CatLogenzyme kinetics catalog

Values CatLog read from this paper

Separation and partial characterization of multiple forms of rat liver alcohol dehydrogenase

PMID 6354095doi:10.1016/0003-9861(83)90090-5Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 60 records (60 values) from this paper: alcohol dehydrogenase isoenzyme 1, alcohol dehydrogenase isoenzyme 2, alcohol dehydrogenase isoenzyme 3 and 1 more from Rattus norvegicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
alcohol dehydrogenase isoenzyme 1Rattus norvegicus3β,12α-dihydroxy-5β-cholanoic acid—0.315—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 1Rattus norvegicus3β,7α,12α-trihydroxy-5β-cholanoic acidoxidative direction; non-saturating substrate—0.149—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 1Rattus norvegicus3β,7α,12α-trihydroxy-5β-cholanoic acid0.5 M Tris-HCl buffer; steroid dispersed with Triton X-100—0.149—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 1Rattus norvegicus3β,7α-dihydroxy-5β-cholanoic acid—0.066—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 1Rattus norvegicus5α-androstan-17β-ol-3-one—0.04—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 1Rattus norvegicus5α-androstan-17β-ol-3-one (5α-dihydrotestosterone)—0.04—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 1Rattus norvegicus5β-androstan-3β-ol-17-oneoxidative direction; non-saturating substrate—0.164—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 1Rattus norvegicus5β-androstan-3β-ol-17-one0.5 M Tris-HCl buffer; steroid dispersed with Triton X-100—0.164—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 1Rattus norvegicusacetaldehyde—0.31—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 1Rattus norvegicusAcetaldehyde—0.31—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 1Rattus norvegicusethanol—1.37—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 1Rattus norvegicusNAD+—0.057—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 1Rattus norvegicusNADHreductive direction; 0.1 M sodium phosphate buffer pH 7.0—0.076—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 1Rattus norvegicusNADH0.1 M sodium phosphate buffer; CM-cellulose isoenzyme peak—0.076—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 1Rattus norvegicusnicotinamide adenine dinucleotide (oxidized)—0.057—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicus3β,12α-dihydroxy-5β-cholanoic acid—0.128—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicus3β,7α,12α-trihydroxy-5β-cholanoic acidoxidative direction; non-saturating substrate—0.16—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicus3β,7α,12α-trihydroxy-5β-cholanoic acid0.5 M Tris-HCl buffer; steroid dispersed with Triton X-100—0.16—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicus3β,7α-dihydroxy-5β-cholanoic acid—0.064—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicus5α-androstan-17β-ol-3-one—0.021—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicus5α-androstan-17β-ol-3-one (5α-dihydrotestosterone)—0.021—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicus5β-androstan-3β-ol-17-oneoxidative direction; non-saturating substrate—0.125—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicus5β-androstan-3β-ol-17-one0.5 M Tris-HCl buffer; steroid dispersed with Triton X-100—0.125—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicusacetaldehyde—0.23—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicusAcetaldehyde—0.23—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicusethanol—0.76—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicusNAD+—0.099—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicusNADHreductive direction; 0.1 M sodium phosphate buffer pH 7.0—0.071—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicusNADH0.1 M sodium phosphate buffer; CM-cellulose isoenzyme peak—0.071—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 2Rattus norvegicusnicotinamide adenine dinucleotide (oxidized)—0.099—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicus3β,12α-dihydroxy-5β-cholanoic acid—0.16—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicus3β,7α,12α-trihydroxy-5β-cholanoic acidoxidative direction; non-saturating substrate—0.182—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicus3β,7α,12α-trihydroxy-5β-cholanoic acid0.5 M Tris-HCl buffer; steroid dispersed with Triton X-100—0.182—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicus3β,7α-dihydroxy-5β-cholanoic acid—0.032—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicus5α-androstan-17β-ol-3-one—0.025—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicus5α-androstan-17β-ol-3-one (5α-dihydrotestosterone)—0.025—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicus5β-androstan-3β-ol-17-oneoxidative direction; non-saturating substrate—0.14—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicus5β-androstan-3β-ol-17-one0.5 M Tris-HCl buffer; steroid dispersed with Triton X-100—0.14—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicusacetaldehyde—0.217—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicusAcetaldehyde—0.217—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicusethanol—1.87—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicusNAD+—0.115—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicusNADHreductive direction; 0.1 M sodium phosphate buffer pH 7.0—0.091—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicusNADH0.1 M sodium phosphate buffer; CM-cellulose isoenzyme peak—0.091—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 3Rattus norvegicusnicotinamide adenine dinucleotide (oxidized)—0.115—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicus3β,12α-dihydroxy-5β-cholanoic acid—0.248—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicus3β,7α,12α-trihydroxy-5β-cholanoic acidoxidative direction; non-saturating substrate—0.35—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicus3β,7α,12α-trihydroxy-5β-cholanoic acid0.5 M Tris-HCl buffer; steroid dispersed with Triton X-100—0.35—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicus3β,7α-dihydroxy-5β-cholanoic acid—0.071—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicus5α-androstan-17β-ol-3-one—0.027—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicus5α-androstan-17β-ol-3-one (5α-dihydrotestosterone)—0.027—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicus5β-androstan-3β-ol-17-oneoxidative direction; non-saturating substrate—0.123—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicus5β-androstan-3β-ol-17-one0.5 M Tris-HCl buffer; steroid dispersed with Triton X-100—0.123—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicusacetaldehyde—0.276—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicusAcetaldehyde—0.276—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicusethanol—1.41—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicusNAD+—0.044—pH 7.2, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicusNADHreductive direction; 0.1 M sodium phosphate buffer pH 7.0—0.051—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicusNADH0.1 M sodium phosphate buffer; CM-cellulose isoenzyme peak—0.051—pH 7, 37 °COpen
alcohol dehydrogenase isoenzyme 4Rattus norvegicusnicotinamide adenine dinucleotide (oxidized)—0.044—pH 7.2, 37 °COpen