Values CatLog read from this paper
Analysis of the kinetic mechanism of enterococcal NADH peroxidase reveals catalytic roles for NADH complexes with both oxidized and two-electron-reduced enzyme forms
PMID 7578008doi:10.1021/bi00043a016Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (16 values) from this paper: NADH peroxidase from Enterococcus faecalis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| NADH peroxidase | Enterococcus faecalis | (4S)-[4-1H]NADH | 18.2 | — | — | pH 7, 5 °C | Open record: NADH peroxidase, (4S)-[4-1H]NADH |
| NADH peroxidase | Enterococcus faecalis | (4S)-[4-2H]NADH | 7.7 | — | — | pH 7, 5 °C | Open record: NADH peroxidase, (4S)-[4-2H]NADH |
| NADH peroxidase | Enterococcus faecalis | 3-acetylpyridine adenine dinucleotide (reduced) | 8.5 | 0.0027 | 3.1 × 10³ calc. | pH 7, 5 °C | Open record: NADH peroxidase, 3-acetylpyridine adenine dinucleotide (reduced) |
| NADH peroxidase | Enterococcus faecalis | 3-acetylpyridine adenine dinucleotide (reduced) | 1.5 | — | — | pH 8.5, 5 °C | Open record: NADH peroxidase, 3-acetylpyridine adenine dinucleotide (reduced) |
| NADH peroxidase | Enterococcus faecalis | H2O250 mM potassium phosphate; 0.1 M potassium acetate | — | 0.0141 | — | pH 7, 5 °C | Open record: NADH peroxidase, H2O2 |
| NADH peroxidase | Enterococcus faecalis | H2O2 | — | 0.0011 | — | pH 8.5, 5 °C | Open record: NADH peroxidase, H2O2 |
| NADH peroxidase | Enterococcus faecalis | H2O2steady-state; pH 7.0 phosphate/acetate buffer | — | — | 1.7 × 10³ | pH 7, 5 °C | Open record: NADH peroxidase, H2O2 |
| NADH peroxidase | Enterococcus faecalis | H2O2 | — | — | 4.2 × 10³ | pH 9, 25 °C | Open record: NADH peroxidase, H2O2 |
| NADH peroxidase | Enterococcus faecalis | NADH50 mM potassium phosphate; 0.5 mM EDTA; A340/A363 | 23.3 | 0.003 | 7.8 × 10³ calc. | pH 7, 5 °C | Open record: NADH peroxidase, NADH |
| NADH peroxidase | Enterococcus faecalis | NADH | 3.9 | 8 × 10⁻⁴ | 4.9 × 10³ calc. | pH 8.5, 5 °C | Open record: NADH peroxidase, NADH |
| NADH peroxidase | Enterococcus faecalis | NADHenzyme-monitored turnover; 21 µM enzyme; 0.18 mM NADH | 19.1 | — | — | pH 7, 5 °C | Open record: NADH peroxidase, NADH |
| NADH peroxidase | Enterococcus faecalis | NADH | 130.5 | — | — | pH 5, 25 °C | Open record: NADH peroxidase, NADH |
| NADH peroxidase | Enterococcus faecalis | NADH | 12.8 | — | — | 25 °C | Open record: NADH peroxidase, NADH |