Values CatLog read from this paper
Kinetic and mutagenic studies of the role of the active site residues Asp-50 and Glu-327 of Escherichia coli glutamine synthetase
PMID 7904829doi:10.1021/bi00169a008Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 48 records (56 values) from this paper: glutamine synthetase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glutamine synthetase | Escherichia coli | adenosine 5'-triphosphateTable 1 | — | 6 × 10⁻⁴ | — | pH 6.5, 25 °C | Open record: glutamine synthetase, adenosine 5'-triphosphate |
| glutamine synthetase | Escherichia coli | adenosine 5'-triphosphateTable 1 | — | 0.4 | — | pH 7.5, 25 °C | Open record: glutamine synthetase, adenosine 5'-triphosphate |
| glutamine synthetase | Escherichia coli | adenosine 5'-triphosphateTable 2 | — | — | 3.3 × 10³ | pH 6.5, 25 °C | Open record: glutamine synthetase, adenosine 5'-triphosphate |
| glutamine synthetase | Escherichia coli | adenosine 5'-triphosphateTable 2 | — | — | 100 | pH 7.5, 25 °C | Open record: glutamine synthetase, adenosine 5'-triphosphate |
| glutamine synthetase | Escherichia coli | ammoniumTable 1 | — | 0.1 | — | pH 6.5, 25 °C | Open record: glutamine synthetase, ammonium |
| glutamine synthetase | Escherichia coli | ammoniumTable 1 | — | 0.1 | — | pH 7.5, 25 °C | Open record: glutamine synthetase, ammonium |
| glutamine synthetase | Escherichia coli | ammoniumTable 2 | — | — | 20 | pH 6.5, 25 °C | Open record: glutamine synthetase, ammonium |
| glutamine synthetase | Escherichia coli | ammoniumTable 2 | — | — | 500 | pH 7.5, 25 °C | Open record: glutamine synthetase, ammonium |
| glutamine synthetase | Escherichia coli | L-glutamateTable 1 | 2 | 0.05 | 40 calc. | pH 6.5, 25 °C | Open record: glutamine synthetase, L-glutamate |
| glutamine synthetase | Escherichia coli | L-glutamateTable 1 | 50 | 5.5 | 9.1 calc. | pH 7.5, 25 °C | Open record: glutamine synthetase, L-glutamate |
| glutamine synthetase | Escherichia coli | L-glutamateTable 2 | — | — | 40 | pH 6.5, 25 °C | Open record: glutamine synthetase, L-glutamate |
| glutamine synthetase | Escherichia coli | L-glutamateTable 2 | — | — | 9.1 | pH 7.5, 25 °C | Open record: glutamine synthetase, L-glutamate |
| glutamine synthetasevariant D50A | Escherichia coli | adenosine 5'-triphosphateTable 1 | — | 0.0036 | — | pH 6.5, 25 °C | Open record: glutamine synthetase variant D50A, adenosine 5'-triphosphate |
| glutamine synthetasevariant D50A | Escherichia coli | adenosine 5'-triphosphateTable 1 | — | 0.6 | — | pH 7.5, 25 °C | Open record: glutamine synthetase variant D50A, adenosine 5'-triphosphate |
| glutamine synthetasevariant D50A | Escherichia coli | adenosine 5'-triphosphateTable 2 | — | — | 800 | pH 6.5, 25 °C | Open record: glutamine synthetase variant D50A, adenosine 5'-triphosphate |
| glutamine synthetasevariant D50A | Escherichia coli | adenosine 5'-triphosphateTable 2 | — | — | 50 | pH 7.5, 25 °C | Open record: glutamine synthetase variant D50A, adenosine 5'-triphosphate |
| glutamine synthetasevariant D50A | Escherichia coli | ammoniumTable 1 | — | 44 | — | pH 6.5, 25 °C | Open record: glutamine synthetase variant D50A, ammonium |
| glutamine synthetasevariant D50A | Escherichia coli | ammoniumTable 1 | — | 54 | — | pH 7.5, 25 °C | Open record: glutamine synthetase variant D50A, ammonium |
| glutamine synthetasevariant D50A | Escherichia coli | ammoniumTable 2 | — | — | 0.1 | pH 6.5, 25 °C | Open record: glutamine synthetase variant D50A, ammonium |
| glutamine synthetasevariant D50A | Escherichia coli | ammoniumTable 2 | — | — | 0.5 | pH 7.5, 25 °C | Open record: glutamine synthetase variant D50A, ammonium |
| glutamine synthetasevariant D50A | Escherichia coli | L-glutamateTable 1 | 3 | 0.2 | 15 calc. | pH 6.5, 25 °C | Open record: glutamine synthetase variant D50A, L-glutamate |
| glutamine synthetasevariant D50A | Escherichia coli | L-glutamateTable 1 | 29 | 8 | 3.6 calc. | pH 7.5, 25 °C | Open record: glutamine synthetase variant D50A, L-glutamate |
| glutamine synthetasevariant D50A | Escherichia coli | L-glutamateTable 2 | — | — | 15 | pH 6.5, 25 °C | Open record: glutamine synthetase variant D50A, L-glutamate |
| glutamine synthetasevariant D50A | Escherichia coli | L-glutamateTable 2 | — | — | 3.6 | pH 7.5, 25 °C | Open record: glutamine synthetase variant D50A, L-glutamate |
