CatLogenzyme kinetics catalog

Values CatLog read from this paper

Purification and properties of thiol beta-lactamase. A mutant of pBR322 beta-lactamase in which the active site serine has been replaced with cysteine

PMID 6425288doi:10.1016/s0021-9258(17)42994-2Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 17 records (44 values) from this paper: RTEM-1 beta-lactamase (pBR322) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.5.2.6

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
RTEM-1 beta-lactamase (pBR322)Escherichia coliAmpicillin2 × 10³0.054.0 × 10⁴ calc.pH 7, 24 °COpen
RTEM-1 beta-lactamase (pBR322)Escherichia coliBenzylpenicillin50 mM sodium phosphate; spectrophotometric initial rates1.7 × 10³0.053.4 × 10⁴ calc.pH 7, 24 °COpen
RTEM-1 beta-lactamase (pBR322)Escherichia coliBenzylpenicillin1.4 × 10³0.082 × 10⁴pH 4.3, 24 °COpen
RTEM-1 beta-lactamase (pBR322)Escherichia coliBenzylpenicillin1.65 × 10³0.063 × 10⁴pH 5.4, 24 °COpen
RTEM-1 beta-lactamase (pBR322)Escherichia coliBenzylpenicillin50 mM phosphate buffer; 0.3 M NaCl; spectrophotometric assay1.7 × 10³0.044 × 10⁴pH 7, 24 °COpen
RTEM-1 beta-lactamase (pBR322)Escherichia coliBenzylpenicillin1.9 × 10³0.12 × 10⁴pH 7.8, 24 °COpen
RTEM-1 beta-lactamase (pBR322)Escherichia coliBenzylpenicillin2.4 × 10³2.51 × 10³pH 8.9, 24 °COpen
RTEM-1 beta-lactamase (pBR322)Escherichia coliCephaloridine1.7 × 10³11.7 × 10³ calc.pH 7, 24 °COpen
RTEM-1 beta-lactamase (pBR322)Escherichia coliNitrocefin9000.118.2 × 10³ calc.pH 7, 24 °COpen
RTEM-1 beta-lactamase (pBR322)variant S70CEscherichia coliAmpicillin500.12420 calc.pH 7, 24 °COpen
RTEM-1 beta-lactamase (pBR322)variant S70CEscherichia coliBenzylpenicillin50 mM sodium phosphate; spectrophotometric initial rates200.06330 calc.pH 7, 24 °COpen
RTEM-1 beta-lactamase (pBR322)variant S70CEscherichia coliBenzylpenicillin160.02700pH 4.3, 24 °COpen
RTEM-1 beta-lactamase (pBR322)variant S70CEscherichia coliBenzylpenicillin170.04400pH 5.4, 24 °COpen
RTEM-1 beta-lactamase (pBR322)variant S70CEscherichia coliBenzylpenicillin50 mM phosphate buffer; 0.3 M NaCl; spectrophotometric assay200.06300pH 7, 24 °COpen
RTEM-1 beta-lactamase (pBR322)variant S70CEscherichia coliBenzylpenicillin190.2290pH 7.8, 24 °COpen
RTEM-1 beta-lactamase (pBR322)variant S70CEscherichia coliBenzylpenicillin92.73pH 8.9, 24 °COpen
RTEM-1 beta-lactamase (pBR322)variant S70CEscherichia coliCephaloridine21.81.1 calc.pH 7, 24 °COpen