Values CatLog read from this paper
On the specificity and pH dependence of ficin-catalyzed hydrolyses. Some comparisons with bromelain specificity
PMID 4363754doi:10.1021/bi00707a005Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 38 records (108 values) from this paper: ficin II, ficin III, ficin.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ficin | — | ethyl N2-benzoyl-L-argininate | 4.1 | — | — | 25 °C | Open record: ficin, ethyl N2-benzoyl-L-argininate |
| ficin II | — | 2-nitrophenyl N-benzoylglycinate | 4.9 | 0.714 | 6.9 | pH 6, 25 °C | Open record: ficin II, 2-nitrophenyl N-benzoylglycinate |
| ficin II | — | 3-nitrophenyl N-benzoylglycinate | 5.15 | 0.0367 | 140 | pH 6, 25 °C | Open record: ficin II, 3-nitrophenyl N-benzoylglycinate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)-D-alaninate[E]0 = 5.19 x 10^-7 M | 0.45 | 0.2 | 2.25 | pH 6.36, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)-D-alaninate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)-D-alaninateconstants determined using Henri plots | 0.4 | 0.186 | 2.15 | pH 6.36, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)-D-alaninate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)-D-leucinate | 1.85 | 0.024 | 77.1 | pH 6.36, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)-D-leucinate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)-D-phenylalaninate | 1.2 | 0.128 | 9.4 | pH 6.36, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)-D-phenylalaninate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)-D-tyrosinate | 0.92 | 0.012 | 76.7 | pH 6.36, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)-D-tyrosinate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)-L-alaninate | 31 | 0.0059 | 5.25 × 10³ | pH 6, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)-L-alaninate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)-L-alaninate | 29.8 | 0.0258 | 1155 | pH 6.36, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)-L-alaninate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)-L-leucinate | 4.5 | 0.0122 | 369 | pH 6.36, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)-L-leucinate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)-L-phenylalaninate | 2.83 | 0.0377 | 75.1 | pH 6.36, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)-L-phenylalaninate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)-L-tyrosinate | 2.27 | 0.0034 | 668 | pH 6.36, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)-L-tyrosinate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)-L-valinate | 1.02 | 0.0327 | 31.2 | pH 6.36, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)-L-valinate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)glycinate3.17% (v/v) CH3CN; final cysteine 7.94 x 10^-5 M | 9.25 | 0.0134 | 690 | pH 6, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)glycinate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)glycinate | 12.4 | 0.0598 | 207 | pH 6.36, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)glycinate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)glycinate3.17% (v/v) acetonitrile; ficin II specific activity 51.0 | 9.57 | 0.0194 | 493 | pH 6, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)glycinate |