| glutamine synthetasevariant D50E | Escherichia coli | adenosine 5'-triphosphateTable 1 | — | 0.31 | — | pH 6.5, 25 °C | Open record: glutamine synthetase variant D50E, adenosine 5'-triphosphate |
| glutamine synthetasevariant D50E | Escherichia coli | adenosine 5'-triphosphateTable 1 | — | 0.6 | — | pH 7.5, 25 °C | Open record: glutamine synthetase variant D50E, adenosine 5'-triphosphate |
| glutamine synthetasevariant D50E | Escherichia coli | adenosine 5'-triphosphateTable 2 | — | — | 30 | pH 6.5, 25 °C | Open record: glutamine synthetase variant D50E, adenosine 5'-triphosphate |
| glutamine synthetasevariant D50E | Escherichia coli | adenosine 5'-triphosphateTable 2 | — | — | 0.2 | pH 7.5, 25 °C | Open record: glutamine synthetase variant D50E, adenosine 5'-triphosphate |
| glutamine synthetasevariant D50E | Escherichia coli | ammoniumTable 1 | — | 1.2 | — | pH 6.5, 25 °C | Open record: glutamine synthetase variant D50E, ammonium |
| glutamine synthetasevariant D50E | Escherichia coli | ammoniumTable 1 | — | 0.1 | — | pH 7.5, 25 °C | Open record: glutamine synthetase variant D50E, ammonium |
| glutamine synthetasevariant D50E | Escherichia coli | ammoniumTable 2 | — | — | 8.3 | pH 6.5, 25 °C | Open record: glutamine synthetase variant D50E, ammonium |
| glutamine synthetasevariant D50E | Escherichia coli | ammoniumTable 2 | — | — | 1 | pH 7.5, 25 °C | Open record: glutamine synthetase variant D50E, ammonium |
| glutamine synthetasevariant D50E | Escherichia coli | L-glutamateTable 1 | 10 | 5.4 | 1.9 calc. | pH 6.5, 25 °C | Open record: glutamine synthetase variant D50E, L-glutamate |
| glutamine synthetasevariant D50E | Escherichia coli | L-glutamateTable 1 | 0.1 | 54 | 0.0019 calc. | pH 7.5, 25 °C | Open record: glutamine synthetase variant D50E, L-glutamate |
| glutamine synthetasevariant D50E | Escherichia coli | L-glutamateTable 2 | — | — | 1.9 | pH 6.5, 25 °C | Open record: glutamine synthetase variant D50E, L-glutamate |
| glutamine synthetasevariant D50E | Escherichia coli | L-glutamateTable 2 | — | — | 0.002 | pH 7.5, 25 °C | Open record: glutamine synthetase variant D50E, L-glutamate |
| glutamine synthetasevariant E327A | Escherichia coli | adenosine 5'-triphosphateTable 1 | — | 0.001 | — | pH 6.5, 25 °C | Open record: glutamine synthetase variant E327A, adenosine 5'-triphosphate |
| glutamine synthetasevariant E327A | Escherichia coli | adenosine 5'-triphosphateTable 1 | — | 0.16 | — | pH 7.5, 25 °C | Open record: glutamine synthetase variant E327A, adenosine 5'-triphosphate |
| glutamine synthetasevariant E327A | Escherichia coli | adenosine 5'-triphosphateTable 2 | — | — | 1 × 10³ | pH 6.5, 25 °C | Open record: glutamine synthetase variant E327A, adenosine 5'-triphosphate |
| glutamine synthetasevariant E327A | Escherichia coli | adenosine 5'-triphosphateTable 2 | — | — | 200 | pH 7.5, 25 °C | Open record: glutamine synthetase variant E327A, adenosine 5'-triphosphate |
| glutamine synthetasevariant E327A | Escherichia coli | ammoniumTable 1 | — | 2.5 | — | pH 6.5, 25 °C | Open record: glutamine synthetase variant E327A, ammonium |
| glutamine synthetasevariant E327A | Escherichia coli | ammoniumTable 1 | — | 13 | — | pH 7.5, 25 °C | Open record: glutamine synthetase variant E327A, ammonium |
| glutamine synthetasevariant E327A | Escherichia coli | ammoniumTable 2 | — | — | 0.4 | pH 6.5, 25 °C | Open record: glutamine synthetase variant E327A, ammonium |
| glutamine synthetasevariant E327A | Escherichia coli | ammoniumTable 2 | — | — | 2 | pH 7.5, 25 °C | Open record: glutamine synthetase variant E327A, ammonium |
| glutamine synthetasevariant E327A | Escherichia coli | L-glutamateTable 1 | 1 | 0.04 | 25 calc. | pH 6.5, 25 °C | Open record: glutamine synthetase variant E327A, L-glutamate |
| glutamine synthetasevariant E327A | Escherichia coli | L-glutamateTable 1 | 25 | 2 | 12 calc. | pH 7.5, 25 °C | Open record: glutamine synthetase variant E327A, L-glutamate |
| glutamine synthetasevariant E327A | Escherichia coli | L-glutamateTable 2 | — | — | 25 | pH 6.5, 25 °C | Open record: glutamine synthetase variant E327A, L-glutamate |
| glutamine synthetasevariant E327A | Escherichia coli | L-glutamateTable 2 | — | — | 12.5 | pH 7.5, 25 °C | Open record: glutamine synthetase variant E327A, L-glutamate |