Values CatLog read from this paper
Multiple forms of the asclepains. Cysteinyl proteases from milkweed
PMID 6988008doi:10.1016/0005-2744(80)90284-3Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (20 values) from this paper: asclepain A1, asclepain A2, asclepain A3 and 7 more from Asclepias syriaca.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| asclepain A1 | Asclepias syriaca | N-benzyloxycarbonyllysine 4-nitrophenyl ester | 54.7 | 0.0114 | 4.80 × 10³ calc. | pH 6.3, 21 °C | Open record: asclepain A1, N-benzyloxycarbonyllysine 4-nitrophenyl ester |
| asclepain A2 | Asclepias syriaca | N-benzyloxycarbonyllysine 4-nitrophenyl ester | 46.2 | 0.0105 | 4.40 × 10³ calc. | pH 6.3, 21 °C | Open record: asclepain A2, N-benzyloxycarbonyllysine 4-nitrophenyl ester |
| asclepain A3 | Asclepias syriaca | N-benzyloxycarbonyllysine 4-nitrophenyl ester | 42.9 | 0.0142 | 3.02 × 10³ calc. | pH 6.3, 21 °C | Open record: asclepain A3, N-benzyloxycarbonyllysine 4-nitrophenyl ester |
| asclepain A4 | Asclepias syriaca | N-benzyloxycarbonyllysine 4-nitrophenyl ester | 59.6 | 0.0122 | 4.89 × 10³ calc. | pH 6.3, 21 °C | Open record: asclepain A4, N-benzyloxycarbonyllysine 4-nitrophenyl ester |
| asclepain A5 | Asclepias syriaca | N-benzyloxycarbonyllysine 4-nitrophenyl ester | 22.3 | 0.0097 | 2.3 × 10³ calc. | pH 6.3, 21 °C | Open record: asclepain A5, N-benzyloxycarbonyllysine 4-nitrophenyl ester |
| asclepain B1 | Asclepias syriaca | N-benzyloxycarbonyllysine 4-nitrophenyl ester | 34.6 | 0.0248 | 1.40 × 10³ calc. | pH 6.3, 21 °C | Open record: asclepain B1, N-benzyloxycarbonyllysine 4-nitrophenyl ester |
| asclepain B2 | Asclepias syriaca | N-benzyloxycarbonyllysine 4-nitrophenyl ester | 49.2 | 0.0389 | 1.26 × 10³ calc. | pH 6.3, 21 °C | Open record: asclepain B2, N-benzyloxycarbonyllysine 4-nitrophenyl ester |
| asclepain B3 | Asclepias syriaca | N-benzyloxycarbonyllysine 4-nitrophenyl ester | 25.8 | 0.0126 | 2.05 × 10³ calc. | pH 6.3, 21 °C | Open record: asclepain B3, N-benzyloxycarbonyllysine 4-nitrophenyl ester |
| asclepain B4 | Asclepias syriaca | N-benzyloxycarbonyllysine 4-nitrophenyl ester | 33.8 | 0.027 | 1.3 × 10³ calc. | pH 6.3, 21 °C | Open record: asclepain B4, N-benzyloxycarbonyllysine 4-nitrophenyl ester |
| asclepain B5 | Asclepias syriaca | N-benzyloxycarbonyllysine 4-nitrophenyl ester | 47.6 | 0.0272 | 1.75 × 10³ calc. | pH 6.3, 21 °C | Open record: asclepain B5, N-benzyloxycarbonyllysine 4-nitrophenyl ester |