| ficin II | — | 4-nitrophenyl N-(benzyloxycarbonyl)glycylglycinate | 7.45 | 0.0172 | 433 | pH 6.36, 25 °C | Open record: ficin II, 4-nitrophenyl N-(benzyloxycarbonyl)glycylglycinate |
| ficin II | — | 4-nitrophenyl N-[(2E)-3-phenylprop-2-enoyl]glycinate | 8.64 | 0.0076 | 1.14 × 10³ | pH 6, 25 °C | Open record: ficin II, 4-nitrophenyl N-[(2E)-3-phenylprop-2-enoyl]glycinate |
| ficin II | — | 4-nitrophenyl N-acetylglycinate | 2.3 | 0.328 | 7 | pH 6, 25 °C | Open record: ficin II, 4-nitrophenyl N-acetylglycinate |
| ficin II | — | 4-nitrophenyl N-benzoylglycinate3.17% (v/v) CH3CN; final cysteine 7.94 x 10^-5 M | 5 | 0.0181 | 276 | pH 6, 25 °C | Open record: ficin II, 4-nitrophenyl N-benzoylglycinate |
| ficin II | — | 4-nitrophenyl N-benzoylglycinate3.17% (v/v) acetonitrile; ficin II specific activity 51.0 | 5.36 | 0.025 | 214 | pH 6, 25 °C | Open record: ficin II, 4-nitrophenyl N-benzoylglycinate |
| ficin II | — | 4-nitrophenyl N-formylglycinate | 2.5 | 1.1 | 2.3 | pH 6, 25 °C | Open record: ficin II, 4-nitrophenyl N-formylglycinate |
| ficin II | — | 4-nitrophenyl N2-(benzyloxycarbonyl)-L-lysinate | 35 | 0.0087 | 4.02 × 10³ | pH 6, 25 °C | Open record: ficin II, 4-nitrophenyl N2-(benzyloxycarbonyl)-L-lysinate |
| ficin II | — | 4-nitrophenyl N2-(benzyloxycarbonyl)-L-lysinate | 55 | 0.052 | 1058 | pH 6.36, 25 °C | Open record: ficin II, 4-nitrophenyl N2-(benzyloxycarbonyl)-L-lysinate |
| ficin II | — | phenyl N-benzoylglycinate | 5.1 | 0.261 | 19.5 | pH 6, 25 °C | Open record: ficin II, phenyl N-benzoylglycinate |
| ficin III | — | 2-nitrophenyl N-benzoylglycinate | 7.3 | 0.625 | 11.7 | pH 6, 25 °C | Open record: ficin III, 2-nitrophenyl N-benzoylglycinate |
| ficin III | — | 2-phenyl-4H-oxazol-5-one | 10.3 | 2.65 | 3.9 | pH 6.36, 25 °C | Open record: ficin III, 2-phenyl-4H-oxazol-5-one |
| ficin III | — | 2-phenyl-4H-oxazol-5-one | — | — | 3.11 | 25 °C | Open record: ficin III, 2-phenyl-4H-oxazol-5-one |
| ficin III | — | 3-nitrophenyl N-benzoylglycinate | 7.46 | 0.0171 | 436 | pH 6, 25 °C | Open record: ficin III, 3-nitrophenyl N-benzoylglycinate |
| ficin III | — | 4-nitrophenyl N-(benzyloxycarbonyl)glycinate | 13.34 | 0.0183 | 729 | pH 6, 25 °C | Open record: ficin III, 4-nitrophenyl N-(benzyloxycarbonyl)glycinate |
| ficin III | — | 4-nitrophenyl N-[(2E)-3-phenylprop-2-enoyl]glycinate | 12.28 | 0.0067 | 1.83 × 10³ | pH 6, 25 °C | Open record: ficin III, 4-nitrophenyl N-[(2E)-3-phenylprop-2-enoyl]glycinate |
| ficin III | — | 4-nitrophenyl N-acetylglycinate | 2.84 | 0.309 | 9.2 | pH 6, 25 °C | Open record: ficin III, 4-nitrophenyl N-acetylglycinate |
| ficin III | — | 4-nitrophenyl N-benzoylglycinate3.17% (v/v) CH3CN; final cysteine 7.94 x 10^-5 M | 7.47 | 0.0197 | 379 | pH 6, 25 °C | Open record: ficin III, 4-nitrophenyl N-benzoylglycinate |
| ficin III | — | 4-nitrophenyl N-benzoylglycinate | 10.2 | 0.1 | 102 | pH 6.36, 25 °C | Open record: ficin III, 4-nitrophenyl N-benzoylglycinate |
| ficin III | — | 4-nitrophenyl N-benzoylglycinate3.17% (v/v) acetonitrile; ficin III specific activity 68.2 | 7.27 | 0.0214 | 340 | pH 6, 25 °C | Open record: ficin III, 4-nitrophenyl N-benzoylglycinate |
| ficin III | — | methyl N2-benzoyl-L-argininate | 17.9 | — | — | 25 °C | Open record: ficin III, methyl N2-benzoyl-L-argininate |
| ficin III | — | p-nitrophenyl thiohippurate | 11 | 0.127 | 87 | pH 6.36, 25 °C | Open record: ficin III, p-nitrophenyl thiohippurate |