Values CatLog read from this paper
Studies of catalysis by ribonuclease U2. Steady-state kinetics for transphosphorylation of oligonucleotide and synthetic substrates
PMID 7074019doi:10.1021/bi00531a025Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (53 values) from this paper: ribonuclease U2 (RNase U2) from Ustilago sphaerogena.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenosine 3'-(1-naphthyl phosphate) | 0.0852 | 0.114 | 0.747 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenosine 3'-(1-naphthyl phosphate) |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenosine 3'-(benzyl phosphate) | 0.0098 | 0.124 | 0.08 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenosine 3'-(benzyl phosphate) |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenosine 3'-(methyl phosphate) | 0.0032 | 0.126 | 0.025 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenosine 3'-(methyl phosphate) |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenylyl(3'→5')adenosine | 3.3 | 0.234 | 14.2 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenylyl(3'→5')adenosine |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenylyl(3'→5')adenosine 3'-phosphate | 24.3 | 0.0747 | 325 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenylyl(3'→5')adenosine 3'-phosphate |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenylyl(3'→5')cytidine | 23.8 | 0.424 | 56.2 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenylyl(3'→5')cytidine |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenylyl(3'→5')cytidine 3'-phosphate | 96.2 | 0.0774 | 1242 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenylyl(3'→5')cytidine 3'-phosphate |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenylyl(3'→5')cytidylyl(3'→5')guanosine | 141.2 | 0.1 | 1412 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenylyl(3'→5')cytidylyl(3'→5')guanosine |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenylyl(3'→5')cytidylyl(3'→5')guanosine 3'-phosphate | 228.7 | 0.129 | 1773 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenylyl(3'→5')cytidylyl(3'→5')guanosine 3'-phosphate |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenylyl(3'→5')guanosine | 6.3 | 0.3 | 21.2 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenylyl(3'→5')guanosine |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenylyl(3'→5')guanosine 3'-phosphate | 37.2 | 0.0411 | 905 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenylyl(3'→5')guanosine 3'-phosphate |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenylyl(3'→5')uridine | 7.3 | 0.319 | 23 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenylyl(3'→5')uridine |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenylyl(3'→5')uridine 3'-phosphate | 55 | 0.062 | 887 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenylyl(3'→5')uridine 3'-phosphate |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenylyl(3'→5')uridine 3'-phosphate | 98 | 0.0573 | 1.7 × 10³ calc. | 25 °C | Open record: ribonuclease U2 (RNase U2), adenylyl(3'→5')uridine 3'-phosphate |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenylyl(3'→5')uridylyl(3'→5')guanosine | 148.8 | 0.13 | 1145 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenylyl(3'→5')uridylyl(3'→5')guanosine |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | adenylyl(3'→5')uridylyl(3'→5')guanosine 3'-phosphate | 282.3 | 0.209 | 1352 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), adenylyl(3'→5')uridylyl(3'→5')guanosine 3'-phosphate |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | cytidylyl(3'→5')adenylyl(3'→5')guanosine 3'-phosphate | 59.5 | 0.141 | 422 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), cytidylyl(3'→5')adenylyl(3'→5')guanosine 3'-phosphate |
| ribonuclease U2 (RNase U2) | Ustilago sphaerogena | uridylyl(3'→5')adenylyl(3'→5')guanosine 3'-phosphate | 14.8 | 0.357 | 42 | pH 4.5, 25 °C | Open record: ribonuclease U2 (RNase U2), uridylyl(3'→5')adenylyl(3'→5')guanosine 3'-phosphate |