)-thieno[3,4]pyridine-2-thione 5’-triphosphate |
| Stoffel fragment (SF) of Taq DNA polymerasevariant SFM4-6 | — | (2’-deoxy-β-D-erythro-pentofuranosyl)-thieno[3,4]pyridine-2-thione 5’-triphosphate50 nM FAM-P-20/100 nM T55-C; 0-64 μM nucleoside triphosphate | 0.037 | 0.00341 | 10.8 | 50 °C | Open record: Stoffel fragment (SF) of Taq DNA polymerase variant SFM4-6, (2’-deoxy-β-D-erythro-pentofuranosyl)-thieno[3,4]pyridine-2-thione 5’-triphosphate |
| Stoffel fragment (SF) of Taq DNA polymerasevariant SFM4-6 | — | (2’-deoxy-β-D-erythro-pentofuranosyl)-thieno[3,4]pyridine-2-thione 5’-triphosphate50 nM FAM-P-20/100 nM T55-G; 0-64 μM nucleoside triphosphate | 0.0372 | 0.00319 | 11.7 | 50 °C | Open record: Stoffel fragment (SF) of Taq DNA polymerase variant SFM4-6, (2’-deoxy-β-D-erythro-pentofuranosyl)-thieno[3,4]pyridine-2-thione 5’-triphosphate |
| Stoffel fragment (SF) of Taq DNA polymerasevariant SFM4-6 | — | (2’-deoxy-β-D-erythro-pentofuranosyl)-thieno[3,4]pyridine-2-thione 5’-triphosphate0-64 μM substrate | 0.0125 | 0.00336 | 3.7 | 50 °C | Open record: Stoffel fragment (SF) of Taq DNA polymerase variant SFM4-6, (2’-deoxy-β-D-erythro-pentofuranosyl)-thieno[3,4]pyridine-2-thione 5’-triphosphate |
| Stoffel fragment (SF) of Taq DNA polymerasevariant SFM4-6 | — | 2’-deoxy-2’-fluoroadenosine 5’-triphosphate | 0.4407 | 4.5 × 10⁻⁴ | 980 | 50 °C | Open record: Stoffel fragment (SF) of Taq DNA polymerase variant SFM4-6, 2’-deoxy-2’-fluoroadenosine 5’-triphosphate |
| Stoffel fragment (SF) of Taq DNA polymerasevariant SFM4-6 | — | 2’-O-methyladenosine 5’-triphosphate | 0.087 | 0.00102 | 85.3 | 50 °C | Open record: Stoffel fragment (SF) of Taq DNA polymerase variant SFM4-6, 2’-O-methyladenosine 5’-triphosphate |
| Stoffel fragment (SF) of Taq DNA polymerasevariant SFM4-6 | — | adenosine 5’-triphosphate | 0.3282 | 4.7 × 10⁻⁴ | 698 | 50 °C | Open record: Stoffel fragment (SF) of Taq DNA polymerase variant SFM4-6, adenosine 5’-triphosphate |
| Stoffel fragment (SF) of Taq DNA polymerasevariant SFM4-6 | — | rTPT3TP50 nM FAM-P-20/100 nM T55-A | 0.0188 | 0.00211 | 9 | 50 °C | Open record: Stoffel fragment (SF) of Taq DNA polymerase variant SFM4-6, rTPT3TP |
| Stoffel fragment (SF) of Taq DNA polymerasevariant SFM4-6 | — | rTPT3TP50 nM FAM-P-20/100 nM T55-T | 0.0188 | 0.00223 | 8.5 | 50 °C | Open record: Stoffel fragment (SF) of Taq DNA polymerase variant SFM4-6, rTPT3TP |
| Stoffel fragment (SF) of Taq DNA polymerasevariant SFM4-6 | — | rTPT3TP50 nM FAM-P-20/100 nM T55-C | 0.0162 | 0.0036 | 4.5 | 50 °C | Open record: Stoffel fragment (SF) of Taq DNA polymerase variant SFM4-6, rTPT3TP |
| Stoffel fragment (SF) of Taq DNA polymerasevariant SFM4-6 | — | rTPT3TP50 nM FAM-P-20/100 nM T55-G | 0.0178 | 0.0024 | 7.5 | 50 °C | Open record: Stoffel fragment (SF) of Taq DNA polymerase variant SFM4-6, rTPT3TP |